Lys742

Site Information
RLSAEQAkQLVSRVV SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 15387117

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 2 , 3 , 4 , 5 , 6 , 7 )
Disease tissue studied:	lung cancer ( 2 ) , non-small cell lung adenocarcinoma ( 2 ) , multiple myeloma ( 7 )
Relevant cell line - cell type - tissue:	A549 (pulmonary) ( 2 ) , AMO-1 (plasma cell) ( 7 ) , HEK293T (epithelial) ( 6 ) , Jurkat (T lymphocyte) ( 3 , 4 ) , MV4-11 (macrophage) ( 6 )

Upstream Regulation
Treatments:	vorinostat ( 2 )

References
1	Boeing S, et al. (2016) Multiomic Analysis of the UV-Induced DNA Damage Response. Cell Rep 15, 1597-1610 27184836 Curated Info
2	Wu Q, et al. (2015) Suberoylanilide hydroxamic acid treatment reveals crosstalks among proteome, ubiquitylome and acetylome in non-small cell lung cancer A549 cell line. Sci Rep 5, 9520 25825284 Curated Info
3	Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7 23749302 Curated Info
4	Udeshi ND, et al. (2013) Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments. Mol Cell Proteomics 12, 825-31 23266961 Curated Info
5	Kim W, et al. (2011) Systematic and quantitative assessment of the ubiquitin-modified proteome. Mol Cell 44, 325-40 21906983 Curated Info
6	Wagner SA, et al. (2011) A Proteome-wide, Quantitative Survey of In Vivo Ubiquitylation Sites Reveals Widespread Regulatory Roles. Mol Cell Proteomics 10, M111.013284 21890473 Curated Info
7	Guo A (2010) CST Curation Set: 9913; Year: 2010; Biosample/Treatment: cell line, AMO-1/Velcade; Disease: -; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: Ub(K-e-GG) Antibodies Used to Purify Peptides prior to LCMS: Ubiquitin (D4A7A10) XP(R) Rabbit mAb Cat#: 3925, PTMScan(R) Ubiquitin Branch Motif (K-e-GG) Immunoaffinity Beads Cat#: 1990 Curated Info