Thr403

Site Information
GCRKVTGtLDANRSS SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 15720201

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 1 , 2 , 3 , 4 , 5 , 6 ) , mutation of modification site ( 1 ) , phospho-antibody ( 1 ) , western blotting ( 1 )
Disease tissue studied:	breast cancer ( 5 ) , HER2 positive breast cancer ( 2 ) , luminal A breast cancer ( 2 ) , luminal B breast cancer ( 2 ) , breast cancer, surrounding tissue ( 2 ) , breast cancer, triple negative ( 2 , 5 ) , esophageal cancer ( 1 ) , esophageal carcinoma ( 1 ) , lung cancer ( 6 ) , non-small cell lung adenocarcinoma ( 6 ) , melanoma skin cancer ( 3 ) , cancer, squamous cell carcinoma ( 1 )
Relevant cell line - cell type - tissue:	breast ( 2 , 5 ) , esophagus ( 1 ) , HEK293T (epithelial) ( 1 ) , HeLa (cervical) ( 4 ) , Kyse150 (esophageal) ( 1 ) , Kyse450 (squamous) ( 1 ) , Kyse510 (esophageal) ( 1 ) , lung ( 6 ) , WM239A (melanocyte) ( 3 )

Downstream Regulation
Effects of modification on fascin:	protein conformation ( 1 )
Effects of modification on biological processes:	carcinogenesis, inhibited ( 1 ) , cell growth, inhibited ( 1 ) , cell motility, inhibited ( 1 ) , cytoskeletal reorganization ( 1 )

References
1	Zhang ZD, et al. (2022) AKT serine/threonine kinase 2-mediated phosphorylation of fascin threonine 403 regulates esophageal cancer progression. Int J Biochem Cell Biol 145, 106188 35219877 Curated Info
2	Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62 27251275 Curated Info
3	Stuart SA, et al. (2015) A Phosphoproteomic Comparison of B-RAFV600E and MKK1/2 Inhibitors in Melanoma Cells. Mol Cell Proteomics 14, 1599-615 25850435 Curated Info
4	Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94 25159151 Curated Info
5	Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704 24719451 Curated Info
6	Schweppe DK, Rigas JR, Gerber SA (2013) Quantitative phosphoproteomic profiling of human non-small cell lung cancer tumors. J Proteomics 91, 286-96 23911959 Curated Info
7	Shiromizu T, et al. (2013) Identification of missing proteins in the neXtProt database and unregistered phosphopeptides in the PhosphoSitePlus database as part of the Chromosome-centric Human Proteome Project. J Proteome Res 12, 2414-21 23312004 Curated Info