Ser571
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Home > Phosphorylation Site Page: > Ser571  -  PTP-PEST (human)

Site Information
KtVsLtPsPtTQVEt   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 1983755
Available spectra:  1 CST

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 12 , 13 , 14 , 15 , 16 , 17 ) , mass spectrometry (in vitro) ( 11 ) , mutation of modification site ( 11 )
Disease tissue studied:
breast cancer ( 6 , 7 , 10 ) , breast ductal carcinoma ( 6 ) , HER2 positive breast cancer ( 3 ) , luminal A breast cancer ( 3 ) , luminal B breast cancer ( 3 ) , breast cancer, triple negative ( 3 ) , leukemia ( 16 ) , chronic myelogenous leukemia ( 16 ) , lung cancer ( 8 , 10 ) , non-small cell lung cancer ( 8 , 10 ) , non-small cell lung adenocarcinoma ( 8 ) , neuroblastoma ( 1 ) , ovarian cancer ( 6 ) , melanoma skin cancer ( 14 )
Relevant cell line - cell type - tissue:
293E (epithelial) ( 12 ) , A498 (renal) ( 13 ) , breast ( 3 , 6 ) , BT-20 (breast cell) ( 10 ) , BT-549 (breast cell) ( 10 ) , Cal-12T (pulmonary) ( 8 ) , Calu 6 (pulmonary) ( 10 ) , CLB-Bar ( 1 ) , H2009 (pulmonary) ( 10 ) , H2077 (pulmonary) ( 10 ) , H2887 (pulmonary) ( 10 ) , H322M (pulmonary) ( 10 ) , HCC1359 (pulmonary) ( 10 ) , HCC1937 (breast cell) ( 10 ) , HCC2279 (pulmonary) ( 10 ) , HCC366 (pulmonary) ( 10 ) , HCC4006 (pulmonary) ( 10 ) , HCC44 (pulmonary) ( 8 ) , HCC78 (pulmonary) ( 10 ) , HCC827 (pulmonary) ( 10 ) , HEK293T (epithelial) ( 11 ) , HeLa (cervical) ( 2 , 5 , 15 ) , HMLER ('stem, breast cancer') [CXCR4 (human), knockdown] ( 7 ) , HMLER ('stem, breast cancer') ( 7 ) , HOP62 (pulmonary) ( 10 ) , K562 (erythroid) ( 9 , 16 ) , LCLC-103H (pulmonary) ( 10 ) , LOU-NH91 (squamous) ( 10 ) , lung ( 8 ) , MCF-7 (breast cell) ( 4 , 10 ) , MDA-MB-231 (breast cell) ( 10 ) , MDA-MB-468 (breast cell) ( 10 ) , NCI-H1395 (pulmonary) ( 10 ) , NCI-H1437 (pulmonary) ( 8 ) , NCI-H1568 (pulmonary) ( 10 ) , NCI-H1648 (pulmonary) ( 10 ) , NCI-H1666 (pulmonary) ( 8 ) , NCI-H2030 (pulmonary) ( 10 ) , NCI-H2172 (pulmonary) ( 10 ) , NCI-H2405 (pulmonary) ( 8 ) , NCI-H322 (pulmonary) ( 10 ) , NCI-H460 (pulmonary) ( 10 ) , NCI-H647 (pulmonary) ( 10 ) , ovary ( 6 ) , platelet-blood ( 17 ) , skin ( 14 )

Upstream Regulation
Regulatory protein:
MEK1 (human) ( 11 )
Putative in vivo kinases:
ERK1 (human) ( 11 ) , ERK2 (human) ( 11 )
Kinases, in vitro:
ERK1 (human) ( 11 ) , ERK2 (human) ( 11 )
Treatments:
U0126 ( 11 )

Downstream Regulation
Effects of modification on PTP-PEST:
molecular association, regulation ( 11 ) , phosphorylation ( 11 )
Effects of modification on biological processes:
carcinogenesis, induced ( 11 ) , cell adhesion, inhibited ( 11 ) , cell motility, induced ( 11 )
Induce interaction with:
FAK (human) ( 11 ) , PIN1 (human) ( 11 )

Disease / Diagnostics Relevance
Relevant diseases:
lung cancer ( 11 )

References 

1

Borenäs M, et al. (2024) ALK signaling primes the DNA damage response sensitizing ALK-driven neuroblastoma to therapeutic ATR inhibition. Proc Natl Acad Sci U S A 121, e2315242121
38154064   Curated Info

2

Huang H, et al. (2016) Simultaneous Enrichment of Cysteine-containing Peptides and Phosphopeptides Using a Cysteine-specific Phosphonate Adaptable Tag (CysPAT) in Combination with titanium dioxide (TiO2) Chromatography. Mol Cell Proteomics 15, 3282-3296
27281782   Curated Info

3

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

4

Sacco F, et al. (2016) Deep Proteomics of Breast Cancer Cells Reveals that Metformin Rewires Signaling Networks Away from a Pro-growth State. Cell Syst 2, 159-71
27135362   Curated Info

5

Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94
25159151   Curated Info

6

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

7

Yi T, et al. (2014) Quantitative phosphoproteomic analysis reveals system-wide signaling pathways downstream of SDF-1/CXCR4 in breast cancer stem cells. Proc Natl Acad Sci U S A 111, E2182-90
24782546   Curated Info

8

Rikova K, Hall B (2013) CST Curation Set: 20741, 21168, 30173, 30174, 30175; Year: 2013; Biosample/Treatment: cell line, H1666, CAL-12T, H2405, HCC44, H1437; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY, p[ST], RXXp[ST], pSQ, p[ST]QG, LXRXXp[ST], p[ST]P
Curated Info

9

Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71
23186163   Curated Info

10

Klammer M, et al. (2012) Phosphosignature predicts dasatinib response in non-small cell lung cancer. Mol Cell Proteomics 11, 651-68
22617229   Curated Info

11

Zheng Y, et al. (2011) Ras-Induced and Extracellular Signal-Regulated Kinase 1 and 2 Phosphorylation-Dependent Isomerization of Protein Tyrosine Phosphatase (PTP)-PEST by PIN1 Promotes FAK Dephosphorylation by PTP-PEST. Mol Cell Biol 31, 4258-69
21876001   Curated Info

12

Hsu PP, et al. (2011) The mTOR-regulated phosphoproteome reveals a mechanism of mTORC1-mediated inhibition of growth factor signaling. Science 332, 1317-22
21659604   Curated Info

13

Schreiber TB, et al. (2010) An integrated phosphoproteomics work flow reveals extensive network regulation in early lysophosphatidic acid signaling. Mol Cell Proteomics 9, 1047-62
20071362   Curated Info

14

Tucker M (2010) CST Curation Set: 9783; Year: 2010; Biosample/Treatment: tissue, skin/untreated; Disease: melanoma skin cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
Curated Info

15

Dephoure N, et al. (2008) A quantitative atlas of mitotic phosphorylation. Proc Natl Acad Sci U S A 105, 10762-7
18669648   Curated Info

16

Stokes M (2008) CST Curation Set: 4390; Year: 2008; Biosample/Treatment: cell line, K562/untreated; Disease: chronic myelogenous leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY])
Curated Info

17

Zahedi RP, et al. (2008) Phosphoproteome of resting human platelets. J Proteome Res 7, 526-34
18088087   Curated Info

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