Tyr816
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Home > Phosphorylation Site Page: > Tyr816  -  TrkB (rat)

Site Information
LAkAsPVyLDILG__   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 451754

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 5 , 6 ) , immunoprecipitation ( 4 ) , mutation of modification site ( 4 , 5 ) , peptide sequencing ( 5 ) , phospho-antibody ( 1 , 2 , 3 ) , phosphoamino acid analysis ( 5 , 6 ) , phosphopeptide mapping ( 5 , 6 ) , western blotting ( 1 , 2 , 3 , 4 )
Relevant cell line - cell type - tissue:
'neuron, hippocampal'-brain ( 2 ) , 293 (epithelial) ( 1 ) , 3T3 (fibroblast) ( 4 ) , COS (fibroblast) ( 5 ) , neuron-'brain, hippocampus' ( 3 ) , Rat2 (fibroblast) ( 6 ) , SF9 ( 1 )

Upstream Regulation
Regulatory protein:
NGFR (rat) ( 1 )
Putative in vivo kinases:
TrkB (rat) ( 4 , 6 )
Treatments:
BDNF ( 3 , 4 ) , cAMP_analog ( 3 ) , colforsin ( 3 ) , desipramine ( 2 ) , electrical_stimulation ( 2 ) , fluoxetine ( 2 ) , isoproterenol ( 3 ) , K252a ( 2 ) , neurotrophin-3 ( 3 , 6 ) , Rp-cAMPS ( 3 ) , sertraline ( 2 ) , SKF38393 ( 3 )

Downstream Regulation
Effects of modification on TrkB:
molecular association, regulation ( 4 )
Effects of modification on biological processes:
signaling pathway regulation ( 2 ) , translation, altered ( 4 )
Induce interaction with:
CBL (mouse) ( 4 ) , PLCG1 (mouse) ( 4 )

References 

1

Saadipour K, et al. (2017) The transmembrane domain of the p75 neurotrophin receptor stimulates phosphorylation of the TrkB tyrosine kinase receptor. J Biol Chem 292, 16594-16604
28821608   Curated Info

2

Furmaga H, Carreno FR, Frazer A (2012) Vagal nerve stimulation rapidly activates brain-derived neurotrophic factor receptor TrkB in rat brain. PLoS One 7, e34844
22563458   Curated Info

3

Ji Y, Pang PT, Feng L, Lu B (2005) Cyclic AMP controls BDNF-induced TrkB phosphorylation and dendritic spine formation in mature hippocampal neurons. Nat Neurosci 8, 164-72
15665879   Curated Info

4

McCarty JH, Feinstein SC (1999) The TrkB receptor tyrosine kinase regulates cellular proliferation via signal transduction pathways involving SHC, PLCgamma, and CBL. J Recept Signal Transduct Res 19, 953-74
10533983   Curated Info

5

Guiton M, et al. (1995) Naturally occurring tyrosine kinase inserts block high affinity binding of phospholipase C gamma and Shc to TrkC and neurotrophin-3 signaling. J Biol Chem 270, 20384-90
7657612   Curated Info

6

Middlemas DS, Meisenhelder J, Hunter T (1994) Identification of TrkB autophosphorylation sites and evidence that phospholipase C-gamma 1 is a substrate of the TrkB receptor. J Biol Chem 269, 5458-66
8106527   Curated Info