Ser348
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Home > Phosphorylation Site Page: > Ser348  -  p47phox (human)

Site Information
PGPQsPGsPLEEERQ   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 451728

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 15 , 16 ) , immunoprecipitation ( 3 , 6 ) , mass spectrometry ( 1 , 4 , 5 , 7 , 8 , 9 , 10 ) , mutation of modification site ( 2 , 3 , 6 , 14 , 15 ) , peptide sequencing ( 16 ) , phospho-antibody ( 6 ) , phosphoamino acid analysis ( 16 ) , phosphopeptide mapping ( 15 , 16 ) , western blotting ( 6 )
Disease tissue studied:
HER2 positive breast cancer ( 1 ) , luminal A breast cancer ( 1 ) , luminal B breast cancer ( 1 ) , breast cancer, triple negative ( 1 ) , leukemia ( 14 ) , chronic myelogenous leukemia ( 14 ) , lung cancer ( 5 ) , non-small cell lung adenocarcinoma ( 5 ) , ovarian cancer ( 4 )
Relevant cell line - cell type - tissue:
B lymphocyte-spleen ( 15 ) , breast ( 1 ) , COS7 (fibroblast) ( 6 ) , DG75 (B lymphocyte) ( 7 ) , GM00130 (B lymphocyte) ( 8 ) , K562 (erythroid) ( 14 ) , leukocyte-blood ( 9 ) , lung ( 5 ) , microvessel endothelial ( 2 ) , neutrophil ( 15 , 16 ) , ovary ( 4 ) , Rat2 (fibroblast) ( 3 ) , SVEC (endothelial) ( 6 ) , T lymphocyte-blood ( 10 )

Upstream Regulation
Putative in vivo kinases:
PKCD (human) ( 3 )
Kinases, in vitro:
CK2A1 (human) ( 13 ) , ERK1 (human) ( 15 ) , IRAK4 (human) ( 11 ) , P38A (human) ( 12 ) , PKCD (human) ( 3 )
Treatments:
fMLP ( 16 ) , phorbol_ester ( 16 )

Downstream Regulation
Effects of modification on p47phox:
enzymatic activity, induced ( 3 ) , intracellular localization ( 3 )
Effects of modification on biological processes:
carcinogenesis, induced ( 3 ) , cell growth, induced ( 3 )

References 

1

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

2

Meijles DN, Fan LM, Howlin BJ, Li JM (2014) Molecular Insights of p47phox Phosphorylation Dynamics in the Regulation of NADPH Oxidase Activation and Superoxide Production. J Biol Chem 289, 22759-70
24970888   Curated Info

3

Park MT, et al. (2014) Novel signaling axis for ROS generation during K-Ras-induced cellular transformation. Cell Death Differ 21, 1185-97
24632950   Curated Info

4

Mertins P, et al. (2014) Ischemia in tumors induces early and sustained phosphorylation changes in stress kinase pathways but does not affect global protein levels. Mol Cell Proteomics 13, 1690-704
24719451   Curated Info

5

Schweppe DK, Rigas JR, Gerber SA (2013) Quantitative phosphoproteomic profiling of human non-small cell lung cancer tumors. J Proteomics 91, 286-96
23911959   Curated Info

6

Teng L, Fan LM, Meijles D, Li JM (2012) Divergent Effects of p47phox Phosphorylation at S303-4 or S379 on Tumor Necrosis Factor-α Signaling via TRAF4 and MAPK in Endothelial Cells. Arterioscler Thromb Vasc Biol 32, 1488-96
22460559   Curated Info

7

Iliuk AB, et al. (2010) In-depth analyses of kinase-dependent tyrosine phosphoproteomes based on metal ion-functionalized soluble nanopolymers. Mol Cell Proteomics 9, 2162-72
20562096   Curated Info

8

Bennetzen MV, et al. (2010) Site-specific phosphorylation dynamics of the nuclear proteome during the DNA damage response. Mol Cell Proteomics 9, 1314-23
20164059   Curated Info

9

Raijmakers R, et al. (2010) Exploring the human leukocyte phosphoproteome using a microfluidic reversed-phase-TiO2-reversed-phase high-performance liquid chromatography phosphochip coupled to a quadrupole time-of-flight mass spectrometer. Anal Chem 82, 824-32
20058876   Curated Info

10

Carrascal M, et al. (2008) Phosphorylation analysis of primary human T lymphocytes using sequential IMAC and titanium oxide enrichment. J Proteome Res 7, 5167-76
19367720   Curated Info

11

Pacquelet S, et al. (2007) Cross-talk between IRAK-4 and the NADPH oxidase. Biochem J 403, 451-61
17217339   Curated Info

12

Manke IA, et al. (2005) MAPKAP kinase-2 is a cell cycle checkpoint kinase that regulates the G2/M transition and S phase progression in response to UV irradiation. Mol Cell 17, 37-48
15629715   Curated Info

13

Park HS, et al. (2001) Phosphorylation of the leucocyte NADPH oxidase subunit p47(phox) by casein kinase 2: conformation-dependent phosphorylation and modulation of oxidase activity. Biochem J 358, 783-90
11535139   Curated Info

14

Ago T, Nunoi H, Ito T, Sumimoto H (1999) Mechanism for phosphorylation-induced activation of the phagocyte NADPH oxidase protein p47(phox). Triple replacement of serines 303, 304, and 328 with aspartates disrupts the SH3 domain-mediated intramolecular interaction in p47(phox), thereby activating the oxidase. J Biol Chem 274, 33644-53
10559253   Curated Info

15

El Benna J, Faust RP, Johnson JL, Babior BM (1996) Phosphorylation of the respiratory burst oxidase subunit p47phox as determined by two-dimensional phosphopeptide mapping. Phosphorylation by protein kinase C, protein kinase A, and a mitogen-activated protein kinase. J Biol Chem 271, 6374-8
8626435   Curated Info

16

el Benna J, Faust LP, Babior BM (1994) The phosphorylation of the respiratory burst oxidase component p47phox during neutrophil activation. Phosphorylation of sites recognized by protein kinase C and by proline-directed kinases. J Biol Chem 269, 23431-6
8089108   Curated Info