Ser473
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Home > Phosphorylation Site Page: > Ser473  -  TIF1B (mouse)

Site Information
SGMkRsRsGEGEVSG   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 452391
Associated spectra:  1 CST

In vivo Characterization
Methods used to characterize site in vivo:
flow cytometry ( 2 , 3 ) , immunoassay ( 24 ) , immunoprecipitation ( 3 , 10 , 51 ) , mass spectrometry ( 3 , 4 , 5 , 7 , 8 , 9 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 19 , 20 , 21 , 22 , 23 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 52 , 53 , 54 , 55 , 56 , 57 ) , mutation of modification site ( 3 , 10 ) , phospho-antibody ( 2 , 3 , 10 , 18 , 24 , 51 ) , western blotting ( 2 , 3 , 10 , 18 , 24 , 51 )
Disease tissue studied:
anthrax infection ( 29 ) , bone cancer ( 24 ) , leukemia ( 21 , 51 ) , acute myelogenous leukemia ( 21 ) , chronic myelogenous leukemia ( 51 ) , lung cancer ( 24 ) , non-small cell lung cancer ( 24 ) , lymphoma ( 57 ) , B cell lymphoma ( 57 ) , neuroblastoma ( 27 ) , melanoma skin cancer ( 41 ) , testicular cancer ( 35 )
Relevant cell line - cell type - tissue:
'3T3-L1, differentiated' (adipocyte) ( 7 , 9 , 15 ) , 'brain, cerebral cortex' ( 38 ) , 'fat, brown' ( 32 ) , 293 (epithelial) ( 24 ) , 32Dcl3 (myeloid) [FLT3 (mouse), transfection, chimera with human FLT3-ITD mutant (corresponding to wild type P36888 ( 39 ) , 32Dcl3 (myeloid) ( 39 ) , 3T3 (fibroblast) ( 50 , 55 ) , BaF3 ('B lymphocyte, precursor') [JAK3 (human), transfection] ( 4 ) , blood ( 21 ) , brain ( 22 , 23 , 32 , 34 , 56 ) , C2C12 (myoblast) ( 10 , 43 , 44 , 45 , 46 , 47 , 48 , 49 ) , colon ( 3 ) , dendritic cell ( 2 ) , ES-J1 (stem) ( 36 ) , heart ( 16 , 30 , 32 ) , HEK293T (epithelial) ( 51 ) , HeLa (cervical) ( 31 , 51 ) , Hepa 1-6 (epithelial) ( 42 ) , HL-1 (myocyte) [Akt1 (mouse), knockdown, stable lentiviral expression of Akt1 shRNA] ( 12 ) , HL-1 (myocyte) [Akt2 (mouse), knockdown, stable lentiviral expression of Akt2 shRNA] ( 12 ) , HL-1 (myocyte) ( 12 ) , K562 (erythroid) ( 51 ) , kidney ( 32 ) , liver ( 5 , 14 , 20 , 32 , 52 , 54 ) , liver [leptin (mouse), homozygous knockout] ( 20 ) , lung ( 32 ) , lymph node ( 2 ) , macrophage-bone marrow ( 33 ) , macrophage-bone marrow [DUSP1 (mouse), homozygous knockout] ( 33 ) , macrophage-peritoneum ( 19 ) , MC3T3-E1 (preosteoblast) ( 8 ) , MEF (fibroblast) ( 17 , 24 , 26 ) , MEF (fibroblast) [p53 (mouse), homozygous knockout] ( 25 ) , MEF (fibroblast) [Raptor (mouse), knockdown] ( 17 ) , MEF (fibroblast) [RICTOR (mouse), knockdown] ( 17 ) , MEF (fibroblast) [TSC2 (mouse), homozygous knockout] ( 26 ) , mpkCCD (renal) ( 37 ) , N1E-115 (neuron) ( 27 ) , NCI-H1299 (pulmonary) ( 24 ) , P19 (testicular) ( 35 , 53 ) , pancreas ( 32 ) , RAW 264.7 (macrophage) ( 11 ) , RAW 267.4 (macrophage) ( 40 ) , skin [mGluR1 (mouse), transgenic, TG mutant mice] ( 41 ) , spleen ( 2 , 29 , 32 ) , stromal ( 13 ) , T lymphocyte ( 3 ) , T lymphocyte-spleen ( 28 ) , testis ( 32 ) , thymocyte ( 18 ) , thymus ( 2 ) , U2OS (bone cell) ( 24 ) , WEHI-231 (B lymphocyte) ( 57 )

