Thr77
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Home > Phosphorylation Site Page: > Thr77  -  CCNE1 (human)

Site Information
DPCsLIPtPDKEDDD   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 454411

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 4 ) , mutation of modification site ( 2 , 4 , 5 ) , peptide sequencing ( 4 ) , western blotting ( 2 , 5 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 5 ) , 293T (epithelial) ( 2 , 4 ) , 76NE6 (epithelial) ( 2 )

Upstream Regulation
Kinases, in vitro:
GSK3B (human) ( 5 )

Downstream Regulation
Effects of modification on CCNE1:
molecular association, regulation ( 3 , 4 ) , protein degradation ( 4 , 5 )
Induce interaction with:
FBXW7 (human) ( 3 , 4 )

References 

1

Davis RJ, Swanger J, Hughes BT, Clurman BE (2017) The PP2A-B56 Phosphatase Opposes Cyclin E Autocatalytic Degradation via Site-Specific Dephosphorylation. Mol Cell Biol 37
28137908   Curated Info

2

Mull BB, Cox J, Bui T, Keyomarsi K (2009) Post-translational modification and stability of low molecular weight cyclin E. Oncogene 28, 3167-76
19561641   Curated Info

3

Hao B, et al. (2007) Structure of a Fbw7-Skp1-cyclin E complex: multisite-phosphorylated substrate recognition by SCF ubiquitin ligases. Mol Cell 26, 131-43
17434132   Curated Info

4

Ye X, et al. (2004) Recognition of phosphodegron motifs in human cyclin E by the SCF(Fbw7) ubiquitin ligase. J Biol Chem 279, 50110-9
15364936   Curated Info

5

Welcker M, et al. (2003) Multisite phosphorylation by Cdk2 and GSK3 controls cyclin E degradation. Mol Cell 12, 381-92
14536078   Curated Info