Ser513
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Home > Phosphorylation Site Page: > Ser513  -  dyskerin (human)

Site Information
AKEVELVsE______   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 452560

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 1 , 2 , 3 , 5 , 6 , 7 , 9 , 10 , 11 , 12 , 13 , 14 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 )
Disease tissue studied:
breast cancer ( 9 , 10 , 19 , 20 ) , breast ductal carcinoma ( 9 ) , HER2 positive breast cancer ( 3 ) , luminal A breast cancer ( 3 ) , luminal B breast cancer ( 3 ) , breast cancer, surrounding tissue ( 3 ) , breast cancer, triple negative ( 3 , 9 ) , cervical cancer ( 33 ) , cervical adenocarcinoma ( 33 ) , leukemia ( 23 , 43 ) , acute myelogenous leukemia ( 23 ) , acute erythroid leukemias, including erythroleukemia (M6a) and very rare pure erythroid leukemia (M6b) ( 18 ) , acute megakaryoblastic leukemia (M7) ( 18 ) , acute monoblastic leukemia (M5a) or acute monocytic leukemia (M5b) ( 18 ) , acute myeloblastic leukemia, with granulocytic maturation (M2) ( 18 ) , acute myeloblastic leukemia, without maturation (M1) ( 18 ) , chronic myelogenous leukemia ( 43 ) , lung cancer ( 7 , 12 , 20 , 26 ) , non-small cell lung cancer ( 20 ) , non-small cell lung adenocarcinoma ( 7 , 12 , 26 ) , B cell lymphoma ( 18 ) , non-Hodgkin's lymphoma ( 18 ) , neuroblastoma ( 17 ) , ovarian cancer ( 9 ) , pancreatic ductal adenocarcinoma ( 11 ) , multiple myeloma ( 18 ) , melanoma skin cancer ( 6 )
Relevant cell line - cell type - tissue:
'muscle, skeletal' ( 24 , 35 ) , 'pancreatic, ductal'-pancreas ( 11 ) , 293 (epithelial) [AT1 (human), transfection] ( 31 ) , 293 (epithelial) ( 38 ) , 293E (epithelial) ( 25 ) , A431 (epithelial) ( 52 ) , A549 (pulmonary) ( 13 ) , AML-193 (monocyte) ( 18 ) , breast ( 3 , 9 ) , BT-20 (breast cell) ( 20 ) , BT-549 (breast cell) ( 20 ) , Calu 6 (pulmonary) ( 20 ) , Chang liver (cervical) ( 46 ) , CMK (megakaryoblast) ( 18 ) , CTS (myeloid) ( 18 ) , DG75 (B lymphocyte) ( 30 ) , DOHH2 ('B lymphocyte, precursor') ( 18 ) , endothelial-aorta ( 21 ) , Flp-In T-Rex-293 (epithelial) [PRKD1 (human), genetic knockin] ( 22 ) , Flp-In T-Rex-293 (epithelial) ( 22 ) , H2009 (pulmonary) ( 20 ) , H2077 (pulmonary) ( 20 ) , H2887 (pulmonary) ( 20 ) , H322M (pulmonary) ( 20 ) , HCC1359 (pulmonary) ( 20 ) , HCC1937 (breast cell) ( 20 ) , HCC2279 (pulmonary) ( 20 ) , HCC366 (pulmonary) ( 20 ) , HCC4006 (pulmonary) ( 20 ) , HCC78 (pulmonary) ( 20 ) , HCC827 (pulmonary) ( 20 ) , HCT116 (intestinal) ( 37 , 39 ) , HEK293T (epithelial) ( 5 ) , HEL (erythroid) ( 18 ) , HeLa (cervical) ( 2 , 16 , 29 , 39 , 41 , 44 , 45 , 47 , 48 , 50 , 51 ) , HeLa S3 (cervical) [PLK1 (human), knockdown, Tet-inducible PLK1 siRNA] ( 27 ) , HeLa S3 (cervical) ( 27 , 33 , 36 ) , HeLa_Meta (cervical) ( 32 ) , HeLa_Pro (cervical) ( 32 ) , HeLa_Telo (cervical) ( 32 ) , HMLER ('stem, breast cancer') [CXCR4 (human), knockdown] ( 10 ) , HMLER ('stem, breast cancer') ( 10 ) , HOP62 (pulmonary) ( 20 ) , HUES-9 ('stem, embryonic') ( 28 ) , Jurkat (T lymphocyte) ( 14 , 40 ) , K562 (erythroid) ( 16 , 34 , 42 , 43 ) , Kasumi-1 (myeloid) ( 18 ) , KG-1 (myeloid) ( 18 , 23 ) , LCLC-103H (pulmonary) ( 20 ) , LOU-NH91 (squamous) ( 20 ) , lung ( 12 ) , lymphoblastoid ( 49 ) , MCF-7 (breast cell) ( 20 ) , MDA-MB-231 (breast cell) ( 20 ) , MDA-MB-468 (breast cell) ( 20 ) , MV4-11 (macrophage) ( 18 ) , NB10 (neural crest) ( 17 ) , NCI-H1395 (pulmonary) ( 20 ) , NCI-H1568 (pulmonary) ( 20 ) , NCI-H157 (pulmonary) ( 20 ) , NCI-H1648 (pulmonary) ( 20 ) , NCI-H1666 (pulmonary) ( 20 ) , NCI-H2030 (pulmonary) ( 20 ) , NCI-H2172 (pulmonary) ( 20 ) , NCI-H322 (pulmonary) ( 20 ) , NCI-H460 (pulmonary) ( 20 , 39 ) , NCI-H520 (squamous) ( 20 ) , NCI-H647 (pulmonary) ( 20 ) , NPC (neural crest) ( 17 ) , OPM-2 (plasma cell) ( 18 ) , ovary ( 9 ) , P31/FUJ (erythroid) ( 18 ) , PC9 (pulmonary) ( 7 , 20 ) , PC9-IR (pulmonary) ( 7 ) , RL ('B lymphocyte, precursor') ( 18 ) , RPMI-8266 (plasma cell) ( 18 ) , SKBr3 (breast cell) ( 19 ) , SU-DHL-6 (B lymphocyte) ( 18 ) , U-1810 (pulmonary) [EFNB3 (human), knockdown] ( 26 ) , U-1810 (pulmonary) ( 26 ) , U266 (plasma cell) ( 18 ) , Vero E6-S ('epithelial, kidney') ( 1 ) , WM239A (melanocyte) ( 6 )

