Ser1100
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Home > Phosphorylation Site Page: > Ser1100  -  IRS2 (human)

Site Information
PEAARVAsPTsGVKR   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 453065

In vivo Characterization
Methods used to characterize site in vivo:
immunoassay ( 5 ) , mass spectrometry ( 1 , 2 , 3 , 4 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 ) , phospho-antibody ( 5 ) , western blotting ( 5 )
Disease tissue studied:
breast cancer ( 5 , 7 , 8 ) , breast adenocarcinoma ( 5 ) , breast ductal carcinoma ( 7 ) , HER2 positive breast cancer ( 3 ) , luminal A breast cancer ( 3 ) , luminal B breast cancer ( 3 ) , breast cancer, surrounding tissue ( 3 ) , breast cancer, triple negative ( 3 , 5 , 7 ) , cervical cancer ( 21 ) , cervical adenocarcinoma ( 21 ) , colorectal cancer ( 5 ) , colorectal carcinoma ( 5 ) , leukemia ( 17 ) , acute myelogenous leukemia ( 17 ) , chronic lymphocytic leukemia ( 11 ) , lung cancer ( 5 , 10 , 16 ) , non-small cell lung cancer ( 5 , 16 ) , non-small cell lung adenocarcinoma ( 10 ) , mantle cell lymphoma ( 11 ) , neuroblastoma ( 15 ) , ovarian cancer ( 5 , 7 , 25 ) , prostate cancer ( 5 ) , melanoma skin cancer ( 1 , 5 , 22 , 25 )
Relevant cell line - cell type - tissue:
2048-mel (melanocyte) ( 5 , 22 ) , 293 (epithelial) [AT1 (human), transfection, AT1R stable transfected HEK293] ( 19 ) , 786-O (renal) [VHL (human), transfection] ( 4 ) , 786-O (renal) ( 4 ) , A498 (renal) ( 20 ) , blood ( 11 ) , breast ( 3 , 7 ) , BT-549 (breast cell) ( 5 ) , C32TG (melanocyte) ( 5 ) , colon ( 5 ) , COV413 (ovarian) ( 5 , 25 ) , dendritic cell ( 5 ) , DM331 (melanocyte) ( 25 ) , HCC366 (pulmonary) ( 16 ) , HCC78 (pulmonary) ( 16 ) , heart ( 5 ) , HeLa (cervical) ( 2 , 6 , 14 , 23 , 26 , 27 ) , HeLa S3 (cervical) ( 21 ) , HMLER ('stem, breast cancer') [CXCR4 (human), knockdown] ( 8 ) , HMLER ('stem, breast cancer') ( 8 ) , HOP62 (pulmonary) ( 16 ) , HUES-9 ('stem, embryonic') ( 18 ) , Jurkat (T lymphocyte) ( 12 ) , KG-1 (myeloid) ( 17 ) , liver ( 5 , 9 ) , LNCaP (prostate cell) ( 5 ) , lung ( 10 ) , MALME-3M (melanocyte) ( 5 ) , MCF-7 (breast cell) ( 5 ) , MDA-MB-231 (breast cell) ( 5 ) , MDA-MB-361 (breast cell) ( 5 ) , NB10 (neural crest) ( 15 ) , NCI-H1395 (pulmonary) ( 16 ) , NCI-H157 (pulmonary) ( 5 , 16 ) , NCI-H1666 (pulmonary) ( 16 ) , NCI-H2030 (pulmonary) ( 16 ) , NCI-H322 (pulmonary) ( 16 ) , NCI-H358 (pulmonary) ( 5 ) , NPC (neural crest) ( 15 ) , OV90 (ovarian) ( 5 ) , ovary ( 7 ) , OVCAR3 (ovarian) ( 5 ) , PC3 (prostate cell) ( 5 ) , platelet-blood ( 24 ) , SK-MEL24 (melanocyte) ( 5 ) , SK-MEL28 (melanocyte) ( 5 ) , skin ( 1 ) , SLM2 (melanocyte) ( 5 , 25 ) , SW480 (intestinal) ( 5 ) , SW620 (intestinal) ( 5 ) , T47D (breast cell) ( 5 ) , VBT2 ( 28 )

Upstream Regulation
Regulatory protein:
VHL (human) ( 4 )
Treatments:
EGF ( 2 ) , hypoxia ( 4 )

Downstream Regulation
Effects of modification on biological processes:
cell growth, inhibited ( 5 )

Disease / Diagnostics Relevance
Relevant diseases:
lung cancer ( 5 ) , ovarian cancer ( 25 ) , melanoma skin cancer ( 25 )

