Ser277

Site Information
sRDRRHRsRsKsPGH SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 15722100
Available spectra: 2 CST

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 1 , 2 , 3 , 4 , 6 , 7 , 8 )
Disease tissue studied:	gastric cancer ( 7 ) , gastric carcinoma ( 7 ) , lung cancer ( 1 , 2 , 3 , 4 ) , non-small cell lung cancer ( 1 , 3 , 4 ) , non-small cell lung adenocarcinoma ( 1 , 2 , 3 , 4 ) , non-small cell large cell lung carcinoma ( 1 , 3 ) , non-small cell squamous cell lung carcinoma ( 2 )
Relevant cell line - cell type - tissue:	Cal-12T (pulmonary) ( 4 ) , HCC15 (pulmonary) ( 2 ) , HCC44 (pulmonary) ( 4 ) , HCC78 (pulmonary) ( 3 ) , Jurkat (T lymphocyte) ( 6 , 8 ) , LOU-NH91 (squamous) ( 2 ) , lung ( 1 , 2 , 3 , 4 ) , MKN-45 (gastric) ( 7 ) , NCI-H1299 (pulmonary) ( 1 ) , NCI-H1437 (pulmonary) ( 4 ) , NCI-H1666 (pulmonary) ( 4 ) , NCI-H1703 (squamous) ( 2 ) , NCI-H1734 (pulmonary) ( 1 ) , NCI-H1781 (pulmonary) ( 3 ) , NCI-H1944 (pulmonary) ( 1 ) , NCI-H2073 (pulmonary) ( 2 ) , NCI-H2228 (pulmonary) ( 3 ) , NCI-H2342 (pulmonary) ( 2 ) , NCI-H2405 (pulmonary) ( 4 ) , NCI-H3122 (pulmonary) ( 3 ) , NCI-H358 (pulmonary) ( 1 ) , NCI-H460 (pulmonary) ( 1 ) , NCI-H661 (pulmonary) ( 3 )

References
1	Rikova K, Hall B (2013) CST Curation Set: 20737, 21164, 30161, 30162, 30163; Year: 2013; Biosample/Treatment: cell line, H1299, H1944, H358, H1734, H460; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY, p[ST], RXXp[ST], pSQ, p[ST]QG, LXRXXp[ST], p[ST]P Curated Info
2	Rikova K, Hall B (2013) CST Curation Set: 20739, 21166, 30167, 30168, 30169; Year: 2013; Biosample/Treatment: cell line, H2342, H2073, Lou-NH91, HCC15, H1703; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY, p[ST], RXXp[ST], pSQ, p[ST]QG, LXRXXp[ST], p[ST]P Curated Info
3	Rikova K, Hall B (2013) CST Curation Set: 20740, 21167, 30170, 30171, 30172; Year: 2013; Biosample/Treatment: cell line, H2228, H3122, HCC78, H661, H1781; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY, p[ST], RXXp[ST], pSQ, p[ST]QG, LXRXXp[ST], p[ST]P Curated Info
4	Rikova K, Hall B (2013) CST Curation Set: 20741, 21168, 30173, 30174, 30175; Year: 2013; Biosample/Treatment: cell line, H1666, CAL-12T, H2405, HCC44, H1437; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY, p[ST], RXXp[ST], pSQ, p[ST]QG, LXRXXp[ST], p[ST]P Curated Info
5	Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21 23312004 Curated Info
6	Zhou J (2011) CST Curation Set: 12026; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY(YXXM) Antibodies Used to Purify Peptides prior to LCMS: Phospho-(Tyr) p85 PI3K Binding Motif Antibody Cat#: 3821 Curated Info
7	Moritz A (2011) CST Curation Set: 12374; Year: 2011; Biosample/Treatment: cell line, MKN-45/untreated; Disease: gastric carcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
8	Guo A (2011) CST Curation Set: 12067; Year: 2011; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P Curated Info