Thr26
Javascript is not enabled on this browser. This site will not work properly without Javascript.
PhosphoSitePlus Homepage PhosphoSitePlus® v6.5.9.3
Powered by Cell Signaling Technology
Home > Phosphorylation Site Page: > Thr26  -  Chk2 (human)

Site Information
sQPHGsVtQsQGSSs   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447547

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 4 ) , electrophoretic mobility shift ( 4 ) , immunoprecipitation ( 4 ) , mass spectrometry ( 1 ) , mutation of modification site ( 3 ) , phospho-antibody ( 2 , 3 , 4 ) , western blotting ( 2 )
Relevant cell line - cell type - tissue:
E.coli (bacterial) ( 1 ) , HEK293T (epithelial) ( 3 , 4 ) , HeLa (cervical) ( 2 ) , reticulocyte ( 4 ) , U2OS (bone cell) [GR (human)] ( 3 )

Upstream Regulation
Putative in vivo kinases:
ATM (human) ( 4 )
Treatments:
adriamycin ( 2 , 3 ) , caffeine ( 4 ) , hydroxyurea ( 3 ) , ionizing_radiation ( 3 , 4 ) , UV ( 3 ) , wortmannin ( 4 )

Downstream Regulation
Effects of modification on Chk2:
enzymatic activity, induced ( 4 ) , phosphorylation ( 4 )

References 

1

King JB, et al. (2007) Identification of protein phosphorylation sites within Ser/Thr-rich cluster domains using site-directed mutagenesis and hybrid linear quadrupole ion trap Fourier transform ion cyclotron resonance mass spectrometry. Rapid Commun Mass Spectrom 21, 3443-51
17918214   Curated Info

2

Livingstone M, et al. (2005) Valosin-containing protein phosphorylation at Ser784 in response to DNA damage. Cancer Res 65, 7533-40
16140914   Curated Info

3

Tsvetkov L, Xu X, Li J, Stern DF (2003) Polo-like kinase 1 and Chk2 interact and co-localize to centrosomes and the midbody. J Biol Chem 278, 8468-75
12493754   Curated Info

4

Xu X, Tsvetkov LM, Stern DF (2002) Chk2 activation and phosphorylation-dependent oligomerization. Mol Cell Biol 22, 4419-32
12024051   Curated Info