Tyr870

Site Information
LGAEEKEyHAEGGKV SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 448061

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 1 ) , mutation of modification site ( 2 ) , phospho-antibody ( 2 , 3 , 4 , 5 , 6 ) , western blotting ( 2 , 3 , 6 )
Disease tissue studied:	adrenal cancer ( 1 , 6 ) , pheochromocytoma ( 1 , 6 )
Relevant cell line - cell type - tissue:	GN4 (epithelial) ( 5 ) , hepatocyte ( 3 ) , hepatocyte-liver ( 4 ) , MEF (fibroblast) ( 2 ) , PC-12 (chromaffin) ( 1 , 6 ) , R22 ('muscle, smooth') ( 2 ) , SYF (fibroblast) ( 2 ) , vascular smooth muscle cell ('muscle, smooth') ( 2 )

Upstream Regulation
Putative in vivo kinases:	Src (rat) ( 2 )
Treatments:	AG1478 ( 4 , 6 ) , bromocriptine ( 6 ) , cell_adhesion ( 3 ) , ciglitazone ( 5 ) , EGF ( 3 , 5 , 6 ) , Fas ( 4 ) , H-89 ( 3 ) , NAC ( 5 ) , nafenopin ( 5 ) , PP2 ( 3 , 5 , 6 ) , PP3 ( 5 ) , SU6656 ( 2 , 4 ) , TGF-beta ( 2 ) , troglitazone ( 5 ) , wortmannin ( 6 ) , Y27632 ( 2 )

References
1	Stokes M (2008) CST Curation Set: 4009; Year: 2008; Biosample/Treatment: cell line, PC-12/EGF; Disease: pheochromocytoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
2	Samarakoon R, Higgins SP, Higgins CE, Higgins PJ (2008) TGF-beta1-induced plasminogen activator inhibitor-1 expression in vascular smooth muscle cells requires pp60(c-src)/EGFR(Y845) and Rho/ROCK signaling. J Mol Cell Cardiol 44, 527-38 18255094 Curated Info
3	Fassett J, Tobolt D, Hansen LK (2006) Type I collagen structure regulates cell morphology and EGF signaling in primary rat hepatocytes through cAMP-dependent protein kinase A. Mol Biol Cell 17, 345-56 16251347 Curated Info
4	Reinehr R, et al. (2005) Involvement of NADPH oxidase isoforms and Src family kinases in CD95-dependent hepatocyte apoptosis. J Biol Chem 280, 27179-94 15917250 Curated Info
5	Gardner OS, et al. (2003) Dependence of peroxisome proliferator-activated receptor ligand-induced mitogen-activated protein kinase signaling on epidermal growth factor receptor transactivation. J Biol Chem 278, 46261-9 12966092 Curated Info
6	Nair VD, Sealfon SC (2003) Agonist-specific transactivation of phosphoinositide 3-kinase signaling pathway mediated by the dopamine D2 receptor. J Biol Chem 278, 47053-61 12970364 Curated Info