Ser179
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Home > Phosphorylation Site Page: > Ser179  -  RAP1B (human)

Site Information
PGkARKKsscQLL__   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 451799

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 4 ) , electrophoretic mobility shift ( 4 ) , immunoprecipitation ( 1 , 2 , 4 ) , mass spectrometry ( 3 ) , mutation of modification site ( 1 , 2 , 3 ) , phospho-antibody ( 3 ) , western blotting ( 1 , 2 , 3 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Putative in vivo kinases:
PKACA (human) ( 3 , 4 )
Kinases, in vitro:
PKACA (human) ( 4 , 5 ) , PKG1 (human) ( 5 )
Treatments:
H-89 ( 1 ) , N6-benzoyl-cAMP ( 3 ) , NKH_477 ( 4 )

Downstream Regulation
Effects of modification on RAP1B:
activity, induced ( 3 ) , molecular association, regulation ( 2 ) , protein conformation ( 3 )
Inhibit interaction with:
RAP1GDS1 (human) ( 2 )

References 

1

Zhang X, et al. (2018) Cyclase-Associated Protein 1 (CAP1) is a prenyl-binding partner of Rap1 GTPase. J Biol Chem
29618512   Curated Info

2

Wilson JM, et al. (2016) Differences in the Phosphorylation-Dependent Regulation of Prenylation of Rap1A and Rap1B. J Mol Biol 428, 4929-4945
27760305   Curated Info

3

Edreira MM, et al. (2009) Phosphorylation-induced conformational changes in Rap1b: allosteric effects on switch domains and effector loop. J Biol Chem 284, 27480-6
19651783   Curated Info

4

Altschuler D, Lapetina EG (1993) Mutational analysis of the cAMP-dependent protein kinase-mediated phosphorylation site of Rap1b. J Biol Chem 268, 7527-31
8463283   Curated Info

5

Miura Y, et al. (1992) Phosphorylation of smg p21B/rap1B p21 by cyclic GMP-dependent protein kinase. FEBS Lett 297, 171-4
1551424   Curated Info