Lys32
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Home > Acetylation Site Page: > Lys32  -  APE1 (human)

Site Information
kSkTAAkkNDkEAAG   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 15207803

In vivo Characterization
Methods used to characterize site in vivo:
immunoassay ( 3 ) , mass spectrometry ( 2 , 3 , 4 ) , modification-specific antibody ( 3 , 5 ) , mutation of modification site ( 1 , 3 , 4 ) , western blotting ( 3 )
Disease tissue studied:
breast cancer ( 5 ) , colorectal cancer ( 1 ) , colorectal carcinoma ( 1 )
Relevant cell line - cell type - tissue:
E.coli (bacterial) ( 4 ) , HCT116 (intestinal) ( 1 ) , HEK293T (epithelial) ( 1 ) , HeLa (cervical) ( 2 , 3 , 4 , 5 )

Upstream Regulation
Treatments:
MMS ( 3 )

Downstream Regulation
Effects of modification on APE1:
acetylation ( 3 ) , enzymatic activity, induced ( 3 , 4 ) , intracellular localization ( 3 , 4 ) , molecular association, regulation ( 1 , 3 , 4 ) , protein conformation ( 3 )
Effects of modification on biological processes:
cell growth, induced ( 1 ) , cell growth, inhibited ( 3 ) , DNA repair, induced ( 1 , 3 , 4 )
Induce interaction with:
XRCC1 (human) ( 1 )
Inhibit interaction with:
NPM1 (human) ( 3 , 4 ) , RNA ( 3 , 4 )

References 

1

Roychoudhury S, et al. (2017) Human Apurinic/Apyrimidinic Endonuclease (APE1) Is Acetylated at DNA Damage Sites in Chromatin, and Acetylation Modulates Its DNA Repair Activity. Mol Cell Biol 37
27994014   Curated Info

2

Weinert BT, et al. (2013) Lysine succinylation is a frequently occurring modification in prokaryotes and eukaryotes and extensively overlaps with acetylation. Cell Rep 4, 842-51
23954790   Curated Info

3

Lirussi L, et al. (2012) Nucleolar accumulation of APE1 depends on charged lysine residues that undergo acetylation upon genotoxic stress and modulate its BER activity in cells. Mol Biol Cell 23, 4079-96
22918947   Curated Info

4

Fantini D, et al. (2010) Critical lysine residues within the overlooked N-terminal domain of human APE1 regulate its biological functions. Nucleic Acids Res 38, 8239-56
20699270   Curated Info

5

Sandomenico A, et al. Monoclonal antibodies against pools of mono- and polyacetylated peptides selectively recognize acetylated lysines within the context of the original antigen. MAbs 8, 1575-1589
27560983   Curated Info