Thr853
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Home > Phosphorylation Site Page: > Thr853  -  MYPT1 (human)

Site Information
PREKRRstGVsFWtQ   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 452075

In vivo Characterization
Methods used to characterize site in vivo:
electrophoretic mobility shift ( 2 ) , mass spectrometry ( 4 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 16 , 17 , 18 , 19 , 20 , 22 ) , mutation of modification site ( 7 ) , phospho-antibody ( 1 , 2 , 3 , 5 , 6 , 7 , 15 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 ) , western blotting ( 1 , 2 , 3 , 5 , 6 , 7 , 15 , 23 , 24 , 25 , 26 , 28 , 29 , 30 , 31 , 33 , 35 )
Disease tissue studied:
leukemia ( 1 ) , acute myelogenous leukemia ( 1 ) , liver cancer ( 23 ) , neuroblastoma ( 4 ) , fibrosarcoma of soft tissue ( 33 )
Relevant cell line - cell type - tissue:
'muscle, skeletal' ( 27 ) , 'muscle, smooth' ( 15 , 35 ) , 'muscle, smooth'-'heart, artery' ( 29 ) , 293 (epithelial) ( 7 ) , 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout] ( 33 ) , BEL7402 (hepatic) ( 23 ) , CASMC ( 2 ) , cerebral artery ( 32 ) , COS (fibroblast) ( 7 , 33 ) , HEK293T (epithelial) ( 23 ) , HeLa (cervical) ( 23 ) , Hep 3B2.1-7 (hepatic) ( 23 ) , HepG2 (hepatic) ( 13 , 19 , 20 , 23 , 30 ) , HPAEC (endothelial) ( 34 ) , HSF (fibroblast) ( 6 ) , HT1080 (fibroblast) ( 33 ) , HUVEC (endothelial) ( 5 ) , LAN-6 (neural crest) ( 4 ) , NCI-H1650 (pulmonary) ( 8 ) , NCI-H2228 (pulmonary) ( 9 , 10 , 16 , 17 ) , NCI-H3255 (pulmonary) ( 11 , 12 , 14 , 18 , 22 ) , platelet-blood ( 3 , 28 ) , pulmonary artery ( 24 ) , SH-SY5Y (neural crest) ( 4 ) , SMMC7721 (hepatocyte) ( 23 ) , SMS-KCN-A (neural crest) ( 4 ) , Swiss 3T3 (fibroblast) ( 33 ) , thoracic aorta ( 25 ) , THP1 (myeloid) ( 1 ) , UASMC ( 2 ) , uterine artery ( 31 )

Upstream Regulation
Regulatory protein:
CADM4 (human) ( 5 ) , DLC1 (human) ( 23 ) , PPP1R12B (human) ( 7 ) , ROCK2 (human) ( 6 )
Putative in vivo kinases:
ROCK1 (human) ( 1 , 2 )
Kinases, in vitro:
PKCT (human) ( 21 ) , ROCK1 (human) ( 21 )
Treatments:
1,25_dihydroxyvitamin_D ( 1 ) , 5-HT ( 32 ) , arachidonic_acid ( 3 ) , Ca(2+) ( 35 ) , cGMP_analog ( 24 , 27 ) , colforsin ( 25 , 27 ) , depolarization ( 25 , 27 ) , development ( 29 ) , EGCG ( 1 ) , endothelin ( 24 , 35 ) , GF109203X ( 25 , 35 ) , GSK429286A ( 2 ) , GTP-gammaS ( 35 ) , H1152 ( 1 , 2 , 28 ) , HA-1077 ( 7 ) , hypoxia ( 24 , 29 ) , isoproterenol ( 25 ) , KCl ( 25 , 26 , 35 ) , LPA ( 6 ) , LY294002 ( 25 ) , muscle contraction ( 25 ) , nicardipine ( 26 ) , okadaic_acid ( 30 ) , PD98059 ( 31 ) , PGE2 ( 6 ) , phenylephrine ( 15 , 26 , 27 , 31 , 35 ) , phorbol_ester ( 1 , 15 ) , prazosin ( 35 ) , RGDS ( 28 ) , ryanodine ( 26 ) , sepsis ( 3 ) , serum ( 2 , 33 ) , serum_starvation ( 30 ) , siRNA ( 2 ) , SNP ( 15 ) , TGF-beta ( 6 ) , thrombin ( 28 , 34 ) , Y27632 ( 3 , 7 , 23 , 24 , 25 , 28 , 30 , 33 , 34 , 35 )

