Ser226
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Home > Phosphorylation Site Page: > Ser226  -  GR (human)

Site Information
IDENCLLsPLAGEDD   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 451796

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 19 ) , mass spectrometry ( 7 , 9 , 10 , 16 , 17 ) , mutation of modification site ( 13 , 18 , 19 , 20 ) , phospho-antibody ( 2 , 4 , 5 , 6 , 8 , 11 , 12 , 13 , 14 , 15 , 19 ) , western blotting ( 2 , 4 , 5 , 6 , 8 , 11 , 12 , 13 , 14 , 15 )
Disease tissue studied:
lung cancer ( 10 , 14 ) , non-small cell lung cancer ( 10 ) , lymphoma ( 6 , 11 , 12 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
P38A (human) ( 8 ) , PPP5C (human) ( 8 )
Putative in vivo kinases:
ERK1 (human) ( 15 ) , JNK1 (human) ( 15 , 20 ) , P38A (human) ( 12 , 15 ) , P38B (human) ( 12 )
Kinases, in vitro:
CDK5 (human) ( 18 ) , JNK1 (human) ( 20 )
Putative upstream phosphatases:
PPP5C (human) ( 19 )
Treatments:
amanitin ( 13 ) , bisindolylmaleimide ( 15 ) , calyculin_A ( 19 ) , dexamethasone ( 2 , 5 , 13 , 14 , 15 , 19 ) , etoposide ( 5 ) , fluticasone ( 8 ) , formoterol ( 11 ) , geldanamycin ( 19 ) , GnRH ( 15 ) , H-89 ( 15 ) , IL-2 ( 11 , 12 ) , IL-4 ( 11 , 12 ) , iturelix ( 15 ) , Methylprednisolone ( 2 ) , MG132 ( 13 ) , PD98059 ( 15 ) , SB203580 ( 8 , 15 ) , SP600125 ( 11 , 14 , 15 ) , triamcinolone ( 2 ) , UV ( 14 )

Downstream Regulation
Effects of modification on GR:
activity, inhibited ( 18 ) , intracellular localization ( 5 , 6 , 20 ) , molecular association, regulation ( 13 )
Effects of modification on biological processes:
transcription, induced ( 13 ) , transcription, inhibited ( 18 , 20 )
Induce interaction with:
GRIP1 (human) ( 13 )

References 

1

Nicoll JX, Fry AC, Mosier EM (2021) Androgen and glucocorticoid receptor phosphorylation following resistance exercise and pre-workout supplementation. Steroids 172, 108859
33974920   Curated Info

2

Nakamura R, et al. (2020) Complex fibroblast response to glucocorticoids may underlie variability of clinical efficacy in the vocal folds. Sci Rep 10, 20458
33235235   Curated Info

3

Lea S, et al. (2020) P38 MAPK and glucocorticoid receptor crosstalk in bronchial epithelial cells. J Mol Med (Berl)
31974640   Curated Info

4

Nicoll JX, Fry AC, Mosier EM (2019) Sex-based differences in resting MAPK, androgen, and glucocorticoid receptor phosphorylation in human skeletal muscle. Steroids 141, 23-29
30414425   Curated Info

5

Qattan MY, et al. (2017) Differential regulation of cell death pathways by the microenvironment correlates with chemoresistance and survival in leukaemia. PLoS One 12, e0178606
28582465   Curated Info

6

Kobayashi Y, et al. (2016) Protein tyrosine phosphatase PTP-RR regulates corticosteroid sensitivity. Respir Res 17, 30
27013170   Curated Info

7

Sharma K, et al. (2014) Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling. Cell Rep 8, 1583-94
25159151   Curated Info

8

Bouazza B, et al. (2014) Basal p38 Mitogen-Activated Protein Kinase Regulates Unliganded Glucocorticoid Receptor Function in Airway Smooth Muscle Cells. Am J Respir Cell Mol Biol 50, 301-15
24024586   Curated Info

9

Mertins P, et al. (2013) Integrated proteomic analysis of post-translational modifications by serial enrichment. Nat Methods 10, 634-7
23749302   Curated Info

10

Klammer M, et al. (2012) Phosphosignature predicts dasatinib response in non-small cell lung cancer. Mol Cell Proteomics 11, 651-68
22617229   Curated Info

11

Kobayashi Y, et al. (2012) Increased corticosteroid sensitivity by a long acting β2 agonist formoterol via β2 adrenoceptor independent protein phosphatase 2A activation. Pulm Pharmacol Ther 25, 201-7
22401993   Curated Info

12

Mercado N, et al. (2012) Restoration of Corticosteroid Sensitivity by p38 Mitogen Activated Protein Kinase Inhibition in Peripheral Blood Mononuclear Cells from Severe Asthma. PLoS One 7, e41582
22911818   Curated Info

13

Avenant C, Kotitschke A, Hapgood JP (2010) Glucocorticoid receptor phosphorylation modulates transcription efficacy through GRIP-1 recruitment. Biochemistry 49, 972-85
20047289   Curated Info

14

Lynch JT, et al. (2010) The role of glucocorticoid receptor phosphorylation in Mcl-1 and NOXA gene expression. Mol Cancer 9, 38
20156337   Curated Info

15

Kotitschke A, et al. (2009) Genomic and nongenomic cross talk between the gonadotropin-releasing hormone receptor and glucocorticoid receptor signaling pathways. Mol Endocrinol 23, 1726-45
19812390   Curated Info

16

Dephoure N, et al. (2008) A quantitative atlas of mitotic phosphorylation. Proc Natl Acad Sci U S A 105, 10762-7
18669648   Curated Info

17

Possemato A (2007) CST Curation Set: 3480; Year: 2007; Biosample/Treatment: cell line, Jurkat/calyculin_A & pervanadate; Disease: T cell leukemia; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P Antibodies Used to Purify Peptides prior to LCMS: Millipore 05-368
Curated Info

18

Kino T, et al. (2007) Cyclin-dependent kinase 5 differentially regulates the transcriptional activity of the glucocorticoid receptor through phosphorylation: clinical implications for the nervous system response to glucocorticoids and stress. Mol Endocrinol 21, 1552-68
17440046   Curated Info

19

Wang Z, et al. (2007) Modulation of glucocorticoid receptor phosphorylation and transcriptional activity by a C-terminal-associated protein phosphatase. Mol Endocrinol 21, 625-34
17185395   Curated Info

20

Itoh M, et al. (2002) Nuclear export of glucocorticoid receptor is enhanced by c-Jun N-terminal kinase-mediated phosphorylation. Mol Endocrinol 16, 2382-92
12351702   Curated Info