Upstream Regulation
Regulatory protein:
ATM (human) ( 24 ) , DNAPK (human) ( 24 ) , HP1 alpha (human) ( 24 ) , Raptor (mouse) ( 17 ) , RICTOR (mouse) ( 17 )
Putative in vivo kinases:
MSK1 (human) ( 10 ) , PKCD (human) ( 51 )
Kinases, in vitro:
MSK1 (human) ( 10 )
Treatments:
anti-CD28 ( 18 ) , anti-CD3 ( 18 ) , D4476 ( 51 ) , development ( 2 ) , insulin ( 15 ) , ionizing_radiation ( 24 ) , ischemia ( 13 ) , KN-93 ( 51 ) , LCMV_gp33-41_peptide ( 28 ) , LPS ( 33 ) , NAG-thiazoline ( 50 ) , neocarzinostatin ( 24 ) , nocodazole ( 51 ) , phorbol_ester ( 51 ) , PUGNAc ( 50 ) , Ro31-8220 ( 51 ) , serum ( 51 ) , staurosporine ( 51 )

Downstream Regulation
Effects of modification on TIF1B:
intracellular localization ( 51 ) , molecular association, regulation ( 10 , 51 )
Effects of modification on biological processes:
cell cycle regulation ( 51 ) , cell differentiation, induced ( 10 ) , chromatin organization, altered ( 2 ) , DNA repair, induced ( 24 ) , transcription, altered ( 51 ) , transcription, induced ( 10 ) , transcription, inhibited ( 2 )
Inhibit interaction with:
CBX1 (human) ( 51 ) , EHMT2 (mouse) ( 10 ) , HDAC11 (mouse) ( 10 )

Disease / Diagnostics Relevance
Relevant diseases:
colonic inflamation ( 3 )

References 

1

Steinert ND, et al. (2021) Mapping of the contraction-induced phosphoproteome identifies TRIM28 as a significant regulator of skeletal muscle size and function. Cell Rep 34, 108796
33657380   Curated Info

2

Chikuma S, et al. (2021) TRIM28 Expression on Dendritic Cells Prevents Excessive T Cell Priming by Silencing Endogenous Retrovirus. J Immunol
33619215   Curated Info

3

Lyons J, et al. (2018) Integrated in vivo multiomics analysis identifies p21-activated kinase signaling as a driver of colitis. Sci Signal 11
29487189   Curated Info

4

Degryse S, et al. (2017) Mutant JAK3 phosphoproteomic profiling predicts synergism between JAK3 inhibitors and MEK/BCL2 inhibitors for the treatment of T-cell acute lymphoblastic leukemia. Leukemia
28852199   Curated Info

5

Robles MS, Humphrey SJ, Mann M (2017) Phosphorylation Is a Central Mechanism for Circadian Control of Metabolism and Physiology. Cell Metab 25, 118-127
27818261   Curated Info

6

Sacco F, et al. (2016) Glucose-regulated and drug-perturbed phosphoproteome reveals molecular mechanisms controlling insulin secretion. Nat Commun 7, 13250
27841257   Curated Info

7

Minard AY, et al. (2016) mTORC1 Is a Major Regulatory Node in the FGF21 Signaling Network in Adipocytes. Cell Rep 17, 29-36
27681418   Curated Info

8

Williams GR, et al. (2016) Exploring G protein-coupled receptor signaling networks using SILAC-based phosphoproteomics. Methods 92, 36-50
26160508   Curated Info

9

Parker BL, et al. (2015) Targeted phosphoproteomics of insulin signaling using data-independent acquisition mass spectrometry. Sci Signal 8, rs6
26060331   Curated Info

10

Singh K, et al. (2015) A KAP1 phosphorylation switch controls MyoD function during skeletal muscle differentiation. Genes Dev 29, 513-25
25737281   Curated Info

11

Pinto SM, et al. (2015) Quantitative phosphoproteomic analysis of IL-33-mediated signaling. Proteomics 15, 532-44
25367039   Curated Info

12

Reinartz M, Raupach A, Kaisers W, Gödecke A (2014) AKT1 and AKT2 induce distinct phosphorylation patterns in HL-1 cardiac myocytes. J Proteome Res 13, 4232-45
25162660   Curated Info

13

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

14

Wilson-Grady JT, Haas W, Gygi SP (2013) Quantitative comparison of the fasted and re-fed mouse liver phosphoproteomes using lower pH reductive dimethylation. Methods 61, 277-86
23567750   Curated Info

