Lys211

Site Information
tSGIFEtkFQVDkVN SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 15388968

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 1 , 2 , 3 , 4 , 5 )
Disease tissue studied:	multiple myeloma ( 5 )
Relevant cell line - cell type - tissue:	AMO-1 (plasma cell) ( 5 ) , HEK293T (epithelial) ( 4 ) , HEp-2 (squamous) ( 1 ) , Jurkat (T lymphocyte) ( 1 ) , MV4-11 (macrophage) ( 4 ) , U2OS (bone cell) ( 2 )

Upstream Regulation
Treatments:	MG132 ( 4 )

References
1	Akimov V, et al. (2018) UbiSite approach for comprehensive mapping of lysine and N-terminal ubiquitination sites. Nat Struct Mol Biol 25, 631-640 29967540 Curated Info
2	Halim VA, et al. (2018) Doxorubicin-induced DNA Damage Causes Extensive Ubiquitination of Ribosomal Proteins Associated with a Decrease in Protein Translation. Mol Cell Proteomics 29438997 Curated Info
3	Kim W, et al. (2011) Systematic and quantitative assessment of the ubiquitin-modified proteome. Mol Cell 44, 325-40 21906983 Curated Info
4	Wagner SA, et al. (2011) A Proteome-wide, Quantitative Survey of In Vivo Ubiquitylation Sites Reveals Widespread Regulatory Roles. Mol Cell Proteomics 10, M111.013284 21890473 Curated Info
5	Guo A (2010) CST Curation Set: 8771; Year: 2009; Biosample/Treatment: cell line, AMO-1/Velcade; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG) Antibodies Used to Purify Peptides prior to LCMS: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990 Curated Info