Tyr783
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Home > Phosphorylation Site Page: > Tyr783  -  PLCG1 (mouse)

Site Information
EGRNPGFyVEANPMP   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447667
Associated spectra:  2 CST

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 1 , 13 ) , mass spectrometry ( 1 , 3 , 9 , 10 , 11 , 12 , 14 , 15 , 16 , 17 , 18 , 19 , 23 , 24 , 26 , 27 , 28 , 29 , 30 , 33 , 34 , 35 , 36 , 37 , 38 , 39 ) , mutation of modification site ( 1 , 40 , 41 ) , phospho-antibody ( 1 , 4 , 5 , 6 , 7 , 8 , 20 , 21 , 22 , 25 , 31 , 40 , 41 , 42 , 43 ) , western blotting ( 1 , 4 , 5 , 6 , 7 , 8 , 13 , 20 , 21 , 22 , 25 , 31 , 42 , 43 )
Disease tissue studied:
testicular cancer ( 9 , 11 )
Relevant cell line - cell type - tissue:
'brain, cerebral cortex' [PCDHGA1 (mouse), homozygous knockout] ( 4 ) , 'stem, embryonic' ( 14 , 15 , 16 , 17 , 18 ) , 'stem, mesenchymal' ( 27 , 28 ) , 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout] ( 40 ) , 3T3 (fibroblast) ( 37 , 38 , 39 ) , BaF3 ('B lymphocyte, precursor') ( 33 , 34 , 35 , 36 ) , C2C12-D (muscle cell) ( 29 ) , endothelial-pulmonary artery ( 7 ) , endothelial-vein ( 7 ) , F9 (testicular) ( 9 ) , Hepa 1-6 (epithelial) ( 23 ) , Jurkat (T lymphocyte) ( 13 ) , mast-bone marrow [SHP-2 (mouse), homozygous knockout] ( 20 ) , MEF (fibroblast) ( 1 , 24 , 30 ) , MEF (fibroblast) [IGF1R (mouse)] ( 41 ) , MEF (fibroblast) [PDGFRA (mouse), genetic knockin] ( 22 ) , MEF (fibroblast) [PTP1B (mouse), homozygous knockout] ( 24 ) , MEF (fibroblast) [Src (mouse)] ( 26 ) , microvessel endothelial [CBL (mouse), homozygous knockout] ( 5 ) , muscle ( 19 ) , P19 (testicular) ( 11 ) , platelet-blood ( 43 ) , PYS-2 (epithelial) ( 12 ) , SF9 ( 42 ) , SYF (fibroblast) [Src (mouse), transfection] ( 3 ) , T lymphocyte ( 25 ) , T lymphocyte-blood ( 21 ) , T lymphocyte-spleen ( 6 , 31 ) , thymocyte [CBL (mouse), homozygous knockout] ( 8 )

Upstream Regulation
Regulatory protein:
CBL (mouse) ( 5 ) , EAR-2 (mouse) ( 25 ) , GIPC1 (mouse) ( 7 ) , LAB (mouse) ( 31 ) , PCDHGA1 (mouse) ( 4 ) , PDGFRA (mouse) ( 22 ) , PTP1B (mouse) ( 24 ) , SHP-2 (mouse) ( 20 )
Putative in vivo kinases:
ITK (human) ( 42 ) , Src (mouse) ( 43 )
Kinases, in vitro:
VEGFR1 (mouse) ( 1 )
Treatments:
anti-CD28 ( 21 ) , anti-CD3 ( 8 , 13 , 32 ) , anti-CD3/CD28 ( 6 , 8 , 31 ) , anti-CD4 ( 8 ) , anti-IgE ( 20 ) , anti-ITGAL ( 21 ) , collagen ( 43 ) , CRP ( 43 ) , EGF ( 24 ) , PDGF ( 22 , 24 , 40 ) , PP2 ( 43 ) , QL9_peptide ( 21 ) , vanadate ( 40 ) , VEGF ( 5 , 7 ) , wortmannin ( 43 )

Downstream Regulation
Effects of modification on PLCG1:
molecular association, regulation ( 1 , 13 , 40 ) , protein conformation ( 1 )
Effects of modification on biological processes:
cell adhesion, altered ( 41 )
Induce interaction with:
PLCG1 (mouse) ( 1 , 40 )