15

Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2. Cell Metab 17, 1009-20
23684622   Curated Info

16

Lundby A, et al. (2013) In vivo phosphoproteomics analysis reveals the cardiac targets of β-adrenergic receptor signaling. Sci Signal 6, rs11
23737553   Curated Info

17

Robitaille AM, et al. (2013) Quantitative phosphoproteomics reveal mTORC1 activates de novo pyrimidine synthesis. Science 339, 1320-3
23429704   Curated Info

18

Zhou XF, et al. (2012) TRIM28 mediates chromatin modifications at the TCRα enhancer and regulates the development of T and natural killer T cells. Proc Natl Acad Sci U S A 109, 20083-8
23169648   Curated Info

19

Wu X, et al. (2012) Investigation of receptor interacting protein (RIP3)-dependent protein phosphorylation by quantitative phosphoproteomics. Mol Cell Proteomics 11, 1640-51
22942356   Curated Info

20

Grimsrud PA, et al. (2012) A quantitative map of the liver mitochondrial phosphoproteome reveals posttranslational control of ketogenesis. Cell Metab 16, 672-83
23140645   Curated Info

21

Trost M, et al. (2012) Posttranslational regulation of self-renewal capacity: insights from proteome and phosphoproteome analyses of stem cell leukemia. Blood 120, e17-27
22802335   Curated Info

22

Trinidad JC, et al. (2012) Global identification and characterization of both O-GlcNAcylation and phosphorylation at the murine synapse. Mol Cell Proteomics 11, 215-29
22645316   Curated Info

23

Goswami T, et al. (2012) Comparative phosphoproteomic analysis of neonatal and adult murine brain. Proteomics 12, 2185-9
22807455   Curated Info

24

White D, et al. (2012) The ATM substrate KAP1 controls DNA repair in heterochromatin: regulation by HP1 proteins and serine 473/824 phosphorylation. Mol Cancer Res 10, 401-14
22205726   Curated Info

25

Hsu PP, et al. (2011) The mTOR-regulated phosphoproteome reveals a mechanism of mTORC1-mediated inhibition of growth factor signaling. Science 332, 1317-22
21659604   Curated Info

26

Yu Y, et al. (2011) Phosphoproteomic analysis identifies Grb10 as an mTORC1 substrate that negatively regulates insulin signaling. Science 332, 1322-6
21659605   Curated Info

27

Wang Y, et al. (2011) Spatial phosphoprotein profiling reveals a compartmentalized extracellular signal-regulated kinase switch governing neurite growth and retraction. J Biol Chem 286, 18190-201
21454597   Curated Info

28

Navarro MN, et al. (2011) Phosphoproteomic analysis reveals an intrinsic pathway for the regulation of histone deacetylase 7 that controls the function of cytotoxic T lymphocytes. Nat Immunol 12, 352-61
21399638   Curated Info

29

Manes NP, et al. (2011) Discovery of mouse spleen signaling responses to anthrax using label-free quantitative phosphoproteomics via mass spectrometry. Mol Cell Proteomics 10, M110.000927
21189417   Curated Info

30

Deng N, et al. (2011) Phosphoproteome analysis reveals regulatory sites in major pathways of cardiac mitochondria. Mol Cell Proteomics 10, M110.000117
20495213   Curated Info

31

Hegemann B, et al. (2011) Systematic phosphorylation analysis of human mitotic protein complexes. Sci Signal 4, rs12
22067460   Curated Info

32

Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89
21183079   Curated Info

33

Weintz G, et al. (2010) The phosphoproteome of toll-like receptor-activated macrophages. Mol Syst Biol 6, 371
20531401   Curated Info

34

Wiśniewski JR, et al. (2010) Brain phosphoproteome obtained by a FASP-based method reveals plasma membrane protein topology. J Proteome Res 9, 3280-9
20415495   Curated Info

35

Zhou J (2010) CST Curation Set: 9674; Year: 2010; Biosample/Treatment: cell line, P19/untreated; Disease: testicular cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) PKD Substrate Antibody Cat#: 4381, PTMScan(R) Phospho-PKD Substrate Motif (LXRXXpS/pT) Immunoaffinity Beads Cat#: 1986
Curated Info

36

Zhou J (2010) CST Curation Set: 9670; Year: 2010; Biosample/Treatment: cell line, ES J1/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) PKD Substrate Antibody Cat#: 4381, PTMScan(R) Phospho-PKD Substrate Motif (LXRXXpS/pT) Immunoaffinity Beads Cat#: 1986
Curated Info