Upstream Regulation
Treatments:
EGF ( 2 ) , nocodazole ( 33 ) , ZK-Thiazolidinone ( 27 )

References 

1

Bouhaddou M, et al. (2020) The Global Phosphorylation Landscape of SARS-CoV-2 Infection. Cell
32645325   Curated Info

2

Huang H, et al. (2016) Simultaneous Enrichment of Cysteine-containing Peptides and Phosphopeptides Using a Cysteine-specific Phosphonate Adaptable Tag (CysPAT) in Combination with titanium dioxide (TiO2) Chromatography. Mol Cell Proteomics 15, 3282-3296
27281782   Curated Info

3

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

4

Boeing S, et al. (2016) Multiomic Analysis of the UV-Induced DNA Damage Response. Cell Rep 15, 1597-1610
27184836   Curated Info

5

Franchin C, et al. (2015) Quantitative analysis of a phosphoproteome readily altered by the protein kinase CK2 inhibitor quinalizarin in HEK-293T cells. Biochim Biophys Acta 1854, 609-23
25278378   Curated Info

6

Stuart SA, et al. (2015) A Phosphoproteomic Comparison of B-RAFV600E and MKK1/2 Inhibitors in Melanoma Cells. Mol Cell Proteomics 14, 1599-615
25850435   Curated Info

7

Tsai CF, et al. (2015) Large-scale determination of absolute phosphorylation stoichiometries in human cells by motif-targeting quantitative proteomics. Nat Commun 6, 6622
25814448   Curated Info

