Ser19
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Home > Phosphorylation Site Page: > Ser19  -  Chk2 (human)

Site Information
sHGsSACsQPHGsVt   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447546

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 1 ) , mass spectrometry ( 6 ) , mutation of modification site ( 7 ) , phospho-antibody ( 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 ) , western blotting ( 1 , 2 , 3 , 4 , 5 , 6 , 8 , 11 )
Disease tissue studied:
colorectal cancer ( 4 ) , colorectal carcinoma ( 4 ) , prostate cancer ( 8 )
Relevant cell line - cell type - tissue:
A253 ( 11 ) , AT52RM (lymphoblastoid) ( 7 ) , CHO (fibroblast) ( 4 ) , E.coli (bacterial) ( 3 , 6 ) , GM07078 (lymphoblastoid) ( 7 ) , HCT15 (intestinal) ( 4 , 7 ) , HEK293T (epithelial) ( 1 , 10 ) , IMR-90 (fibroblast) ( 2 ) , LBC-N ( 7 ) , LCL (lymphoblastoid) ( 5 ) , OVCAR3 (ovarian) ( 9 ) , PC3 (prostate cell) ( 8 ) , SF9 ( 3 )

Upstream Regulation
Regulatory protein:
SOCS1 (human) ( 2 )
Kinases, in vitro:
Chk2 (human) ( 3 )
Phosphatases, in vitro:
PPPM1A (human) ( 4 )
Treatments:
4-nitroquinoline_1-oxide ( 7 ) , adriamycin ( 1 ) , caffeine ( 9 ) , hydroxyurea ( 1 , 7 ) , ionizing_radiation ( 1 , 5 , 7 ) , methylselenocysteine ( 11 ) , mitomycin_C ( 1 ) , resveratrol ( 9 ) , silibinin ( 8 ) , silymarin ( 8 ) , SN-38 ( 11 ) , UV ( 1 ) , VRX0466617 ( 5 )

Downstream Regulation
Effects of modification on Chk2:
enzymatic activity, induced ( 7 ) , molecular association, regulation ( 7 )
Effects of modification on biological processes:
cell cycle regulation ( 7 )
Induce interaction with:
Chk2 (human) ( 7 )

References 

1

Freeman AK, Dapic V, Monteiro AN (2010) Negative regulation of CHK2 activity by protein phosphatase 2A is modulated by DNA damage. Cell Cycle 9, 736-47
20160490   Curated Info

2

Calabrese V, et al. (2009) SOCS1 links cytokine signaling to p53 and senescence. Mol Cell 36, 754-67
20005840   Curated Info

3

Gabant G, et al. (2008) Autophosphorylated residues involved in the regulation of human chk2 in vitro. J Mol Biol 380, 489-503
18538787   Curated Info

4

Oliva-Trastoy M, et al. (2007) The Wip1 phosphatase (PPM1D) antagonizes activation of the Chk2 tumour suppressor kinase. Oncogene 26, 1449-58
16936775   Curated Info

5

Carlessi L, et al. (2007) Biochemical and cellular characterization of VRX0466617, a novel and selective inhibitor for the checkpoint kinase Chk2. Mol Cancer Ther 6, 935-44
17363488   Curated Info

6

King JB, et al. (2007) Identification of protein phosphorylation sites within Ser/Thr-rich cluster domains using site-directed mutagenesis and hybrid linear quadrupole ion trap Fourier transform ion cyclotron resonance mass spectrometry. Rapid Commun Mass Spectrom 21, 3443-51
17918214   Curated Info

7

Buscemi G, et al. (2006) DNA damage-induced cell cycle regulation and function of novel Chk2 phosphoresidues. Mol Cell Biol 26, 7832-45
16940182   Curated Info

8

Deep G, et al. (2006) Silymarin and silibinin cause G1 and G2-M cell cycle arrest via distinct circuitries in human prostate cancer PC3 cells: a comparison of flavanone silibinin with flavanolignan mixture silymarin. Oncogene 25, 1053-69
16205633   Curated Info

9

Tyagi A, et al. (2005) Resveratrol causes Cdc2-tyr15 phosphorylation via ATM/ATR-Chk1/2-Cdc25C pathway as a central mechanism for S phase arrest in human ovarian carcinoma Ovcar-3 cells. Carcinogenesis 26, 1978-87
15975956   Curated Info

10

Wei JH, et al. (2005) TTK/hMps1 participates in the regulation of DNA damage checkpoint response by phosphorylating CHK2 on threonine 68. J Biol Chem 280, 7748-57
15618221   Curated Info

11

Yin MB, et al. (2004) Enhanced 7-ethyl-10-hydroxycamptothecin (SN-38) lethality by methylselenocysteine is associated with Chk2 phosphorylation at threonine-68 and down-regulation of Cdc6 expression. Mol Pharmacol 66, 153-60
15213307   Curated Info