Ser370
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Home > Phosphorylation Site Page: > Ser370  -  p47phox (human)

Site Information
PAVPPRPsADLILNR   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 451730

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 8 , 9 , 10 ) , immunoprecipitation ( 2 , 3 ) , mutation of modification site ( 1 , 2 , 3 , 7 , 8 , 9 ) , peptide sequencing ( 10 ) , phospho-antibody ( 3 ) , phosphoamino acid analysis ( 10 ) , phosphopeptide mapping ( 9 , 10 ) , western blotting ( 3 )
Disease tissue studied:
leukemia ( 7 ) , chronic myelogenous leukemia ( 7 )
Relevant cell line - cell type - tissue:
B lymphocyte-spleen ( 8 , 9 ) , COS7 (fibroblast) ( 3 ) , K562 (erythroid) ( 7 ) , microvessel endothelial ( 1 ) , neutrophil ( 9 , 10 ) , Rat2 (fibroblast) ( 2 ) , SVEC (endothelial) ( 3 )

Upstream Regulation
Kinases, in vitro:
IRAK4 (human) ( 4 ) , PKACA (human) ( 9 ) , PKCA (human) ( 6 , 9 ) , PKCA (rat) ( 8 ) , PKCB iso2 (human) ( 6 ) , PKCD (human) ( 6 )

Downstream Regulation
Effects of modification on p47phox:
activity, induced ( 6 ) , enzymatic activity, induced ( 8 ) , intracellular localization ( 8 ) , molecular association, regulation ( 6 ) , phosphorylation ( 3 , 6 )
Effects of modification on biological processes:
signaling pathway regulation ( 3 )
Induce interaction with:
CYBA (human) ( 6 )

References 

1

Meijles DN, Fan LM, Howlin BJ, Li JM (2014) Molecular Insights of p47phox Phosphorylation Dynamics in the Regulation of NADPH Oxidase Activation and Superoxide Production. J Biol Chem 289, 22759-70
24970888   Curated Info

2

Park MT, et al. (2014) Novel signaling axis for ROS generation during K-Ras-induced cellular transformation. Cell Death Differ 21, 1185-97
24632950   Curated Info

3

Teng L, Fan LM, Meijles D, Li JM (2012) Divergent Effects of p47phox Phosphorylation at S303-4 or S379 on Tumor Necrosis Factor-α Signaling via TRAF4 and MAPK in Endothelial Cells. Arterioscler Thromb Vasc Biol 32, 1488-96
22460559   Curated Info

4

Pacquelet S, et al. (2007) Cross-talk between IRAK-4 and the NADPH oxidase. Biochem J 403, 451-61
17217339   Curated Info

5

Massenet C, et al. (2005) Effects of p47phox C terminus phosphorylations on binding interactions with p40phox and p67phox. Structural and functional comparison of p40phox and p67phox SH3 domains. J Biol Chem 280, 13752-61
15657040   Curated Info

6

Fontayne A, Dang PM, Gougerot-Pocidalo MA, El-Benna J (2002) Phosphorylation of p47phox sites by PKC alpha, beta II, delta, and zeta: effect on binding to p22phox and on NADPH oxidase activation. Biochemistry 41, 7743-50
12056906   Curated Info

7

Ago T, Nunoi H, Ito T, Sumimoto H (1999) Mechanism for phosphorylation-induced activation of the phagocyte NADPH oxidase protein p47(phox). Triple replacement of serines 303, 304, and 328 with aspartates disrupts the SH3 domain-mediated intramolecular interaction in p47(phox), thereby activating the oxidase. J Biol Chem 274, 33644-53
10559253   Curated Info

8

Johnson JL, et al. (1998) Activation of p47(PHOX), a cytosolic subunit of the leukocyte NADPH oxidase. Phosphorylation of ser-359 or ser-370 precedes phosphorylation at other sites and is required for activity. J Biol Chem 273, 35147-52
9857051   Curated Info

9

El Benna J, Faust RP, Johnson JL, Babior BM (1996) Phosphorylation of the respiratory burst oxidase subunit p47phox as determined by two-dimensional phosphopeptide mapping. Phosphorylation by protein kinase C, protein kinase A, and a mitogen-activated protein kinase. J Biol Chem 271, 6374-8
8626435   Curated Info

10

el Benna J, Faust LP, Babior BM (1994) The phosphorylation of the respiratory burst oxidase component p47phox during neutrophil activation. Phosphorylation of sites recognized by protein kinase C and by proline-directed kinases. J Biol Chem 269, 23431-6
8089108   Curated Info