Ser250

Site Information
TENAFsPsRSGGRAs SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 15176300

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 1 , 2 )
Relevant cell line - cell type - tissue:	3T3 (fibroblast) ( 2 ) , MEF (fibroblast) [TSC2 (mouse), homozygous knockout] ( 1 )

Upstream Regulation
Treatments:	NAG-thiazoline ( 2 ) , okadaic_acid ( 2 ) , PUGNAc ( 2 )

References
1	Yu Y, et al. (2011) Phosphoproteomic analysis identifies Grb10 as an mTORC1 substrate that negatively regulates insulin signaling. Science 332, 1322-6 21659605 Curated Info
2	Wang Z, Gucek M, Hart GW (2008) Cross-talk between GlcNAcylation and phosphorylation: site-specific phosphorylation dynamics in response to globally elevated O-GlcNAc. Proc Natl Acad Sci U S A 105, 13793-8 18779572 Curated Info