Ser55
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Home > Phosphorylation Site Page: > Ser55  -  BIM (mouse)

Site Information
GDRCPHGsPQGPLAP   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 454235

In vivo Characterization
Methods used to characterize site in vivo:
2D analysis ( 3 , 4 ) , flow cytometry ( 1 ) , immunoprecipitation ( 1 ) , mass spectrometry ( 2 ) , mutation of modification site ( 1 , 3 , 4 , 5 ) , phospho-antibody ( 3 , 5 ) , western blotting ( 1 , 3 , 5 )
Relevant cell line - cell type - tissue:
'3T3-L1, differentiated' (adipocyte) ( 2 ) , 293 (epithelial) ( 3 ) , epithelial-mammary gland ( 1 ) , FL5.12 (lymphoid) [BIM (mouse)] ( 5 ) , HEK293T (epithelial) ( 1 ) , HeLa (cervical) ( 4 ) , kidney ( 1 ) , MEF (fibroblast) ( 3 ) , RAW 264 (macrophage) ( 4 )

Upstream Regulation
Putative in vivo kinases:
ERK1 (mouse) ( 5 ) , ERK2 (mouse) ( 5 )
Treatments:
calyculin_A ( 4 ) , IL-3 ( 5 ) , insulin ( 2 ) , LPS ( 4 ) , LY294002 ( 5 ) , PD98059 ( 5 ) , SB203580 ( 5 ) , serum ( 3 ) , SP600125 ( 5 ) , U0126 ( 5 )

Downstream Regulation
Effects of modification on BIM:
molecular association, regulation ( 5 ) , protein degradation ( 3 , 4 )
Effects of modification on biological processes:
apoptosis, altered ( 5 ) , apoptosis, inhibited ( 3 , 4 )
Inhibit interaction with:
BAX (mouse) ( 5 )

References 

1

Girnius N, Davis RJ (2017) JNK Promotes Epithelial Cell Anoikis by Transcriptional and Post-translational Regulation of BH3-Only Proteins. Cell Rep 21, 1910-1921
29141222   Curated Info

2

Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2. Cell Metab 17, 1009-20
23684622   Curated Info

3

Hübner A, Barrett T, Flavell RA, Davis RJ (2008) Multisite phosphorylation regulates Bim stability and apoptotic activity. Mol Cell 30, 415-25
18498746   Curated Info

4

Häcker G, Suttner K, Harada H, Kirschnek S (2006) TLR-dependent Bim phosphorylation in macrophages is mediated by ERK and is connected to proteasomal degradation of the protein. Int Immunol 18, 1749-57
17105963   Curated Info

5

Harada H, Quearry B, Ruiz-Vela A, Korsmeyer SJ (2004) Survival factor-induced extracellular signal-regulated kinase phosphorylates BIM, inhibiting its association with BAX and proapoptotic activity. Proc Natl Acad Sci U S A 101, 15313-7
15486085   Curated Info