Thr509
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Home > Phosphorylation Site Page: > Thr509  -  CRMP-1 (human)

Site Information
PVyEVPAtPKyAtPA   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 454314

In vivo Characterization
Methods used to characterize site in vivo:
labeling using "Shokat-modified" upstream kinase ( 5 ) , mass spectrometry ( 1 , 2 , 3 , 4 , 7 ) , mass spectrometry (in vitro) ( 5 ) , microscopy-colocalization with upstream kinase ( 6 ) , mutation of modification site ( 6 , 8 ) , phospho-antibody ( 8 ) , western blotting ( 8 )
Disease tissue studied:
HER2 positive breast cancer ( 1 ) , luminal A breast cancer ( 1 ) , luminal B breast cancer ( 1 ) , breast cancer, triple negative ( 1 ) , neuroblastoma ( 8 ) , FTLD ( 4 )
Relevant cell line - cell type - tissue:
'brain, cerebral cortex' ( 4 ) , 'brain, hippocampus' ( 8 ) , breast ( 1 ) , HeLa (cervical) ( 3 ) , N1E-115 (neuron) ( 8 ) , neuron-'brain, cerebral cortex' ( 8 ) , neuron-'brain, cerebral cortex' [CRMP-1 (mouse)] ( 6 ) , SEM (B lymphocyte) ( 7 ) , SH-SY5Y (neural crest) [LRRK2 (human), transfection, over-expression of LRRK2(G2019S)] ( 2 ) , SH-SY5Y (neural crest) ( 8 )

Upstream Regulation
Putative in vivo kinases:
CDK5 (mouse) ( 6 , 8 )
Kinases, in vitro:
CDK2 (human) ( 5 ) , CDK5 (human) ( 8 )
Treatments:
CT-99021 ( 8 )

Downstream Regulation
Effects of modification on biological processes:
cytoskeletal reorganization ( 6 )

References 

1

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

2

Luerman GC, et al. (2014) Phosphoproteomic evaluation of pharmacological inhibition of leucine-rich repeat kinase 2 reveals significant off-target effects of LRRK-2-IN-1. J Neurochem 128, 561-76
24117733   Curated Info

3

Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5
21712546   Curated Info

4

Herskowitz JH, et al. (2010) Phosphoproteomic Analysis Reveals Site-Specific Changes in GFAP and NDRG2 Phosphorylation in Frontotemporal Lobar Degeneration. J Proteome Res 9, 6368-79
20886841   Curated Info

5

Chi Y, et al. (2008) Identification of CDK2 substrates in human cell lysates. Genome Biol 9, R149
18847512   Curated Info

6

Yamashita N, et al. (2007) Regulation of spine development by semaphorin3A through cyclin-dependent kinase 5 phosphorylation of collapsin response mediator protein 1. J Neurosci 27, 12546-54
18003833   Curated Info

7

Moritz A (2006) CST Curation Set: 1819; Year: 2006; Biosample/Treatment: cell line, SEM/Flt3 inhibitor; Disease: acute lymphocytic leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
Curated Info

8

Cole AR, et al. (2006) Distinct priming kinases contribute to differential regulation of collapsin response mediator proteins by glycogen synthase kinase-3 in vivo. J Biol Chem 281, 16591-8
16611631   Curated Info