Ser177
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Home > Phosphorylation Site Page: > Ser177  -  PARP1 (human)

Site Information
LGFRPEYsAsQLkGF   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 18013484

In vivo Characterization
Methods used to characterize site in vivo:
[32P] ATP in vitro ( 3 ) , immunoassay ( 3 ) , mass spectrometry ( 1 , 4 , 5 ) , mutation of modification site ( 3 )
Disease tissue studied:
cervical cancer ( 5 ) , cervical adenocarcinoma ( 5 )
Relevant cell line - cell type - tissue:
HeLa (cervical) ( 1 ) , HeLa S3 (cervical) ( 5 ) , HUVEC (endothelial) ( 3 ) , K562 (erythroid) ( 4 ) , MEF (fibroblast) ( 3 )

Upstream Regulation
Putative in vivo kinases:
AMPKA2 (human) ( 3 )
Kinases, in vitro:
AMPKA1 (human) ( 3 )
Treatments:
acadesine ( 3 ) , compound_C ( 3 ) , metformin ( 3 ) , siRNA ( 3 )

Downstream Regulation
Effects of modification on PARP1:
molecular association, regulation ( 3 )
Effects of modification on biological processes:
cell adhesion, induced ( 3 ) , signaling pathway regulation ( 3 ) , transcription, altered ( 3 )
Inhibit interaction with:
DNA ( 3 )

References 

1

Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94
25159151   Curated Info

2

Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21
23312004   Curated Info

3

Gongol B, et al. (2013) AMPK╬▒2 exerts its anti-inflammatory effects through PARP-1 and Bcl-6. Proc Natl Acad Sci U S A 110, 3161-6
23382195   Curated Info

4

Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71
23186163   Curated Info

5

Olsen JV, et al. (2010) Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal 3, ra3
20068231   Curated Info