References 

1

Bassani-Sternberg M, et al. (2016) Direct identification of clinically relevant neoepitopes presented on native human melanoma tissue by mass spectrometry. Nat Commun 7, 13404
27869121   Curated Info

2

Huang H, et al. (2016) Simultaneous Enrichment of Cysteine-containing Peptides and Phosphopeptides Using a Cysteine-specific Phosphonate Adaptable Tag (CysPAT) in Combination with titanium dioxide (TiO2) Chromatography. Mol Cell Proteomics 15, 3282-3296
27281782   Curated Info

3

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

4

Malec V, Coulson JM, Urbé S, Clague MJ (2015) Combined Analyses of the VHL and Hypoxia Signaling Axes in an Isogenic Pairing of Renal Clear Cell Carcinoma Cells. J Proteome Res 14, 5263-72
26506913   Curated Info

5

Zarling AL, et al. (2014) MHC-restricted phosphopeptides from insulin receptor substrate-2 and CDC25b offer broad-based immunotherapeutic agents for cancer. Cancer Res 74, 6784-95
25297629   Curated Info

6

Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94
25159151   Curated Info

7

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

8

Yi T, et al. (2014) Quantitative phosphoproteomic analysis reveals system-wide signaling pathways downstream of SDF-1/CXCR4 in breast cancer stem cells. Proc Natl Acad Sci U S A 111, E2182-90
24782546   Curated Info

9

Bian Y, et al. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics 96, 253-62
24275569   Curated Info

10

Schweppe DK, Rigas JR, Gerber SA (2013) Quantitative phosphoproteomic profiling of human non-small cell lung cancer tumors. J Proteomics 91, 286-96
23911959   Curated Info

11

Cobbold M, et al. (2013) MHC class I-associated phosphopeptides are the targets of memory-like immunity in leukemia. Sci Transl Med 5, 203ra125
24048523   Curated Info

12

Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7
23749302   Curated Info

13

Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21
23312004   Curated Info

14

Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71
23186163   Curated Info

15

DeNardo BD, et al. (2013) Quantitative phosphoproteomic analysis identifies activation of the RET and IGF-1R/IR signaling pathways in neuroblastoma. PLoS One 8, e82513
24349301   Curated Info

16

Klammer M, et al. (2012) Phosphosignature predicts dasatinib response in non-small cell lung cancer. Mol Cell Proteomics 11, 651-68
22617229   Curated Info

17

Weber C, Schreiber TB, Daub H (2012) Dual phosphoproteomics and chemical proteomics analysis of erlotinib and gefitinib interference in acute myeloid leukemia cells. J Proteomics 75, 1343-56
22115753   Curated Info

18

Rigbolt KT, et al. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. Sci Signal 4, rs3
21406692   Curated Info

19

Christensen GL, et al. (2010) Quantitative phosphoproteomics dissection of seven-transmembrane receptor signaling using full and biased agonists. Mol Cell Proteomics 9, 1540-53
20363803   Curated Info

20

Schreiber TB, et al. (2010) An integrated phosphoproteomics work flow reveals extensive network regulation in early lysophosphatidic acid signaling. Mol Cell Proteomics 9, 1047-62
20071362   Curated Info

21

Olsen JV, et al. (2010) Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal 3, ra3
20068231   Curated Info

22

Depontieu FR, et al. (2009) Identification of tumor-associated, MHC class II-restricted phosphopeptides as targets for immunotherapy. Proc Natl Acad Sci U S A 106, 12073-8
19581576   Curated Info

23

Dephoure N, et al. (2008) A quantitative atlas of mitotic phosphorylation. Proc Natl Acad Sci U S A 105, 10762-7
18669648   Curated Info

24

Zahedi RP, et al. (2008) Phosphoproteome of resting human platelets. J Proteome Res 7, 526-34
18088087   Curated Info

25

Zarling AL, et al. (2006) Identification of class I MHC-associated phosphopeptides as targets for cancer immunotherapy. Proc Natl Acad Sci U S A 103, 14889-94
17001009   Curated Info

26

Beausoleil SA, et al. (2006) A probability-based approach for high-throughput protein phosphorylation analysis and site localization. Nat Biotechnol 24, 1285-92
16964243   Curated Info

27

Beausoleil SA, et al. (2004) Large-scale characterization of HeLa cell nuclear phosphoproteins. Proc Natl Acad Sci U S A 101, 12130-5
15302935   Curated Info

28

Zarling AL, et al. (2000) Phosphorylated peptides are naturally processed and presented by major histocompatibility complex class I molecules in vivo. J Exp Med 192, 1755-62
11120772   Curated Info