Ser477
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Home > Phosphorylation Site Page: > Ser477  -  NEDD4L (mouse)

Site Information
IRRPRsLssPtVtLS   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 453440

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 1 , 2 , 4 , 5 , 6 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 ) , mutation of modification site ( 16 , 27 ) , phospho-antibody ( 7 , 16 , 26 , 27 ) , western blotting ( 7 , 16 )
Disease tissue studied:
anthrax infection ( 19 ) , pancreatic cancer ( 7 ) , pancreatic carcinoma ( 7 ) , melanoma skin cancer ( 24 )
Relevant cell line - cell type - tissue:
'3T3-L1, differentiated' (adipocyte) ( 6 , 11 ) , 'brain, cerebral cortex' ( 23 ) , 'brain, striatum' ( 5 ) , 'fat, brown' ( 20 ) , 293 (epithelial) ( 27 ) , BaF3 ('B lymphocyte, precursor') [JAK3 (human), transfection] ( 1 ) , brain ( 20 ) , CFPAC-1 (pancreatic) ( 7 ) , fibroblast-lung ( 21 ) , heart ( 12 ) , HEK293T (epithelial) ( 16 ) , Hepa 1-6 (epithelial) ( 25 ) , HL-1 (myocyte) [Akt1 (mouse), knockdown, stable lentiviral expression of Akt1 shRNA] ( 8 ) , HL-1 (myocyte) [Akt2 (mouse), knockdown, stable lentiviral expression of Akt2 shRNA] ( 8 ) , HL-1 (myocyte) ( 8 ) , kidney ( 20 ) , liver ( 2 , 10 , 14 ) , liver [leptin (mouse), homozygous knockout] ( 14 ) , lung ( 20 ) , macrophage-peritoneum ( 13 ) , MEF (fibroblast) ( 13 ) , MEF (fibroblast) [p53 (mouse), homozygous knockout] ( 17 ) , mpkCCD (renal) ( 16 , 22 , 26 ) , neuron:postsynaptic density-'brain, hippocampus, CA1 region' ( 4 ) , oocyte [CPEB (mouse)] ( 27 ) , pancreas ( 20 ) , PC-12 (chromaffin) [TrkA (rat), transfection] ( 15 ) , PC-12 (chromaffin) ( 15 ) , skin [mGluR1 (mouse), transgenic, TG mutant mice] ( 24 ) , spleen ( 19 , 20 ) , stromal ( 9 ) , T lymphocyte-spleen ( 18 )

Upstream Regulation
Regulatory protein:
SGK1 (human) ( 7 )
Putative in vivo kinases:
SGK1 (human) ( 27 )
Treatments:
aldosterone ( 16 , 27 ) , ceramide ( 7 ) , colforsin ( 5 ) , GSK2334470 ( 7 ) , GSK650394 ( 7 ) , LY294002 ( 27 )

Downstream Regulation
Effects of modification on NEDD4L:
intracellular localization ( 7 ) , molecular association, regulation ( 7 , 16 , 26 ) , protein stabilization ( 16 ) , ubiquitination ( 7 )
Induce interaction with:
14-3-3 beta (human) ( 16 ) , 14-3-3 beta (mouse) ( 26 )
Inhibit interaction with:
CFTR (human) ( 7 )

References 

1

Degryse S, et al. (2017) Mutant JAK3 phosphoproteomic profiling predicts synergism between JAK3 inhibitors and MEK/BCL2 inhibitors for the treatment of T-cell acute lymphoblastic leukemia. Leukemia
28852199   Curated Info

2

Robles MS, Humphrey SJ, Mann M (2017) Phosphorylation Is a Central Mechanism for Circadian Control of Metabolism and Physiology. Cell Metab 25, 118-127
27818261   Curated Info

3

Sacco F, et al. (2016) Glucose-regulated and drug-perturbed phosphoproteome reveals molecular mechanisms controlling insulin secretion. Nat Commun 7, 13250
27841257   Curated Info

4

Li J, et al. (2016) Long-term potentiation modulates synaptic phosphorylation networks and reshapes the structure of the postsynaptic interactome. Sci Signal 9, rs8
27507650   Curated Info

5

Nagai T, et al. (2016) Phosphoproteomics of the Dopamine Pathway Enables Discovery of Rap1 Activation as a Reward Signal In Vivo. Neuron 89, 550-65
26804993   Curated Info

6

Parker BL, et al. (2015) Targeted phosphoproteomics of insulin signaling using data-independent acquisition mass spectrometry. Sci Signal 8, rs6
26060331   Curated Info

