Tyr1196

Site Information
PTAENAEyLRVAPPS SwissProt Entrez-Gene
Blast this site against: NCBI SwissProt PDB
Site Group ID: 448060

In vivo Characterization
Methods used to characterize site in vivo:	mass spectrometry ( 1 , 2 , 4 , 5 , 6 ) , phospho-antibody ( 3 , 7 , 8 , 9 , 10 ) , western blotting ( 3 , 8 , 10 )
Disease tissue studied:	adrenal cancer ( 4 , 5 , 6 ) , pheochromocytoma ( 4 , 5 , 6 )
Relevant cell line - cell type - tissue:	A7r5 ('muscle, smooth') ( 9 , 10 ) , hepatocyte ( 8 ) , hepatocyte-liver ( 3 , 7 ) , liver ( 2 , 8 ) , myocyte ( 10 ) , PC-12 (chromaffin) ( 4 , 5 , 6 ) , Rat2 (fibroblast) ( 1 )

Upstream Regulation
Putative in vivo kinases:	EGFR (rat) ( 3 )
Treatments:	AG1478 ( 7 ) , apocynin ( 3 ) , DPI ( 3 ) , EGF ( 3 , 8 , 9 , 10 ) , Fas ( 7 ) , ouabain ( 9 , 10 ) , partial_hepatectomy ( 8 ) , phorbol_ester ( 8 ) , SU6656 ( 7 )

References
1	Zheng Y, et al. (2013) Temporal regulation of EGF signalling networks by the scaffold protein Shc1. Nature 499, 166-71 23846654 Curated Info
2	Lundby A, et al. (2012) Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues. Nat Commun 3, 876 22673903 Curated Info
3	Flinder LI, et al. (2011) EGF-induced ERK-activation downstream of FAK requires rac1-NADPH oxidase. J Cell Physiol 226, 2267-78 21660950 Curated Info
4	Guo A (2008) CST Curation Set: 5202; Year: 2008; Biosample/Treatment: cell line, PC-12/NGF; Disease: pheochromocytoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
5	Stokes M (2008) CST Curation Set: 4008; Year: 2008; Biosample/Treatment: cell line, PC-12/untreated; Disease: pheochromocytoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
6	Stokes M (2008) CST Curation Set: 4009; Year: 2008; Biosample/Treatment: cell line, PC-12/EGF; Disease: pheochromocytoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY Antibodies Used to Purify Peptides prior to LCMS: Phospho-Tyrosine Mouse mAb (P-Tyr-100) Cat#: 9411, PTMScan(R) Phospho-Tyr Motif (Y*) Immunoaffinity Beads Cat#: 1991 Curated Info
7	Reinehr R, et al. (2005) Involvement of NADPH oxidase isoforms and Src family kinases in CD95-dependent hepatocyte apoptosis. J Biol Chem 280, 27179-94 15917250 Curated Info
8	Skarpen E, et al. (2005) Altered regulation of EGF receptor signaling following a partial hepatectomy. J Cell Physiol 202, 707-16 15389569 Curated Info
9	Haas M, Wang H, Tian J, Xie Z (2002) Src-mediated inter-receptor cross-talk between the Na+/K+-ATPase and the epidermal growth factor receptor relays the signal from ouabain to mitogen-activated protein kinases. J Biol Chem 277, 18694-702 11907028 Curated Info
10	Haas M, Askari A, Xie Z (2000) Involvement of Src and epidermal growth factor receptor in the signal-transducing function of Na+/K+-ATPase. J Biol Chem 275, 27832-7 10874030 Curated Info