Ser28
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Home > Phosphorylation Site Page: > Ser28  -  Chk2 (human)

Site Information
PHGsVtQsQGSSsQs   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447548

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 3 ) , electrophoretic mobility shift ( 3 ) , immunoprecipitation ( 3 ) , mutation of modification site ( 2 ) , phospho-antibody ( 1 , 2 , 3 ) , western blotting ( 1 )
Relevant cell line - cell type - tissue:
HEK293T (epithelial) ( 2 , 3 ) , HeLa (cervical) ( 1 ) , reticulocyte ( 3 ) , U2OS (bone cell) [GR (human)] ( 2 )

Upstream Regulation
Putative in vivo kinases:
ATM (human) ( 3 )
Treatments:
adriamycin ( 1 , 2 ) , caffeine ( 3 ) , hydroxyurea ( 2 ) , ionizing_radiation ( 2 , 3 ) , UV ( 2 ) , wortmannin ( 3 )

Downstream Regulation
Effects of modification on Chk2:
enzymatic activity, induced ( 3 ) , phosphorylation ( 3 )

References 

1

Livingstone M, et al. (2005) Valosin-containing protein phosphorylation at Ser784 in response to DNA damage. Cancer Res 65, 7533-40
16140914   Curated Info

2

Tsvetkov L, Xu X, Li J, Stern DF (2003) Polo-like kinase 1 and Chk2 interact and co-localize to centrosomes and the midbody. J Biol Chem 278, 8468-75
12493754   Curated Info

3

Xu X, Tsvetkov LM, Stern DF (2002) Chk2 activation and phosphorylation-dependent oligomerization. Mol Cell Biol 22, 4419-32
12024051   Curated Info

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