Ser524
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Home > Phosphorylation Site Page: > Ser524  -  5-LO (human)

Site Information
GMRGRKSsGFPkSVK   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 452140

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry (in vitro) ( 4 ) , mutation of modification site ( 5 , 6 ) , phospho-antibody ( 1 , 2 , 3 , 6 ) , western blotting ( 1 , 2 , 3 )
Disease tissue studied:
lymphoma ( 2 ) , B cell lymphoma ( 2 ) , non-Hodgkin's lymphoma ( 2 ) , mantle cell lymphoma ( 2 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 3 ) , 3T3 (fibroblast) [5-LO (human)] ( 6 ) , 3T3 (fibroblast) [PKIA (mouse)] ( 6 ) , 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout] ( 5 ) , GRANTA-519 (B lymphocyte) ( 2 ) , HEK293T (epithelial) ( 1 ) , JEKO-1 (B lymphocyte) ( 2 ) , REC-1 (B lymphocyte) ( 2 )

Upstream Regulation
Putative in vivo kinases:
PKACA (mouse) ( 5 , 6 )
Kinases, in vitro:
PKACA (human) ( 4 ) , PKACA (mouse) ( 6 )
Treatments:
cAMP_analog ( 2 , 5 ) , colforsin ( 5 ) , H-89 ( 5 ) , IBMX ( 5 ) , PGE2 ( 2 )

Downstream Regulation
Effects of modification on 5-LO:
enzymatic activity, inhibited ( 5 , 6 ) , intracellular localization ( 1 , 3 , 5 )

Disease / Diagnostics Relevance
Relevant diseases:
chronic lymphocytic leukemia ( 2 ) , mantle cell lymphoma ( 2 )

References 

1

Ball AK, et al. (2017) Characterization and cellular localization of human 5-lipoxygenase and its protein isoforms 5-LOΔ13, 5-LOΔ4 and 5-LOp12. Biochim Biophys Acta 1862, 561-571
28257804   Curated Info

2

Mahshid Y, et al. (2015) Phosphorylation of serine 523 on 5-lipoxygenase in human B lymphocytes. Prostaglandins Leukot Essent Fatty Acids 100, 33-40
26210919   Curated Info

3

Allain EP, Boudreau LH, Flamand N, Surette ME (2015) The Intracellular Localisation and Phosphorylation Profile of the Human 5-Lipoxygenase Δ13 Isoform Differs from That of Its Full Length Counterpart. PLoS One 10, e0132607
26173130   Curated Info

4

Markoutsa S, et al. (2014) Analysis of 5-lipoxygenase phosphorylation on molecular level by MALDI-MS. FEBS J 281, 1931-47
24860872   Curated Info

5

Luo M, et al. (2005) Phosphorylation by protein kinase a inhibits nuclear import of 5-lipoxygenase. J Biol Chem 280, 40609-16
16230355   Curated Info

6

Luo M, et al. (2004) Protein kinase A inhibits leukotriene synthesis by phosphorylation of 5-lipoxygenase on serine 523. J Biol Chem 279, 41512-20
15280375   Curated Info

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