Ser323
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Home > Phosphorylation Site Page: > Ser323  -  CK1E (human)

Site Information
RMGQLrGsAtRALPP   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 453416

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 6 , 7 ) , mass spectrometry ( 1 , 2 , 3 , 4 , 5 ) , mutation of modification site ( 6 , 7 ) , phosphopeptide mapping ( 6 )
Disease tissue studied:
neuroblastoma ( 6 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 7 ) , A498 (renal) ( 3 ) , HeLa S3 (cervical) ( 4 ) , HeLa_Meta (cervical) ( 2 ) , HeLa_Pro (cervical) ( 2 ) , HeLa_Telo (cervical) ( 2 ) , Jurkat (T lymphocyte) ( 1 , 5 ) , Neuro-2a (neuron) ( 6 )

Upstream Regulation
Putative in vivo kinases:
CK1E (human) ( 7 )
Putative upstream phosphatases:
PPP2CA (human) ( 7 )
Treatments:
ciclosporin ( 6 ) , DHPG ( 6 ) , MG132_withdrawal ( 2 ) , nocodazole ( 4 ) , thymidine ( 4 )

Downstream Regulation
Effects of modification on CK1E:
enzymatic activity, inhibited ( 6 , 7 )

References 

1

Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7
23749302   Curated Info

2

Dulla K, et al. (2010) Quantitative site-specific phosphorylation dynamics of human protein kinases during mitotic progression. Mol Cell Proteomics 9, 1167-81
20097925   Curated Info

3

Schreiber TB, et al. (2010) An integrated phosphoproteomics work flow reveals extensive network regulation in early lysophosphatidic acid signaling. Mol Cell Proteomics 9, 1047-62
20071362   Curated Info

4

Daub H, et al. (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle. Mol Cell 31, 438-48
18691976   Curated Info

5

Possemato A (2008) CST Curation Set: 3661; Year: 2008; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS:pSXXXpS
Curated Info

6

Liu F, Virshup DM, Nairn AC, Greengard P (2002) Mechanism of regulation of casein kinase I activity by group I metabotropic glutamate receptors. J Biol Chem 277, 45393-9
12223474   Curated Info

7

Gietzen KF, Virshup DM (1999) Identification of inhibitory autophosphorylation sites in casein kinase I epsilon. J Biol Chem 274, 32063-70
10542239   Curated Info