Ser532

Site Information
KIkQEVEsPtDKSGN SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 453087

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 ) , phosphopeptide mapping ( 10 )
Disease tissue studied:	breast cancer ( 3 ) , breast ductal carcinoma ( 3 ) , HER2 positive breast cancer ( 1 ) , luminal A breast cancer ( 1 ) , luminal B breast cancer ( 1 ) , breast cancer, triple negative ( 1 ) , leukemia ( 7 ) , acute myelogenous leukemia ( 7 )
Relevant cell line - cell type - tissue:	293N3S (epithelial) ( 4 ) , breast ( 1 , 3 ) , HeLa (cervical) ( 2 ) , Jurkat (T lymphocyte) ( 5 , 9 , 10 ) , K562 (erythroid) ( 6 , 8 ) , KG-1 (myeloid) ( 7 )

Upstream Regulation
Putative in vivo kinases:	ERK1 (human) ( 10 )
Kinases, in vitro:	ERK1 (human) ( 10 )
Treatments:	dasatinib ( 8 )

References
1	Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62 27251275 Curated Info
2	Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94 25159151 Curated Info
3	Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704 24719451 Curated Info
4	Tammsalu T, et al. (2014) Proteome-wide identification of SUMO2 modification sites. Sci Signal 7, rs2 24782567 Curated Info
5	Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7 23749302 Curated Info
6	Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71 23186163 Curated Info
7	Weber C, Schreiber TB, Daub H (2012) Dual phosphoproteomics and chemical proteomics analysis of erlotinib and gefitinib interference in acute myeloid leukemia cells. J Proteomics 75, 1343-56 22115753 Curated Info
8	Pan C, Olsen JV, Daub H, Mann M (2009) Global effects of kinase inhibitors on signaling networks revealed by quantitative phosphoproteomics. Mol Cell Proteomics 8, 2796-808 19651622 Curated Info
9	Possemato A (2009) CST Curation Set: 6369; Year: 2009; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pSP Curated Info
10	Vorbrueggen G, Lovrić J, Moelling K (1996) Functional analysis of phosphorylation at serine 532 of human c-Myb by MAP kinase. Biol Chem 377, 721-30 8960373 Curated Info