Thr432
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Home > Phosphorylation Site Page: > Thr432  -  Chk2 (human)

Site Information
PPFSEHRtQVsLkDQ   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447553

In vivo Characterization
Methods used to characterize site in vivo:
mass spectrometry ( 1 , 3 ) , phospho-antibody ( 2 , 3 , 4 ) , phosphopeptide mapping ( 1 ) , western blotting ( 2 , 3 , 4 )
Disease tissue studied:
colorectal cancer ( 2 ) , colorectal carcinoma ( 2 )
Relevant cell line - cell type - tissue:
A253 ( 4 ) , CHO (fibroblast) ( 2 ) , E.coli (bacterial) ( 1 , 3 ) , HCT15 (intestinal) ( 2 )

Upstream Regulation
Kinases, in vitro:
Chk2 (human) ( 1 )
Phosphatases, in vitro:
PPPM1A (human) ( 2 )
Treatments:
methylselenocysteine ( 4 ) , SN-38 ( 4 )

References 

1

Gabant G, et al. (2008) Autophosphorylated residues involved in the regulation of human chk2 in vitro. J Mol Biol 380, 489-503
18538787   Curated Info

2

Oliva-Trastoy M, et al. (2007) The Wip1 phosphatase (PPM1D) antagonizes activation of the Chk2 tumour suppressor kinase. Oncogene 26, 1449-58
16936775   Curated Info

3

King JB, et al. (2007) Identification of protein phosphorylation sites within Ser/Thr-rich cluster domains using site-directed mutagenesis and hybrid linear quadrupole ion trap Fourier transform ion cyclotron resonance mass spectrometry. Rapid Commun Mass Spectrom 21, 3443-51
17918214   Curated Info

4

Yin MB, et al. (2004) Enhanced 7-ethyl-10-hydroxycamptothecin (SN-38) lethality by methylselenocysteine is associated with Chk2 phosphorylation at threonine-68 and down-regulation of Cdc6 expression. Mol Pharmacol 66, 153-60
15213307   Curated Info