References 

1

Huang Z, et al. (2016) Two FGF Receptor Kinase Molecules Act in Concert to Recruit and Transphosphorylate Phospholipase Cγ. Mol Cell 61, 98-110
26687682   Curated Info

2

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

3

Ferrando IM, et al. (2012) Identification of targets of c-Src tyrosine kinase by chemical complementation and phosphoproteomics. Mol Cell Proteomics 11, 355-69
22499769   Curated Info

4

Garrett AM, Schreiner D, Lobas MA, Weiner JA (2012) γ-protocadherins control cortical dendrite arborization by regulating the activity of a FAK/PKC/MARCKS signaling pathway. Neuron 74, 269-76
22542181   Curated Info

5

Meyer RD, Husain D, Rahimi N (2011) c-Cbl inhibits angiogenesis and tumor growth by suppressing activation of PLCγ1. Oncogene 30, 2198-206
21242968   Curated Info

6

Driessens G, et al. (2011) Beta-catenin inhibits T cell activation by selective interference with linker for activation of T cells-phospholipase C-γ1 phosphorylation. J Immunol 186, 784-90
21149602   Curated Info

7

Lanahan AA, et al. (2010) VEGF receptor 2 endocytic trafficking regulates arterial morphogenesis. Dev Cell 18, 713-24
20434959   Curated Info

8

Thien CB, et al. (2010) c-Cbl promotes T cell receptor-induced thymocyte apoptosis by activating the phosphatidylinositol 3-kinase/Akt pathway. J Biol Chem 285, 10969-81
20133944   Curated Info

9

Zhou J (2010) CST Curation Set: 9105; Year: 2010; Biosample/Treatment: cell line, F9/untreated; Disease: testicular cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-102) Cat#: 9416
Curated Info

10

Zhou J (2010) CST Curation Set: 9104; Year: 2010; Biosample/Treatment: cell line, ES J1/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-102) Cat#: 9416
Curated Info

11

Zhou J (2010) CST Curation Set: 9109; Year: 2010; Biosample/Treatment: cell line, P19/untreated; Disease: testicular cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-102) Cat#: 9416
Curated Info

12

Zhou J (2010) CST Curation Set: 9108; Year: 2010; Biosample/Treatment: cell line, PY2/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-102) Cat#: 9416
Curated Info

13

Carrizosa E, et al. (2009) Hematopoietic lineage cell-specific protein 1 is recruited to the immunological synapse by IL-2-inducible T cell kinase and regulates phospholipase Cgamma1 Microcluster dynamics during T cell spreading. J Immunol 183, 7352-61
19917685   Curated Info

14

Tucker M (2009) CST Curation Set: 8089; Year: 2009; Biosample/Treatment: cell line, embryonic stem cells (mouse)(PTEN)/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

15

Tucker M (2009) CST Curation Set: 8081; Year: 2009; Biosample/Treatment: cell line, embryonic stem cells (mouse)(PTEN)/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

16

Tucker M (2009) CST Curation Set: 8085; Year: 2009; Biosample/Treatment: cell line, embryonic stem cells (mouse)(PTEN)/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

17

Tucker M (2009) CST Curation Set: 8080; Year: 2009; Biosample/Treatment: cell line, embryonic stem cells (mouse)(PTEN)/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

18

Tucker M (2009) CST Curation Set: 8072; Year: 2009; Biosample/Treatment: cell line, embryonic stem cells (mouse)(PTEN)/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY] Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine (P-Tyr-1000) Rabbit mAb Cat#: 8954, Phospho-Tyrosine Rabbit mAb (p-Tyr-1000) Immunoaffinity Beads Cat#: 8876
Curated Info

19

Guo A (2009) CST Curation Set: 8162; Year: 2009; Biosample/Treatment: tissue, muscle/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: (F/Y/M)Xp[ST](L/I/M)
Curated Info

20

McPherson VA, et al. (2009) SH2 domain-containing phosphatase-2 protein-tyrosine phosphatase promotes Fc(epsilon)RI-induced activation of Fyn and Erk pathways leading to TNF(alpha) release from bone marrow-derived mast cells. J Immunol 183, 4940-7
19786542   Curated Info

