Ser12
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Home > Phosphorylation Site Page: > Ser12  -  RFA2 (human)

Site Information
GFEsyGsssyGGAGG   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 1854602

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 3 ) , electrophoretic mobility shift ( 2 , 3 ) , immunoprecipitation ( 1 ) , mutation of modification site ( 1 , 2 ) , peptide sequencing ( 3 ) , phosphopeptide mapping ( 3 ) , western blotting ( 1 )
Disease tissue studied:
bone cancer ( 2 )
Relevant cell line - cell type - tissue:
fibroblast ( 1 ) , HeLa (cervical) ( 3 ) , U2OS (bone cell) ( 2 )

Upstream Regulation
Treatments:
UV ( 3 )

Downstream Regulation
Effects of modification on RFA2:
intracellular localization ( 2 ) , molecular association, regulation ( 1 )
Effects of modification on biological processes:
cell growth, altered ( 2 ) , DNA repair, induced ( 1 )
Induce interaction with:
WRN (human) ( 1 )

References 

1

Machwe A, et al. (2011) Molecular cooperation between the Werner syndrome protein and replication protein A in relation to replication fork blockage. J Biol Chem 286, 3497-508
21107010   Curated Info

2

Olson E, et al. (2006) RPA2 is a direct downstream target for ATR to regulate the S-phase checkpoint. J Biol Chem 281, 39517-33
17035231   Curated Info

3

Zernik-Kobak M, et al. (1997) Sites of UV-induced phosphorylation of the p34 subunit of replication protein A from HeLa cells. J Biol Chem 272, 23896-904
9295339   Curated Info