Ser369
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Home > Phosphorylation Site Page: > Ser369  -  RARA (human)

Site Information
YVRKRRPsRPHMFPK   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 451381

In vivo Characterization
Methods used to characterize site in vivo:
[32P] ATP in vitro ( 6 ) , immunoprecipitation ( 1 , 2 , 4 , 5 , 6 ) , mutation of modification site ( 3 , 4 , 5 , 6 ) , phospho-antibody ( 5 , 6 ) , western blotting ( 1 , 3 , 5 , 6 )
Disease tissue studied:
breast cancer ( 5 , 6 ) , leukemia ( 1 ) , acute myelogenous leukemia ( 1 ) , lymphoma ( 3 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
P38A (human) ( 5 ) , PKACA (human) ( 3 )
Putative in vivo kinases:
MSK1 (human) ( 5 ) , PKACA (human) ( 4 , 6 )
Kinases, in vitro:
MSK1 (human) ( 5 ) , PKACA (human) ( 6 )
Treatments:
cAMP_analog ( 4 ) , H-89 ( 5 ) , NKH_477 ( 6 ) , retinoic_acid ( 4 , 5 , 6 ) , SB203580 ( 5 ) , siRNA ( 5 ) , urofollitropin ( 4 )

Downstream Regulation
Effects of modification on RARA:
acetylation ( 3 ) , activity, inhibited ( 4 ) , intracellular localization ( 4 ) , molecular association, regulation ( 1 , 3 , 4 , 5 , 6 ) , phosphorylation ( 5 , 6 ) , protein conformation ( 2 , 5 , 6 ) , protein degradation ( 3 )
Effects of modification on biological processes:
cell differentiation, induced ( 3 ) , chromatin organization, altered ( 3 ) , signaling pathway regulation ( 5 , 6 ) , transcription, induced ( 3 , 5 , 6 ) , transcription, inhibited ( 4 )
Induce interaction with:
CCNH (human) ( 6 ) , DNA ( 5 ) , GTF2H1 (human) ( 5 ) , RARA iso2 (human) ( 1 )
Inhibit interaction with:
RXRA (human) ( 4 ) , SMRT (human) ( 3 )

References 

1

Gianni M, et al. (2017) RARĪ±2 and PML-RAR similarities in the control of basal and retinoic acid induced myeloid maturation of acute myeloid leukemia cells. Oncotarget 8, 37041-37060
27419624   Curated Info

2

Chebaro Y, et al. (2013) Phosphorylation of the retinoic acid receptor alpha induces a mechanical allosteric regulation and changes in internal dynamics. PLoS Comput Biol 9, e1003012
23637584   Curated Info

3

Jiao B, et al. (2013) 8-CPT-cAMP/all-trans retinoic acid targets t(11;17) acute promyelocytic leukemia through enhanced cell differentiation and PLZF/RARĪ± degradation. Proc Natl Acad Sci U S A 110, 3495-500
23382200   Curated Info

4

Santos NC, Kim KH (2010) Activity of retinoic acid receptor-alpha is directly regulated at its protein kinase A sites in response to follicle-stimulating hormone signaling. Endocrinology 151, 2361-72
20215566   Curated Info

5

Bruck N, et al. (2009) A coordinated phosphorylation cascade initiated by p38MAPK/MSK1 directs RARalpha to target promoters. EMBO J 28, 34-47
19078967   Curated Info

6

Gaillard E, et al. (2006) Phosphorylation by PKA potentiates retinoic acid receptor alpha activity by means of increasing interaction with and phosphorylation by cyclin H/cdk7. Proc Natl Acad Sci U S A 103, 9548-53
16769902   Curated Info