21

Kim K, Wang L, Hwang I (2009) LFA-1-dependent Ca2+ entry following suboptimal T cell receptor triggering proceeds without mobilization of intracellular Ca2+. J Biol Chem 284, 22149-54
19542227   Curated Info

22

Olson LE, Soriano P (2009) Increased PDGFRalpha activation disrupts connective tissue development and drives systemic fibrosis. Dev Cell 16, 303-13
19217431   Curated Info

23

Pan C, Gnad F, Olsen JV, Mann M (2008) Quantitative phosphoproteome analysis of a mouse liver cell line reveals specificity of phosphatase inhibitors. Proteomics 8, 4534-46
18846507   Curated Info

24

Mertins P, et al. (2008) Investigation of protein-tyrosine phosphatase 1B function by quantitative proteomics. Mol Cell Proteomics 7, 1763-77
18515860   Curated Info

25

Hermann-Kleiter N, et al. (2008) The nuclear orphan receptor NR2F6 suppresses lymphocyte activation and T helper 17-dependent autoimmunity. Immunity 29, 205-16
18701084   Curated Info

26

Luo W, et al. (2008) Global impact of oncogenic Src on a phosphotyrosine proteome. J Proteome Res 7, 3447-60
18563927   Curated Info

27

Yu J (2007) CST Curation Set: 3379; Year: 2007; Biosample/Treatment: cell line, Mouse Mesenchymal Stem Cells/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

28

Yu J (2007) CST Curation Set: 3380; Year: 2007; Biosample/Treatment: cell line, Mouse Mesenchymal Stem Cells/bFGF; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

29

Guo A (2007) CST Curation Set: 2564; Year: 2007; Biosample/Treatment: cell line, C2C12-D/-; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

30

Li Y (2006) CST Curation Set: 2051; Year: 2006; Biosample/Treatment: cell line, MEF/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

31

Zhu M, et al. (2006) Negative regulation of T cell activation and autoimmunity by the transmembrane adaptor protein LAB. Immunity 25, 757-68
17081783   Curated Info

32

Jordan MS, et al. (2006) Functional hierarchy of the N-terminal tyrosines of SLP-76. J Immunol 176, 2430-8
16456002   Curated Info

33

Gu T (2005) CST Curation Set: 844; Year: 2005; Biosample/Treatment: cell line, BaF3/-; Disease: chronic myelogenous leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

34

Gu T (2005) CST Curation Set: 845; Year: 2005; Biosample/Treatment: cell line, BaF3/-; Disease: chronic myelogenous leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

35

Gu T (2005) CST Curation Set: 846; Year: 2005; Biosample/Treatment: cell line, BaF3/-; Disease: chronic myelogenous leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

36

Gu T (2005) CST Curation Set: 699; Year: 2005; Biosample/Treatment: cell line, BaF3/-; Disease: chronic myelogenous leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

37

Guo A (2005) CST Curation Set: 630; Year: 2005; Biosample/Treatment: cell line, 3T3/EGF; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

38

Guo A (2005) CST Curation Set: 632; Year: 2005; Biosample/Treatment: cell line, 3T3/EGF; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

39

Guo A (2005) CST Curation Set: 634; Year: 2005; Biosample/Treatment: cell line, 3T3/EGF; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

40

Poulin B, Sekiya F, Rhee SG (2005) Intramolecular interaction between phosphorylated tyrosine-783 and the C-terminal Src homology 2 domain activates phospholipase C-gamma1. Proc Natl Acad Sci U S A 102, 4276-81
15764700   Curated Info

41

Tvorogov D, Wang XJ, Zent R, Carpenter G (2005) Integrin-dependent PLC-gamma1 phosphorylation mediates fibronectin-dependent adhesion. J Cell Sci 118, 601-10
15657076   Curated Info

42

Wilcox HM, Berg LJ (2003) Itk phosphorylation sites are required for functional activity in primary T cells. J Biol Chem 278, 37112-21
12842872   Curated Info

43

Suzuki-Inoue K, Inoue O, Frampton J, Watson SP (2003) Murine GPVI stimulates weak integrin activation in PLCgamma2-/- platelets: involvement of PLCgamma1 and PI3-kinase. Blood 102, 1367-73
12730118   Curated Info