8

Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94
25159151   Curated Info

9

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

10

Yi T, et al. (2014) Quantitative phosphoproteomic analysis reveals system-wide signaling pathways downstream of SDF-1/CXCR4 in breast cancer stem cells. Proc Natl Acad Sci U S A 111, E2182-90
24782546   Curated Info

11

Britton D, et al. (2014) Quantification of pancreatic cancer proteome and phosphorylome: indicates molecular events likely contributing to cancer and activity of drug targets. PLoS One 9, e90948
24670416   Curated Info

12

Schweppe DK, Rigas JR, Gerber SA (2013) Quantitative phosphoproteomic profiling of human non-small cell lung cancer tumors. J Proteomics 91, 286-96
23911959   Curated Info

13

Kim JY, et al. (2013) Dissection of TBK1 signaling via phosphoproteomics in lung cancer cells. Proc Natl Acad Sci U S A 110, 12414-9
23836654   Curated Info

14

Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7
23749302   Curated Info

15

Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21
23312004   Curated Info

16

Zhou H, et al. (2013) Toward a comprehensive characterization of a human cancer cell phosphoproteome. J Proteome Res 12, 260-71
23186163   Curated Info

17

DeNardo BD, et al. (2013) Quantitative phosphoproteomic analysis identifies activation of the RET and IGF-1R/IR signaling pathways in neuroblastoma. PLoS One 8, e82513
24349301   Curated Info

18

Casado P, et al. (2013) Phosphoproteomics data classify hematological cancer cell lines according to tumor type and sensitivity to kinase inhibitors. Genome Biol 14, R37
23628362   Curated Info

19

Imami K, et al. (2012) Temporal profiling of lapatinib-suppressed phosphorylation signals in EGFR/HER2 pathways. Mol Cell Proteomics 11, 1741-57
22964224   Curated Info

20

Klammer M, et al. (2012) Phosphosignature predicts dasatinib response in non-small cell lung cancer. Mol Cell Proteomics 11, 651-68
22617229   Curated Info

21

Verano-Braga T, et al. (2012) Time-resolved quantitative phosphoproteomics: new insights into Angiotensin-(1-7) signaling networks in human endothelial cells. J Proteome Res 11, 3370-81
22497526   Curated Info

22

Franz-Wachtel M, et al. (2012) Global detection of protein kinase D-dependent phosphorylation events in nocodazole-treated human cells. Mol Cell Proteomics 11, 160-70
22496350   Curated Info

23

Weber C, Schreiber TB, Daub H (2012) Dual phosphoproteomics and chemical proteomics analysis of erlotinib and gefitinib interference in acute myeloid leukemia cells. J Proteomics 75, 1343-56
22115753   Curated Info

24

Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876
22673903   Curated Info

25

Hsu PP, et al. (2011) The mTOR-regulated phosphoproteome reveals a mechanism of mTORC1-mediated inhibition of growth factor signaling. Science 332, 1317-22
21659604   Curated Info

26

Ståhl S, et al. (2011) Phosphoproteomic profiling of NSCLC cells reveals that ephrin B3 regulates pro-survival signaling through Akt1-mediated phosphorylation of the EphA2 receptor. J Proteome Res 10, 2566-78
21413766   Curated Info

27

Santamaria A, et al. (2011) The Plk1-dependent phosphoproteome of the early mitotic spindle. Mol Cell Proteomics 10, M110.004457
20860994   Curated Info

28

Rigbolt KT, et al. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. Sci Signal 4, rs3
21406692   Curated Info

29

Kettenbach AN, et al. (2011) Quantitative phosphoproteomics identifies substrates and functional modules of aurora and polo-like kinase activities in mitotic cells. Sci Signal 4, rs5
21712546   Curated Info

30

Iliuk AB, et al. (2010) In-depth analyses of kinase-dependent tyrosine phosphoproteomes based on metal ion-functionalized soluble nanopolymers. Mol Cell Proteomics 9, 2162-72
20562096   Curated Info

31

Xiao K, et al. (2010) Global phosphorylation analysis of beta-arrestin-mediated signaling downstream of a seven transmembrane receptor (7TMR). Proc Natl Acad Sci U S A 107, 15299-304
20686112   Curated Info

