Ser1257

Site Information
sLsPsPPsPIEKtPL SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 454136

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 1 , 2 , 3 , 4 ) , phospho-antibody ( 5 ) , western blotting ( 5 )
Relevant cell line - cell type - tissue:	'brain, brainstem' ( 3 ) , 'brain, cerebellum' ( 3 ) , 'brain, cerebral cortex' ( 3 ) , 'kidney, inner medulla' ( 4 ) , adipose tissue ( 3 ) , blood ( 3 ) , CHO (fibroblast) ( 5 ) , COS (fibroblast) ( 5 ) , embryo ( 5 ) , heart ( 3 ) , IEC-6 (epithelial) ( 1 ) , intestine ( 3 ) , kidney ( 3 ) , lung ( 3 ) , neuron-spinal cord ( 5 ) , PC-12 (chromaffin) [TrkA (rat), transfection] ( 2 ) , PC-12 (chromaffin) ( 2 ) , spleen ( 3 ) , stomach ( 3 ) , testis ( 3 )

In vivo Characterization

Methods used to characterize site in vivo:

mass spectrometry ( 1 , 2 , 3 , 4 ) , phospho-antibody ( 5 ) , western blotting ( 5 )

Relevant cell line - cell type - tissue:

'brain, brainstem' ( 3 ) , 'brain, cerebellum' ( 3 ) , 'brain, cerebral cortex' ( 3 ) , 'kidney, inner medulla' ( 4 ) , adipose tissue ( 3 ) , blood ( 3 ) , CHO (fibroblast) ( 5 ) , COS (fibroblast) ( 5 ) , embryo ( 5 ) , heart ( 3 ) , IEC-6 (epithelial) ( 1 ) , intestine ( 3 ) , kidney ( 3 ) , lung ( 3 ) , neuron-spinal cord ( 5 ) , PC-12 (chromaffin) [TrkA (rat), transfection] ( 2 ) , PC-12 (chromaffin) ( 2 ) , spleen ( 3 ) , stomach ( 3 ) , testis ( 3 )

Upstream Regulation
Treatments:	PDGF ( 2 )

References
1	Courcelles M, et al. (2013) Phosphoproteome dynamics reveal novel ERK1/2 MAP kinase substrates with broad spectrum of functions. Mol Syst Biol 9, 669 23712012 Curated Info
2	Biarc J, Chalkley RJ, Burlingame AL, Bradshaw RA (2012) The induction of serine/threonine protein phosphorylations by a PDGFR/TrkA chimera in stably transfected PC12 cells. Mol Cell Proteomics 11, 15-30 22027198 Curated Info
3	Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876 22673903 Curated Info
4	Hoffert JD, et al. (2006) Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites. Proc Natl Acad Sci U S A 103, 7159-64 16641100 Curated Info
5	Trivedi N, et al. (2005) Glycogen synthase kinase-3beta phosphorylation of MAP1B at Ser1260 and Thr1265 is spatially restricted to growing axons. J Cell Sci 118, 993-1005 15731007 Curated Info