Tyr720
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Home > Phosphorylation Site Page: > Tyr720  -  PDGFRA (human)

Site Information
ADEstRSyVILSFEN   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 452056

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 10 ) , mass spectrometry ( 2 , 3 , 4 , 7 ) , mutation of modification site ( 1 , 8 , 9 , 10 ) , phospho-antibody ( 1 , 5 , 6 , 9 , 10 ) , phosphopeptide mapping ( 10 ) , western blotting ( 1 , 5 , 9 )
Disease tissue studied:
brain cancer ( 2 ) , glioblastoma ( 2 ) , glioma ( 2 ) , leukemia ( 7 ) , acute lymphocytic leukemia ( 7 ) , lung cancer ( 3 ) , non-small cell squamous cell lung carcinoma ( 3 )
Relevant cell line - cell type - tissue:
293 (epithelial) ( 1 ) , 3T3 (fibroblast) [SHP-2 (mouse), homozygous knockout] ( 10 ) , brain ( 5 ) , CGTH-W-1 (thyroid cell) ( 6 ) , F cells (fibroblast) ( 9 ) , glial-brain ( 2 ) , NCI-H1703 (squamous) ( 3 , 4 ) , PAE (endothelial) ( 8 ) , SUP-T13 (T lymphocyte) ( 7 )

Upstream Regulation
Treatments:
PDGF ( 9 )

Downstream Regulation
Effects of modification on PDGFRA:
molecular association, regulation ( 8 , 9 , 10 )
Induce interaction with:
SHP-2 (human) ( 8 , 10 ) , SHP-2 (mouse) ( 9 )

References 

1

Trairatphisan P, et al. (2016) A Probabilistic Boolean Network Approach for the Analysis of Cancer-Specific Signalling: A Case Study of Deregulated PDGF Signalling in GIST. PLoS One 11, e0156223
27232499   Curated Info

2

Johnson H, White FM (2014) Quantitative analysis of signaling networks across differentially embedded tumors highlights interpatient heterogeneity in human glioblastoma. J Proteome Res 13, 4581-93
24927040   Curated Info

3

Moritz A, et al. (2010) Akt-RSK-S6 Kinase Signaling Networks Activated by Oncogenic Receptor Tyrosine Kinases. Sci Signal 3, ra64
20736484   Curated Info

4

Moritz A (2009) CST Curation Set: 5955; Year: 2009; Biosample/Treatment: cell line, NCI-H1703/Gleevec; Disease: non-small cell lung cancer; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

5

Jackson EL, et al. (2006) PDGFR alpha-positive B cells are neural stem cells in the adult SVZ that form glioma-like growths in response to increased PDGF signaling. Neuron 51, 187-99
16846854   Curated Info

6

Chen KT, et al. (2006) An aberrant autocrine activation of the platelet-derived growth factor alpha-receptor in follicular and papillary thyroid carcinoma cell lines. Cancer Lett 231, 192-205
16126335   Curated Info

7

Gu T (2005) CST Curation Set: 731; Year: 2005; Biosample/Treatment: cell line, SUP-T13/-; Disease: acute lymphocytic leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991
Curated Info

8

Ekman S, et al. (2002) SHP-2 is involved in heterodimer specific loss of phosphorylation of Tyr771 in the PDGF beta-receptor. Oncogene 21, 1870-5
11896619   Curated Info

9

Ikuno Y, Leong FL, Kazlauskas A (2002) PI3K and PLCgamma play a central role in experimental PVR. Invest Ophthalmol Vis Sci 43, 483-9
11818394   Curated Info

10

Bazenet CE, Gelderloos JA, Kazlauskas A (1996) Phosphorylation of tyrosine 720 in the platelet-derived growth factor alpha receptor is required for binding of Grb2 and SHP-2 but not for activation of Ras or cell proliferation. Mol Cell Biol 16, 6926-36
8943348   Curated Info