Thr104
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Home > Phosphorylation Site Page: > Thr104  -  JunB (human)

Site Information
GVITTtPtPPGQyFy   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 1814000

In vivo Characterization
Methods used to characterize site in vivo:
electrophoretic mobility shift ( 10 ) , mass spectrometry ( 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 ) , mutation of modification site ( 10 )
Disease tissue studied:
breast cancer ( 2 ) , HER2 positive breast cancer ( 1 ) , luminal A breast cancer ( 1 ) , luminal B breast cancer ( 1 ) , breast cancer, triple negative ( 1 ) , cervical cancer ( 8 ) , cervical adenocarcinoma ( 8 ) , gastric cancer ( 5 , 6 ) , gastric carcinoma ( 5 , 6 ) , lung cancer ( 4 ) , non-small cell lung cancer ( 4 ) , lymphoma ( 10 ) , B cell lymphoma ( 10 )
Relevant cell line - cell type - tissue:
breast ( 1 ) , Calu 6 (pulmonary) ( 4 ) , HeLa (cervical) ( 7 , 9 ) , HeLa S3 (cervical) ( 8 ) , HMLER ('stem, breast cancer') ( 2 ) , HMLER ('stem, breast cancer') [CXCR4 (human), knockdown] ( 2 ) , Jurkat (T lymphocyte) ( 3 ) , M12 (B lymphocyte) ( 10 ) , MKN-45 (gastric) ( 5 , 6 ) , NCI-H1648 (pulmonary) ( 4 ) , T lymphocyte-lymph node ( 10 )

Upstream Regulation
Putative in vivo kinases:
JNK1 (mouse) ( 10 )
Kinases, in vitro:
JNK1 (human) ( 10 )
Treatments:
ionomycin ( 10 ) , nocodazole ( 8 ) , phorbol_ester ( 10 )

Downstream Regulation
Effects of modification on JunB:
activity, induced ( 10 ) , molecular association, regulation ( 10 )
Effects of modification on biological processes:
transcription, altered ( 10 )
Induce interaction with:
DNA ( 10 )

References 

1

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

2

Yi T, et al. (2014) Quantitative phosphoproteomic analysis reveals system-wide signaling pathways downstream of SDF-1/CXCR4 in breast cancer stem cells. Proc Natl Acad Sci U S A 111, E2182-90
24782546   Curated Info

3

Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7
23749302   Curated Info

4

Klammer M, et al. (2012) Phosphosignature predicts dasatinib response in non-small cell lung cancer. Mol Cell Proteomics 11, 651-68
22617229   Curated Info

5

Moritz A (2011) CST Curation Set: 12383; Year: 2011; Biosample/Treatment: cell line, MKN-45/untreated; Disease: gastric carcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P , PTMScan(R) PLK Binding Motif (SpTP) Immunoaffinity Beads Cat#: 1995
Curated Info

6

Moritz A (2011) CST Curation Set: 12386; Year: 2011; Biosample/Treatment: cell line, MKN-45/untreated; Disease: gastric carcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P , PTMScan(R) PLK Binding Motif (SpTP) Immunoaffinity Beads Cat#: 1995
Curated Info

7

Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5
21712546   Curated Info

8

Olsen JV, et al. (2010) Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal 3, ra3
20068231   Curated Info

9

Dephoure N, et al. (2008) A quantitative atlas of mitotic phosphorylation. Proc Natl Acad Sci U S A 105, 10762-7
18669648   Curated Info

10

Li B, Tournier C, Davis RJ, Flavell RA (1999) Regulation of IL-4 expression by the transcription factor JunB during T helper cell differentiation. EMBO J 18, 420-32
9889198   Curated Info