Ser22

Site Information
AEPAsPDsPKGssET SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 452618

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 1 , 3 , 4 , 5 , 6 , 8 ) , mutation of modification site ( 7 ) , western blotting ( 7 )
Disease tissue studied:	breast cancer ( 7 )
Relevant cell line - cell type - tissue:	'fat, brown' ( 6 ) , brain ( 4 , 6 ) , heart ( 6 ) , kidney ( 6 ) , liver ( 1 , 3 , 6 , 8 ) , lung ( 6 ) , MCF-7 (breast cell) ( 7 ) , MEF (fibroblast) [TSC2 (mouse), homozygous knockout] ( 5 ) , pancreas ( 6 ) , spleen ( 6 ) , testis ( 6 )

References
1	Robles MS, Humphrey SJ, Mann M (2017) Phosphorylation Is a Central Mechanism for Circadian Control of Metabolism and Physiology. Cell Metab 25, 118-127 27818261 Curated Info
2	Sacco F, et al. (2016) Glucose-regulated and drug-perturbed phosphoproteome reveals molecular mechanisms controlling insulin secretion. Nat Commun 7, 13250 27841257 Curated Info
3	Wilson-Grady JT, Haas W, Gygi SP (2013) Quantitative comparison of the fasted and re-fed mouse liver phosphoproteomes using lower pH reductive dimethylation. Methods 61, 277-86 23567750 Curated Info
4	Goswami T, et al. (2012) Comparative phosphoproteomic analysis of neonatal and adult murine brain. Proteomics 12, 2185-9 22807455 Curated Info
5	Yu Y, et al. (2011) Phosphoproteomic analysis identifies Grb10 as an mTORC1 substrate that negatively regulates insulin signaling. Science 332, 1322-6 21659605 Curated Info
6	Huttlin EL, et al. (2010) A tissue-specific atlas of mouse protein phosphorylation and expression. Cell 143, 1174-89 21183079 Curated Info
7	Vu EH, Kraus RJ, Mertz JE (2007) Phosphorylation-dependent sumoylation of estrogen-related receptor alpha1. Biochemistry 46, 9795-804 17676930 Curated Info
8	Villén J, Beausoleil SA, Gerber SA, Gygi SP (2007) Large-scale phosphorylation analysis of mouse liver. Proc Natl Acad Sci U S A 104, 1488-93 17242355 Curated Info