Thr89

Site Information
RtLtLVDtGIGMtkA SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 18495101

In vivo Characterization
Methods used to characterize site in vivo:	electrophoretic mobility shift ( 3 ) , immunoassay ( 1 ) , immunoprecipitation ( 1 ) , mass spectrometry ( 2 , 3 , 4 ) , mutation of modification site ( 1 ) , western blotting ( 1 , 3 )
Disease tissue studied:	gastric cancer ( 4 ) , gastric carcinoma ( 4 ) , lung cancer ( 2 ) , non-small cell lung adenocarcinoma ( 2 ) , prostate cancer ( 1 )
Relevant cell line - cell type - tissue:	293 (epithelial) [CAMK1B (human), transfection] ( 3 ) , 293 (epithelial) ( 1 ) , LNCaP (prostate cell) ( 1 ) , lung ( 2 ) , MKN-45 (gastric) ( 4 )

Downstream Regulation
Effects of modification on HSP90B:	intracellular localization ( 1 ) , molecular association, regulation ( 1 )
Effects of modification on biological processes:	cell growth, induced ( 1 ) , transcription, induced ( 1 )
Inhibit interaction with:	AR (human) ( 1 )

References
1	Dagar M, et al. (2019) Phosphorylation of HSP90 by protein kinase A is essential for the nuclear translocation of androgen receptor. J Biol Chem 294, 8699-8710 30992362 Curated Info
2	Schweppe DK, Rigas JR, Gerber SA (2013) Quantitative phosphoproteomic profiling of human non-small cell lung cancer tumors. J Proteomics 91, 286-96 23911959 Curated Info
3	Deb TB, et al. (2011) Pnck induces ligand-independent EGFR degradation by probable perturbation of the Hsp90 chaperone complex. Am J Physiol Cell Physiol 300, C1139-54 21325639 Curated Info
4	Moritz A (2010) CST Curation Set: 9235; Year: 2010; Biosample/Treatment: cell line, MKN-45/calyculin_A & pervanadate; Disease: gastric carcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY]) Curated Info