32

Dulla K, et al. (2010) Quantitative site-specific phosphorylation dynamics of human protein kinases during mitotic progression. Mol Cell Proteomics 9, 1167-81
20097925   Curated Info

33

Olsen JV, et al. (2010) Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. Sci Signal 3, ra3
20068231   Curated Info

34

Pan C, Olsen JV, Daub H, Mann M (2009) Global effects of kinase inhibitors on signaling networks revealed by quantitative phosphoproteomics. Mol Cell Proteomics 8, 2796-808
19651622   Curated Info

35

Højlund K, et al. (2009) In vivo phosphoproteome of human skeletal muscle revealed by phosphopeptide enrichment and HPLC-ESI-MS/MS. J Proteome Res 8, 4954-65
19764811   Curated Info

36

Malik R, et al. (2009) Quantitative analysis of the human spindle phosphoproteome at distinct mitotic stages. J Proteome Res 8, 4553-63
19691289   Curated Info

37

Oppermann FS, et al. (2009) Large-scale proteomics analysis of the human kinome. Mol Cell Proteomics 8, 1751-64
19369195   Curated Info

38

Gauci S, et al. (2009) Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach. Anal Chem 81, 4493-501
19413330   Curated Info

39

Nagano K, et al. (2009) Phosphoproteomic analysis of distinct tumor cell lines in response to nocodazole treatment. Proteomics 9, 2861-74
19415658   Curated Info

40

Mayya V, et al. (2009) Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions. Sci Signal 2, ra46
19690332   Curated Info

41

Dephoure N, et al. (2008) A quantitative atlas of mitotic phosphorylation. Proc Natl Acad Sci U S A 105, 10762-7
18669648   Curated Info

42

Stokes M (2008) CST Curation Set: 4609; Year: 2008; Biosample/Treatment: cell line, K562/untreated; Disease: chronic myelogenous leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY])
Curated Info

43

Stokes M (2008) CST Curation Set: 4393; Year: 2008; Biosample/Treatment: cell line, K562/untreated; Disease: chronic myelogenous leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[STY])
Curated Info

44

Ruse CI, et al. (2008) Motif-specific sampling of phosphoproteomes. J Proteome Res 7, 2140-50
18452278   Curated Info

45

McNulty DE, Annan RS (2008) Hydrophilic interaction chromatography reduces the complexity of the phosphoproteome and improves global phosphopeptide isolation and detection. Mol Cell Proteomics 7, 971-80
18212344   Curated Info

46

Sui S, et al. (2008) Phosphoproteome analysis of the human Chang liver cells using SCX and a complementary mass spectrometric strategy. Proteomics 8, 2024-34
18491316   Curated Info

47

Olsen JV, et al. (2006) Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Cell 127, 635-48
17081983   Curated Info

48

Beausoleil SA, et al. (2006) A probability-based approach for high-throughput protein phosphorylation analysis and site localization. Nat Biotechnol 24, 1285-92
16964243   Curated Info

49

Dirksen EH, et al. (2006) Investigating the dynamic nature of the interactions between nuclear proteins and histones upon DNA damage using an immobilized peptide chemical proteomics approach. J Proteome Res 5, 2380-8
16944950   Curated Info

50

Nousiainen M, et al. (2006) Phosphoproteome analysis of the human mitotic spindle. Proc Natl Acad Sci U S A 103, 5391-6
16565220   Curated Info

51

Beausoleil SA, et al. (2004) Large-scale characterization of HeLa cell nuclear phosphoproteins. Proc Natl Acad Sci U S A 101, 12130-5
15302935   Curated Info

52

MS This site is one of 509 sites observed by D. Stover et al using MS/FTMS of peptides from lysates of A431 cells grown either in vitro or as xenografts in BALB/c nu/nu mice. These sites were previously unpublished until now (July 27 2006). 66 sites were previously published in: Stover DR, et al. Differential phosphoprofiles of EGF and EGFR kinase inhibitor-treated human tumor cells and mouse xenografts Clin Proteomics 2004 Mar 01; 1(1): 69-80.
Curated Info