7

Caohuy H, et al. (2014) Activation of 3-Phosphoinositide-dependent Kinase 1 (PDK1) and Serum- and Glucocorticoid-induced Protein Kinase 1 (SGK1) by Short-chain Sphingolipid C4-ceramide Rescues the Trafficking Defect of ΔF508-Cystic Fibrosis Transmembrane Conductance Regulator (ΔF508-CFTR). J Biol Chem 289, 35953-68
25384981   Curated Info

8

Reinartz M, Raupach A, Kaisers W, Gödecke A (2014) AKT1 and AKT2 induce distinct phosphorylation patterns in HL-1 cardiac myocytes. J Proteome Res 13, 4232-45
25162660   Curated Info

9

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

10

Wilson-Grady JT, Haas W, Gygi SP (2013) Quantitative comparison of the fasted and re-fed mouse liver phosphoproteomes using lower pH reductive dimethylation. Methods 61, 277-86
23567750   Curated Info

11

Humphrey SJ, et al. (2013) Dynamic Adipocyte Phosphoproteome Reveals that Akt Directly Regulates mTORC2. Cell Metab 17, 1009-20
23684622   Curated Info

12

Lundby A, et al. (2013) In vivo phosphoproteomics analysis reveals the cardiac targets of β-adrenergic receptor signaling. Sci Signal 6, rs11
23737553   Curated Info

13

Wu X, et al. (2012) Investigation of receptor interacting protein (RIP3)-dependent protein phosphorylation by quantitative phosphoproteomics. Mol Cell Proteomics 11, 1640-51
22942356   Curated Info

14

Grimsrud PA, et al. (2012) A quantitative map of the liver mitochondrial phosphoproteome reveals posttranslational control of ketogenesis. Cell Metab 16, 672-83
23140645   Curated Info

15

Biarc J, Chalkley RJ, Burlingame AL, Bradshaw RA (2012) The induction of serine/threonine protein phosphorylations by a PDGFR/TrkA chimera in stably transfected PC12 cells. Mol Cell Proteomics 11, 15-30
22027198   Curated Info

16

Chandran S, et al. (2011) Neural Precursor Cell-expressed Developmentally Down-regulated Protein 4-2 (Nedd4-2) Regulation by 14-3-3 Protein Binding at Canonical Serum and Glucocorticoid Kinase 1 (SGK1) Phosphorylation Sites. J Biol Chem 286, 37830-40
21900244   Curated Info

17

Hsu PP, et al. (2011) The mTOR-regulated phosphoproteome reveals a mechanism of mTORC1-mediated inhibition of growth factor signaling. Science 332, 1317-22
21659604   Curated Info

18

Navarro MN, et al. (2011) Phosphoproteomic analysis reveals an intrinsic pathway for the regulation of histone deacetylase 7 that controls the function of cytotoxic T lymphocytes. Nat Immunol 12, 352-61
21399638   Curated Info

19

Manes NP, et al. (2011) Discovery of mouse spleen signaling responses to anthrax using label-free quantitative phosphoproteomics via mass spectrometry. Mol Cell Proteomics 10, M110.000927
21189417   Curated Info

20

Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89
21183079   Curated Info

21

Guo A (2010) CST Curation Set: 9813; Year: 2010; Biosample/Treatment: cell line, mouse lung fibroblasts/untreated; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST]
Curated Info

22

Rinschen MM, et al. (2010) Quantitative phosphoproteomic analysis reveals vasopressin V2-receptor-dependent signaling pathways in renal collecting duct cells. Proc Natl Acad Sci U S A 107, 3882-7
20139300   Curated Info

23

Tweedie-Cullen RY, Reck JM, Mansuy IM (2009) Comprehensive mapping of post-translational modifications on synaptic, nuclear, and histone proteins in the adult mouse brain. J Proteome Res 8, 4966-82
19737024   Curated Info

24

Zanivan S, et al. (2008) Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry. J Proteome Res 7, 5314-26
19367708   Curated Info

25

Pan C, Gnad F, Olsen JV, Mann M (2008) Quantitative phosphoproteome analysis of a mouse liver cell line reveals specificity of phosphatase inhibitors. Proteomics 8, 4534-46
18846507   Curated Info

26

Liang X, Peters KW, Butterworth MB, Frizzell RA (2006) 14-3-3 isoforms are induced by aldosterone and participate in its regulation of epithelial sodium channels. J Biol Chem 281, 16323-32
16613846   Curated Info

27

Flores SY, et al. (2005) Aldosterone-induced serum and glucocorticoid-induced kinase 1 expression is accompanied by Nedd4-2 phosphorylation and increased Na+ transport in cortical collecting duct cells. J Am Soc Nephrol 16, 2279-87
15958725   Curated Info