Ser428
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Home > Phosphorylation Site Page: > Ser428  -  MAD1L1 (human)

Site Information
ELtPAEYsPQLtRRM   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 453666

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 22 ) , mass spectrometry ( 1 , 2 , 3 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 ) , mutation of modification site ( 22 )
Disease tissue studied:
breast cancer ( 8 ) , breast ductal carcinoma ( 8 ) , HER2 positive breast cancer ( 3 ) , luminal A breast cancer ( 3 ) , luminal B breast cancer ( 3 ) , breast cancer, triple negative ( 3 , 8 ) , cervical cancer ( 19 ) , cervical adenocarcinoma ( 19 ) , leukemia ( 12 ) , acute myelogenous leukemia ( 12 ) , lung cancer ( 6 , 11 , 17 ) , non-small cell lung cancer ( 11 ) , non-small cell lung adenocarcinoma ( 6 ) , ovarian cancer ( 8 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 21 ) , 293E (epithelial) ( 13 ) , breast ( 3 , 8 ) , Calu 6 (pulmonary) ( 11 ) , CL1-0 (pulmonary) ( 17 ) , CL1-1 (pulmonary) ( 17 ) , CL1-2 (pulmonary) ( 17 ) , CL1-5 (pulmonary) ( 17 ) , H2009 (pulmonary) ( 11 ) , H2077 (pulmonary) ( 11 ) , H2887 (pulmonary) ( 11 ) , H322M (pulmonary) ( 11 ) , HCC366 (pulmonary) ( 11 ) , HCC78 (pulmonary) ( 11 ) , HeLa (cervical) ( 2 , 5 , 7 , 10 , 15 , 22 , 24 , 25 , 26 , 27 , 28 , 29 ) , HeLa S3 (cervical) ( 14 , 19 , 23 ) , HeLa_BI (cervical) ( 16 ) , HeLa_Hesp (cervical) ( 16 ) , HeLa_LOG (cervical) ( 16 ) , HeLa_Meta (cervical) ( 18 ) , HeLa_NOC (cervical) ( 16 ) , HeLa_Pro (cervical) ( 18 ) , HeLa_Telo (cervical) ( 18 ) , HOP62 (pulmonary) ( 11 ) , HUES-7 ('stem, embryonic') ( 20 ) , Jurkat (T lymphocyte) ( 9 ) , K562 (erythroid) ( 10 ) , KG-1 (myeloid) ( 12 ) , NCI-H1648 (pulmonary) ( 11 ) , NCI-H322 (pulmonary) ( 11 ) , ovary ( 8 ) , PC9 (pulmonary) ( 6 ) , Vero E6-S ('epithelial, kidney') ( 1 )

Upstream Regulation
Treatments:
metastatic potential ( 17 ) , MG132 ( 5 ) , MG132_withdrawal ( 18 ) , nocodazole ( 5 , 19 ) , thymidine ( 5 )

References 

1

Bouhaddou M, et al. (2020) The Global Phosphorylation Landscape of SARS-CoV-2 Infection. Cell
32645325   Curated Info

2

Huang H, et al. (2016) Simultaneous Enrichment of Cysteine-containing Peptides and Phosphopeptides Using a Cysteine-specific Phosphonate Adaptable Tag (CysPAT) in Combination with titanium dioxide (TiO2) Chromatography. Mol Cell Proteomics 15, 3282-3296
27281782   Curated Info

3

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

4

Boeing S, et al. (2016) Multiomic Analysis of the UV-Induced DNA Damage Response. Cell Rep 15, 1597-1610
27184836   Curated Info

5

Bauer M, et al. (2015) Assessment of current mass spectrometric workflows for the quantification of low abundant proteins and phosphorylation sites. Data Brief 5, 297-304
26550600   Curated Info

6

Tsai CF, et al. (2015) Large-scale determination of absolute phosphorylation stoichiometries in human cells by motif-targeting quantitative proteomics. Nat Commun 6, 6622
25814448   Curated Info

7

Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94
25159151   Curated Info

8

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

9

Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7
23749302   Curated Info

10

Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71
23186163   Curated Info

11

Klammer M, et al. (2012) Phosphosignature predicts dasatinib response in non-small cell lung cancer. Mol Cell Proteomics 11, 651-68
22617229   Curated Info

12

Weber C, Schreiber TB, Daub H (2012) Dual phosphoproteomics and chemical proteomics analysis of erlotinib and gefitinib interference in acute myeloid leukemia cells. J Proteomics 75, 1343-56
22115753   Curated Info

13

Hsu PP, et al. (2011) The mTOR-regulated phosphoproteome reveals a mechanism of mTORC1-mediated inhibition of growth factor signaling. Science 332, 1317-22
21659604   Curated Info

14

Santamaria A, et al. (2011) The Plk1-dependent phosphoproteome of the early mitotic spindle. Mol Cell Proteomics 10, M110.004457
20860994   Curated Info

15

Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5
21712546   Curated Info

16

Hegemann B, et al. (2011) Systematic phosphorylation analysis of human mitotic protein complexes. Sci Signal 4, rs12
22067460   Curated Info

17

Wang YT, et al. (2010) An informatics-assisted label-free quantitation strategy that depicts phosphoproteomic profiles in lung cancer cell invasion. J Proteome Res 9, 5582-97
20815410   Curated Info

18

Dulla K, et al. (2010) Quantitative site-specific phosphorylation dynamics of human protein kinases during mitotic progression. Mol Cell Proteomics 9, 1167-81
20097925   Curated Info

19

Olsen JV, et al. (2010) Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal 3, ra3
20068231   Curated Info

20

Van Hoof D, et al. (2009) Phosphorylation dynamics during early differentiation of human embryonic stem cells. Cell Stem Cell 5, 214-26
19664995   Curated Info

21

Gauci S, et al. (2009) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach. Anal Chem 81, 4493-501
19413330   Curated Info

22

Chi YH, et al. (2008) Requirements for protein phosphorylation and the kinase activity of polo-like kinase 1 (Plk1) for the kinetochore function of mitotic arrest deficiency protein 1 (Mad1). J Biol Chem 283, 35834-44
18922800   Curated Info

23

Daub H, et al. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle. Mol Cell 31, 438-48
18691976   Curated Info

24

Dephoure N, et al. (2008) A quantitative atlas of mitotic phosphorylation. Proc Natl Acad Sci U S A 105, 10762-7
18669648   Curated Info

25

Ruse CI, et al. (2008) Motif-specific sampling of phosphoproteomes. J Proteome Res 7, 2140-50
18452278   Curated Info

26

Cantin GT, et al. (2008) Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis. J Proteome Res 7, 1346-51
18220336   Curated Info

27

Beausoleil SA, et al. (2006) A probability-based approach for high-throughput protein phosphorylation analysis and site localization. Nat Biotechnol 24, 1285-92
16964243   Curated Info

28

Nousiainen M, et al. (2006) Phosphoproteome analysis of the human mitotic spindle. Proc Natl Acad Sci U S A 103, 5391-6
16565220   Curated Info

29

Beausoleil SA, et al. (2004) Large-scale characterization of HeLa cell nuclear phosphoproteins. Proc Natl Acad Sci U S A 101, 12130-5
15302935   Curated Info