Downstream Regulation
Effects of modification on MYPT1:
enzymatic activity, inhibited ( 1 ) , phosphorylation ( 1 )
Effects of modification on biological processes:
cell adhesion, induced ( 1 ) , cell differentiation, induced ( 1 ) , cell growth, inhibited ( 1 ) , signaling pathway regulation ( 2 )

References 

1

Tóth E, Erdődi F, Kiss A (2021) Myosin Phosphatase Is Implicated in the Control of THP-1 Monocyte to Macrophage Differentiation. Int J Mol Sci 22
33802280   Curated Info

2

Deng JT, et al. (2019) Rho-associated kinase and zipper-interacting protein kinase, but not myosin light chain kinase, are involved in the regulation of myosin phosphorylation in serum-stimulated human arterial smooth muscle cells. PLoS One 14, e0226406
31834925   Curated Info

3

Reddi BA, et al. (2015) Attenuated platelet aggregation in patients with septic shock is independent from the activity state of myosin light chain phosphorylation or a reduction in Rho kinase-dependent inhibition of myosin light chain phosphatase. Intensive Care Med Exp 3, 37
26215804   Curated Info

4

Palacios-Moreno J, et al. (2015) Neuroblastoma Tyrosine Kinase Signaling Networks Involve FYN and LYN in Endosomes and Lipid Rafts. PLoS Comput Biol 11, e1004130
25884760   Curated Info

5

Yamana S, et al. (2015) The Cell Adhesion Molecule Necl-4/CADM4 Serves as a Novel Regulator for Contact Inhibition of Cell Movement and Proliferation. PLoS One 10, e0124259
25893857   Curated Info

6

Gerarduzzi C, He Q, Antoniou J, Di Battista JA (2014) Prostaglandin E(2)-dependent blockade of actomyosin and stress fibre formation is mediated through S1379 phosphorylation of ROCK2. J Cell Biochem 115, 1516-27
24610576   Curated Info

7

Shichi D, Arimura T, Ishikawa T, Kimura A (2010) Heart-specific small subunit of myosin light chain phosphatase activates rho-associated kinase and regulates phosphorylation of myosin phosphatase target subunit 1. J Biol Chem 285, 33680-90
20801872   Curated Info

8

Possemato A (2009) CST Curation Set: 8051; Year: 2009; Biosample/Treatment: cell line, NCI-H1650/untreated; Disease: non-small cell lung cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST]
Curated Info

9

Possemato A (2009) CST Curation Set: 7391; Year: 2009; Biosample/Treatment: cell line, NCI-H2228/-; Disease: non-small cell lung cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST]
Curated Info

10

Possemato A (2009) CST Curation Set: 7392; Year: 2009; Biosample/Treatment: cell line, NCI-H2228/-; Disease: non-small cell lung cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST]
Curated Info

11

Possemato A (2009) CST Curation Set: 7394; Year: 2009; Biosample/Treatment: cell line, NCI-H3255/-; Disease: non-small cell lung cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST]
Curated Info

12

Possemato A (2009) CST Curation Set: 7393; Year: 2009; Biosample/Treatment: cell line, NCI-H3255/-; Disease: non-small cell lung cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST]
Curated Info

13

Possemato A (2009) CST Curation Set: 7390; Year: 2009; Biosample/Treatment: cell line, HepG2/-; Disease: hepatocellular carcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST]
Curated Info

14

Possemato A (2009) CST Curation Set: 7305; Year: 2009; Biosample/Treatment: cell line, NCI-H3255/-; Disease: non-small cell lung cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST]
Curated Info

15

Kitazawa T, et al. (2009) Nitric oxide-induced biphasic mechanism of vascular relaxation via dephosphorylation of CPI-17 and MYPT1. J Physiol 587, 3587-603
19470783   Curated Info

16

Possemato A (2009) CST Curation Set: 7303; Year: 2009; Biosample/Treatment: cell line, NCI-H2228/-; Disease: non-small cell lung cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST]
Curated Info

17

Possemato A (2009) CST Curation Set: 7302; Year: 2009; Biosample/Treatment: cell line, NCI-H2228/-; Disease: non-small cell lung cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST]
Curated Info

18

Possemato A (2009) CST Curation Set: 7304; Year: 2009; Biosample/Treatment: cell line, NCI-H3255/-; Disease: non-small cell lung cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST]
Curated Info

