Ser486
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Home > Phosphorylation Site Page: > Ser486  -  PFKFB2 (rat)

Site Information
IRRPRNYsVGSRPLK   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 452046

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 2 , 3 , 4 ) , phospho-antibody ( 1 ) , western blotting ( 1 )
Disease tissue studied:
adrenal cancer ( 3 , 4 ) , pheochromocytoma ( 3 , 4 )
Relevant cell line - cell type - tissue:
myocyte-heart ( 1 ) , PC-12 (chromaffin) ( 3 , 4 ) , stomach ( 2 ) , testis ( 2 )

Upstream Regulation
Treatments:
Akt-I-1,2 ( 1 ) , amino_acids ( 1 )

References 

1

Novellasdemunt L, et al. (2013) Akt-dependent activation of the heart 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFKFB2) isoenzyme by amino acids. J Biol Chem 288, 10640-51
23457334   Curated Info

2

Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876
22673903   Curated Info

3

Zhou J (2009) CST Curation Set: 6823; Year: 2009; Biosample/Treatment: cell line, PC-12/forskolin; Disease: pheochromocytoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RRXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-PKA Substrate (RRXS/T) (100G7) Rabbit mAb Cat#: 9624, PTMScan(R) Phospho-PKA Substrate Motif (K/RK/RXS*/T*) Immunoaffinity Beads Cat#: 1984
Curated Info

4

Zhou J (2009) CST Curation Set: 6822; Year: 2009; Biosample/Treatment: cell line, PC-12/untreated; Disease: pheochromocytoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: RRXp[ST] Antibodies Used to Purify Peptides prior to LCMS: Phospho-PKA Substrate (RRXS/T) (100G7) Rabbit mAb Cat#: 9624, PTMScan(R) Phospho-PKA Substrate Motif (K/RK/RXS*/T*) Immunoaffinity Beads Cat#: 1984
Curated Info

5

Deprez J, et al. (2000) Partial purification and characterization of a wortmannin-sensitive and insulin-stimulated protein kinase that activates heart 6-phosphofructo-2-kinase. Biochem J 347 Pt 1, 305-12
10727432   Curated Info