Ser224
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Home > Phosphorylation Site Page: > Ser224  -  GR (rat)

Site Information
DLEFSAGsPSKDTNE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447967

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 5 , 7 ) , electrophoretic mobility shift ( 5 ) , mass spectrometry ( 2 , 3 ) , mutation of modification site ( 5 ) , phospho-antibody ( 4 ) , phosphoamino acid analysis ( 5 ) , phosphopeptide mapping ( 5 , 6 , 7 ) , western blotting ( 1 , 6 )
Disease tissue studied:
bone cancer ( 4 , 6 ) , liver cancer ( 4 ) , hepatocellular carcinoma ( 4 )
Relevant cell line - cell type - tissue:
'brain, hippocampus' ( 1 ) , hepatic ( 4 , 7 ) , HTC (hepatic) ( 5 ) , IEC-6 (epithelial) ( 2 ) , liver ( 3 ) , U2OS (bone cell) ( 4 , 6 )

Upstream Regulation
Kinases, in vitro:
CDK2 (human) ( 7 )
Treatments:
chronic stress ( 1 ) , deacylcortivasol ( 5 ) , desoxycortone ( 5 ) , dexamethasone ( 4 , 5 ) , fluoxetine ( 1 ) , serum ( 2 )

Downstream Regulation
Effects of modification on GR:
intracellular localization ( 1 )

References 

1

Lee MS, et al. (2016) Temporal variability of glucocorticoid receptor activity is functionally important for the therapeutic action of fluoxetine in the hippocampus. Mol Psychiatry 21, 252-60
25330740   Curated Info

2

Courcelles M, et al. (2013) Phosphoproteome dynamics reveal novel ERK1/2 MAP kinase substrates with broad spectrum of functions. Mol Syst Biol 9, 669
23712012   Curated Info

3

Demirkan G, et al. (2011) Phosphoproteomic Profiling of In Vivo Signaling in Liver by the Mammalian Target of Rapamycin Complex 1 (mTORC1). PLoS One 6, e21729
21738781   Curated Info

4

Blind RD, Garabedian MJ (2008) Differential recruitment of glucocorticoid receptor phospho-isoforms to glucocorticoid-induced genes. J Steroid Biochem Mol Biol 109, 150-7
18304804   Curated Info

5

Pocuca N, et al. (1998) Using yeast to study glucocorticoid receptor phosphorylation. J Steroid Biochem Mol Biol 66, 303-18
9749836   Curated Info

6

Rogatsky I, Waase CL, Garabedian MJ (1998) Phosphorylation and inhibition of rat glucocorticoid receptor transcriptional activation by glycogen synthase kinase-3 (GSK-3). Species-specific differences between human and rat glucocorticoid receptor signaling as revealed through GSK-3 phosphorylation. J Biol Chem 273, 14315-21
9603939   Curated Info

7

Krstic MD, Rogatsky I, Yamamoto KR, Garabedian MJ (1997) Mitogen-activated and cyclin-dependent protein kinases selectively and differentially modulate transcriptional enhancement by the glucocorticoid receptor. Mol Cell Biol 17, 3947-54
9199329   Curated Info

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