Lys35
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Home > Acetylation Site Page: > Lys35  -  APE1 (human)

Site Information
TAAkkNDkEAAGEGP   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 15207805

In vivo Characterization
Methods used to characterize site in vivo:
immunoassay ( 1 ) , mass spectrometry ( 1 , 2 , 3 , 4 ) , modification-specific antibody ( 1 , 5 ) , mutation of modification site ( 1 ) , western blotting ( 1 )
Disease tissue studied:
breast cancer ( 5 ) , colorectal cancer ( 3 , 4 ) , colorectal carcinoma ( 3 , 4 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Treatments:
MMS ( 1 )

Downstream Regulation
Effects of modification on APE1:
acetylation ( 1 ) , enzymatic activity, induced ( 1 ) , intracellular localization ( 1 ) , molecular association, regulation ( 1 ) , protein conformation ( 1 )
Effects of modification on biological processes:
cell growth, inhibited ( 1 ) , DNA repair, induced ( 1 )
Inhibit interaction with:
NPM1 (human) ( 1 ) , RNA ( 1 )

References 

1

Lirussi L, et al. (2012) Nucleolar accumulation of APE1 depends on charged lysine residues that undergo acetylation upon genotoxic stress and modulate its BER activity in cells. Mol Biol Cell 23, 4079-96
22918947   Curated Info

2

Fantini D, et al. (2010) Critical lysine residues within the overlooked N-terminal domain of human APE1 regulate its biological functions. Nucleic Acids Res 38, 8239-56
20699270   Curated Info

3

Moritz A (2010) CST Curation Set: 9796; Year: 2010; Biosample/Treatment: cell line, HCT 116/untreated; Disease: colorectal carcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: acK
Curated Info

4

Moritz A (2010) CST Curation Set: 9797; Year: 2010; Biosample/Treatment: cell line, HCT 116/untreated; Disease: colorectal carcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: acK
Curated Info

5

Sandomenico A, et al. Monoclonal antibodies against pools of mono- and polyacetylated peptides selectively recognize acetylated lysines within the context of the original antigen. MAbs 8, 1575-1589
27560983   Curated Info