19

Possemato A (2009) CST Curation Set: 7300; Year: 2009; Biosample/Treatment: cell line, HepG2/-; Disease: hepatocellular carcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST]
Curated Info

20

Possemato A (2009) CST Curation Set: 7299; Year: 2009; Biosample/Treatment: cell line, HepG2/-; Disease: hepatocellular carcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RXXp[ST]
Curated Info

21

Belkina NV, et al. (2009) LOK is a major ERM kinase in resting lymphocytes and regulates cytoskeletal rearrangement through ERM phosphorylation. Proc Natl Acad Sci U S A 106, 4707-12
19255442   Curated Info

22

Moritz A (2008) CST Curation Set: 5781; Year: 2008; Biosample/Treatment: cell line, NCI-H3255/Iressa; Disease: non-small cell lung cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RRXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-PKA Substrate (RRXS/T) (100G7) Rabbit mAb Cat#: 9624, PTMScan(R) Phospho-PKA Substrate Motif (K/RK/RXS*/T*) Immunoaffinity Beads Cat#: 1984
Curated Info

23

Wong CC, et al. (2008) Deleted in liver cancer 1 (DLC1) negatively regulates Rho/ROCK/MLC pathway in hepatocellular carcinoma. PLoS One 3, e2779
18648664   Curated Info

24

Gao Y, et al. (2007) Role of Rho kinases in PKG-mediated relaxation of pulmonary arteries of fetal lambs exposed to chronic high altitude hypoxia. Am J Physiol Lung Cell Mol Physiol 292, L678-84
17085525   Curated Info

25

Azam MA, et al. (2007) Ca2+-independent, inhibitory effects of cyclic adenosine 5'-monophosphate on Ca2+ regulation of phosphoinositide 3-kinase C2alpha, Rho, and myosin phosphatase in vascular smooth muscle. J Pharmacol Exp Ther 320, 907-16
17110524   Curated Info

26

Dimopoulos GJ, et al. (2007) Ca2+-dependent rapid Ca2+ sensitization of contraction in arterial smooth muscle. Circ Res 100, 121-9
17158339   Curated Info

27

Porter M, et al. (2006) Convergence of Ca2+-desensitizing mechanisms activated by forskolin and phenylephrine pretreatment, but not 8-bromo-cGMP. Am J Physiol Cell Physiol 290, C1552-9
16421202   Curated Info

28

Pandey D, Goyal P, Bamburg JR, Siess W (2006) Regulation of LIM-kinase 1 and cofilin in thrombin-stimulated platelets. Blood 107, 575-83
16219803   Curated Info

29

Dakshinamurti S, Mellow L, Stephens NL (2005) Regulation of pulmonary arterial myosin phosphatase activity in neonatal circulatory transition and in hypoxic pulmonary hypertension: a role for CPI-17. Pediatr Pulmonol 40, 398-407
16130142   Curated Info

30

Lontay B, et al. (2005) Okadaic acid induces phosphorylation and translocation of myosin phosphatase target subunit 1 influencing myosin phosphorylation, stress fiber assembly and cell migration in HepG2 cells. Cell Signal 17, 1265-75
16038801   Curated Info

31

Xiao D, Longo LD, Zhang L (2005) Alpha1-adrenoceptor-mediated phosphorylation of MYPT-1 and CPI-17 in the uterine artery: role of ERK/PKC. Am J Physiol Heart Circ Physiol 288, H2828-35
15665049   Curated Info

32

Watanabe Y, Faraci FM, Heistad DD (2005) Activation of Rho-associated kinase during augmented contraction of the basilar artery to serotonin after subarachnoid hemorrhage. Am J Physiol Heart Circ Physiol 288, H2653-8
15665056   Curated Info

33

Ren XD, et al. (2004) Disruption of Rho signal transduction upon cell detachment. J Cell Sci 117, 3511-8
15226371   Curated Info

34

Birukova AA, et al. (2004) Role of Rho GTPases in thrombin-induced lung vascular endothelial cells barrier dysfunction. Microvasc Res 67, 64-77
14709404   Curated Info

35

Kitazawa T, Eto M, Woodsome TP, Khalequzzaman M (2003) Phosphorylation of the myosin phosphatase targeting subunit and CPI-17 during Ca2+ sensitization in rabbit smooth muscle. J Physiol 546, 879-89
12563012   Curated Info