Ser850

Site Information
NrAVsLDsPVSVGSG SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 454106

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 1 , 2 , 3 , 4 , 6 , 7 , 8 ) , mutation of modification site ( 5 )
Relevant cell line - cell type - tissue:	'fat, brown' ( 4 ) , ES (stem) ( 5 ) , heart ( 4 ) , Hepa 1-6 (epithelial) ( 8 ) , kidney ( 4 , 5 ) , liver ( 1 , 4 , 5 ) , lung ( 4 ) , macrophage-bone marrow ( 6 ) , macrophage-bone marrow [DUSP1 (mouse), homozygous knockout] ( 6 ) , macrophage-peritoneum [MPRIP (mouse), homozygous knockout] ( 2 ) , MEF (fibroblast) [TSC2 (mouse), homozygous knockout] ( 3 ) , MEF (fibroblast) ( 5 ) , pancreas ( 4 ) , RAW 267.4 (macrophage) ( 7 ) , spleen ( 4 )

In vivo Characterization

Methods used to characterize site in vivo:

mass spectrometry ( 1 , 2 , 3 , 4 , 6 , 7 , 8 ) , mutation of modification site ( 5 )

Relevant cell line - cell type - tissue:

'fat, brown' ( 4 ) , ES (stem) ( 5 ) , heart ( 4 ) , Hepa 1-6 (epithelial) ( 8 ) , kidney ( 4 , 5 ) , liver ( 1 , 4 , 5 ) , lung ( 4 ) , macrophage-bone marrow ( 6 ) , macrophage-bone marrow [DUSP1 (mouse), homozygous knockout] ( 6 ) , macrophage-peritoneum [MPRIP (mouse), homozygous knockout] ( 2 ) , MEF (fibroblast) [TSC2 (mouse), homozygous knockout] ( 3 ) , MEF (fibroblast) ( 5 ) , pancreas ( 4 ) , RAW 267.4 (macrophage) ( 7 ) , spleen ( 4 )

Upstream Regulation
Treatments:	LPS ( 6 )

Downstream Regulation
Effects of modification on biological processes:	carcinogenesis, inhibited ( 5 ) , cell differentiation, induced ( 5 ) , cell growth, inhibited ( 5 ) , signaling pathway regulation ( 5 ) , transcription, inhibited ( 5 )

Disease / Diagnostics Relevance
Relevant diseases:	liver cancer ( 5 )

References
1	Wilson-Grady JT, Haas W, Gygi SP (2013) Quantitative comparison of the fasted and re-fed mouse liver phosphoproteomes using lower pH reductive dimethylation. Methods 61, 277-86 23567750 Curated Info
2	Wu X, et al. (2012) Investigation of receptor interacting protein (RIP3)-dependent protein phosphorylation by quantitative phosphoproteomics. Mol Cell Proteomics 11, 1640-51 22942356 Curated Info
3	Yu Y, et al. (2011) Phosphoproteomic analysis identifies Grb10 as an mTORC1 substrate that negatively regulates insulin signaling. Science 332, 1322-6 21659605 Curated Info
4	Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89 21183079 Curated Info
5	York B, et al. (2010) Reprogramming the posttranslational code of SRC-3 confers a switch in mammalian systems biology. Proc Natl Acad Sci U S A 107, 11122-7 20534466 Curated Info
6	Weintz G, et al. (2010) The phosphoproteome of toll-like receptor-activated macrophages. Mol Syst Biol 6, 371 20531401 Curated Info
7	Trost M, et al. (2009) The phagosomal proteome in interferon-gamma-activated macrophages. Immunity 30, 143-54 19144319 Curated Info
8	Pan C, Gnad F, Olsen JV, Mann M (2008) Quantitative phosphoproteome analysis of a mouse liver cell line reveals specificity of phosphatase inhibitors. Proteomics 8, 4534-46 18846507 Curated Info