37

Rinschen MM, et al. (2010) Quantitative phosphoproteomic analysis reveals vasopressin V2-receptor-dependent signaling pathways in renal collecting duct cells. Proc Natl Acad Sci U S A 107, 3882-7
20139300   Curated Info

38

Tweedie-Cullen RY, Reck JM, Mansuy IM (2009) Comprehensive mapping of post-translational modifications on synaptic, nuclear, and histone proteins in the adult mouse brain. J Proteome Res 8, 4966-82
19737024   Curated Info

39

Choudhary C, et al. (2009) Mislocalized activation of oncogenic RTKs switches downstream signaling outcomes. Mol Cell 36, 326-39
19854140   Curated Info

40

Trost M, et al. (2009) The phagosomal proteome in interferon-gamma-activated macrophages. Immunity 30, 143-54
19144319   Curated Info

41

Zanivan S, et al. (2008) Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry. J Proteome Res 7, 5314-26
19367708   Curated Info

42

Pan C, Gnad F, Olsen JV, Mann M (2008) Quantitative phosphoproteome analysis of a mouse liver cell line reveals specificity of phosphatase inhibitors. Proteomics 8, 4534-46
18846507   Curated Info

43

Possemato A (2008) CST Curation Set: 5251; Year: 2008; Biosample/Treatment: cell line, C2C12/serum starved; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST]
Curated Info

44

Possemato A (2008) CST Curation Set: 5252; Year: 2008; Biosample/Treatment: cell line, C2C12/serum starved; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST]
Curated Info

45

Possemato A (2008) CST Curation Set: 5253; Year: 2008; Biosample/Treatment: cell line, C2C12/serum starved; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST]
Curated Info

46

Possemato A (2008) CST Curation Set: 5254; Year: 2008; Biosample/Treatment: cell line, C2C12/serum starved; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST]
Curated Info

47

Possemato A (2008) CST Curation Set: 5255; Year: 2008; Biosample/Treatment: cell line, C2C12/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST]
Curated Info

48

Possemato A (2008) CST Curation Set: 5256; Year: 2008; Biosample/Treatment: cell line, C2C12/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST]
Curated Info

49

Possemato A (2008) CST Curation Set: 5233; Year: 2008; Biosample/Treatment: cell line, C2C12/serum starved; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST]
Curated Info

50

Wang Z, Gucek M, Hart GW (2008) Cross-talk between GlcNAcylation and phosphorylation: site-specific phosphorylation dynamics in response to globally elevated O-GlcNAc. Proc Natl Acad Sci U S A 105, 13793-8
18779572   Curated Info

51

Chang CW, et al. (2008) Phosphorylation at Ser473 regulates heterochromatin protein 1 binding and corepressor function of TIF1beta/KAP1. BMC Mol Biol 9, 61
18590578   Curated Info

52

Possemato A (2007) CST Curation Set: 3395; Year: 2007; Biosample/Treatment: tissue, liver/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) PKD Substrate Antibody Cat#: 4381, PTMScan(R) Phospho-PKD Substrate Motif (LXRXXpS/pT) Immunoaffinity Beads Cat#: 1986
Curated Info

53

Smith JC, et al. (2007) A differential phosphoproteomic analysis of retinoic acid-treated P19 cells. J Proteome Res 6, 3174-86
17622165   Curated Info

54

Villén J, Beausoleil SA, Gerber SA, Gygi SP (2007) Large-scale phosphorylation analysis of mouse liver. Proc Natl Acad Sci U S A 104, 1488-93
17242355   Curated Info

55

Farnsworth C (2006) CST Curation Set: 1141; Year: 2006; Biosample/Treatment: cell line, 3T3/0.5% serum &'||' TPA; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Ser/Thr) PKD Substrate Antibody Cat#: 4381, PTMScan(R) Phospho-PKD Substrate Motif (LXRXXpS/pT) Immunoaffinity Beads Cat#: 1986
Curated Info

56

Ballif BA, et al. (2004) Phosphoproteomic analysis of the developing mouse brain. Mol Cell Proteomics 3, 1093-101
15345747   Curated Info

57

Shu H, et al. (2004) Identification of phosphoproteins and their phosphorylation sites in the WEHI-231 B lymphoma cell line. Mol Cell Proteomics 3, 279-86
14729942   Curated Info