Tyr331

Site Information
PySEyFEyFAPDFTL SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 15228119

In vivo Characterization
Methods used to characterize site in vivo:	immunoprecipitation ( 1 , 2 ) , mass spectrometry ( 3 ) , mutation of modification site ( 1 , 2 ) , phospho-antibody ( 1 ) , western blotting ( 1 , 2 )
Disease tissue studied:	breast cancer ( 1 ) , colorectal cancer ( 2 ) , colorectal carcinoma ( 2 ) , leukemia ( 3 ) , chronic myelogenous leukemia ( 3 )
Relevant cell line - cell type - tissue:	HCT116 (intestinal) ( 2 ) , HEK293T (epithelial) ( 1 , 2 ) , K562 (erythroid) ( 3 ) , MCF-7 (breast cell) ( 1 ) , MDA-MB-231 (breast cell) ( 1 )

Downstream Regulation
Effects of modification on HDAC3:	activity, induced ( 2 ) , enzymatic activity, induced ( 1 ) , intracellular localization ( 1 )
Effects of modification on biological processes:	carcinogenesis, induced ( 1 ) , cell growth, induced ( 2 )

References
1	Kwak SM, et al. (2019) EGFR-c-Src-Mediated HDAC3 Phosphorylation Exacerbates Invasion of Breast Cancer Cells. Cells 8 31430896 Curated Info
2	Seo J, et al. (2018) Tyrosine phosphorylation of HDAC3 by Src kinase mediates proliferation of HER2-positive breast cancer cells. J Cell Physiol 234 30317579 Curated Info
3	Moritz A (2010) CST Curation Set: 9049; Year: 2010; Biosample/Treatment: cell line, K562/untreated & calyculin_A; Disease: chronic myelogenous leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info