Thr24
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Home > Phosphorylation Site Page: > Thr24  -  SRC-3 (human)

Site Information
KRKLPCDtPGQGLtC   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 454110

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 6 ) , mass spectrometry ( 4 ) , mutation of modification site ( 1 , 5 , 6 ) , phospho-antibody ( 2 , 3 , 5 , 6 ) , western blotting ( 2 , 3 , 5 , 6 )
Disease tissue studied:
breast cancer ( 1 , 2 , 3 , 5 ) , breast ductal carcinoma ( 1 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 6 ) , BT-483 (breast cell) ( 1 ) , HCC1428 (breast cell) ( 1 ) , HEK293T (epithelial) ( 5 ) , HeLa (cervical) ( 4 , 6 ) , MCF-7 (breast cell) ( 1 , 3 ) , MCF10A1 (epithelial) ( 1 ) , MCF12A (epithelial) ( 1 ) , MEF (fibroblast) [SRC-3 (human)] ( 6 ) , SKBr3 (breast cell) ( 2 ) , T47D (breast cell) ( 1 ) , ZR-75-1 (breast cell) ( 5 )

Upstream Regulation
Regulatory protein:
ER-alpha (human) ( 6 ) , HER2 (human) ( 2 )
Kinases, in vitro:
JNK1 (human) ( 6 ) , NLK (human) ( 1 )
Putative upstream phosphatases:
PDXP (human) ( 5 ) , PPP1CA (human) ( 5 ) , PPP2CA (human) ( 5 )
Treatments:
ATP ( 3 ) , estradiol ( 5 , 6 ) , NU7441 ( 3 ) , siRNA ( 6 )

Downstream Regulation
Effects of modification on SRC-3:
molecular association, regulation ( 6 )
Effects of modification on biological processes:
carcinogenesis, induced ( 1 ) , cell growth, induced ( 1 ) , transcription, induced ( 1 , 3 )
Inhibit interaction with:
ER-alpha (human) ( 6 )

References 

1

Wang X, et al. (2021) Therapeutic targeting of nemo-like kinase in primary and acquired endocrine-resistant breast cancer. Clin Cancer Res
33542078   Curated Info

2

Nikolai BC, et al. (2016) HER2 Signaling Drives DNA Anabolism and Proliferation through SRC-3 Phosphorylation and E2F1-Regulated Genes. Cancer Res 76, 1463-75
26833126   Curated Info

3

Foulds CE, et al. (2013) Proteomic Analysis of Coregulators Bound to ERĪ± on DNA and Nucleosomes Reveals Coregulator Dynamics. Mol Cell 51, 185-99
23850489   Curated Info

4

Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5
21712546   Curated Info

5

Li C, et al. (2008) Essential phosphatases and a phospho-degron are critical for regulation of SRC-3/AIB1 coactivator function and turnover. Mol Cell 31, 835-49
18922467   Curated Info

6

Wu RC, et al. (2004) Selective phosphorylations of the SRC-3/AIB1 coactivator integrate genomic reponses to multiple cellular signaling pathways. Mol Cell 15, 937-49
15383283   Curated Info

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