Ser315
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Home > Phosphorylation Site Page: > Ser315  -  p47phox (human)

Site Information
AHsIHQRsRKRLsQD   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 451725

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 9 ) , immunoprecipitation ( 5 ) , mutation of modification site ( 2 , 5 , 6 , 8 , 9 ) , phospho-antibody ( 1 , 3 , 4 , 5 , 6 ) , phosphopeptide mapping ( 9 ) , western blotting ( 1 , 3 , 4 , 5 , 6 )
Disease tissue studied:
leukemia ( 8 ) , chronic myelogenous leukemia ( 8 )
Relevant cell line - cell type - tissue:
B lymphocyte-blood ( 6 ) , B lymphocyte-spleen ( 9 ) , COS7 (fibroblast) ( 5 ) , K562 (erythroid) ( 8 ) , microvessel endothelial ( 2 ) , neutrophil ( 9 ) , neutrophil-blood ( 1 , 3 , 4 ) , SVEC (endothelial) ( 5 )

Upstream Regulation
Kinases, in vitro:
PKCA (human) ( 7 , 9 ) , PKCB iso2 (human) ( 7 ) , PKCD (human) ( 7 )
Treatments:
CL097 ( 4 ) , fMLP ( 4 ) , juglone ( 4 ) , LPS ( 1 ) , pansorbin ( 6 ) , phorbol_ester ( 4 , 6 ) , TNF ( 4 ) , zymosan ( 3 )

Downstream Regulation
Effects of modification on p47phox:
molecular association, regulation ( 7 )
Effects of modification on biological processes:
signaling pathway regulation ( 1 )
Induce interaction with:
CYBA (human) ( 7 )

References 

1

Liu M, et al. (2019) The Prolyl Isomerase Pin1 Controls Lipopolysaccharide-Induced Priming of NADPH Oxidase in Human Neutrophils. Front Immunol 10, 2567
31736979   Curated Info

2

Meijles DN, Fan LM, Howlin BJ, Li JM (2014) Molecular Insights of p47phox Phosphorylation Dynamics in the Regulation of NADPH Oxidase Activation and Superoxide Production. J Biol Chem 289, 22759-70
24970888   Curated Info

3

Makni-Maalej K, et al. (2013) Zymosan induces NADPH oxidase activation in human neutrophils by inducing the phosphorylation of p47phox and the activation of Rac2: involvement of protein tyrosine kinases, PI3Kinase, PKC, ERK1/2 and p38MAPkinase. Biochem Pharmacol 85, 92-100
23085266   Curated Info

4

Makni-Maalej K, et al. (2012) The TLR7/8 Agonist CL097 Primes N-Formyl-Methionyl-Leucyl-Phenylalanine-Stimulated NADPH Oxidase Activation in Human Neutrophils: Critical Role of p47phox Phosphorylation and the Proline Isomerase Pin1. J Immunol 189, 4657-65
23002436   Curated Info

5

Teng L, Fan LM, Meijles D, Li JM (2012) Divergent Effects of p47phox Phosphorylation at S303-4 or S379 on Tumor Necrosis Factor-α Signaling via TRAF4 and MAPK in Endothelial Cells. Arterioscler Thromb Vasc Biol 32, 1488-96
22460559   Curated Info

6

Belambri SA, et al. (2012) Phosphorylation of p47phox is required for receptor-mediated NADPH oxidase/NOX2 activation in Epstein-Barr virus-transformed human B lymphocytes. Am J Blood Res 2, 187-93
23119229   Curated Info

7

Fontayne A, Dang PM, Gougerot-Pocidalo MA, El-Benna J (2002) Phosphorylation of p47phox sites by PKC alpha, beta II, delta, and zeta: effect on binding to p22phox and on NADPH oxidase activation. Biochemistry 41, 7743-50
12056906   Curated Info

8

Ago T, Nunoi H, Ito T, Sumimoto H (1999) Mechanism for phosphorylation-induced activation of the phagocyte NADPH oxidase protein p47(phox). Triple replacement of serines 303, 304, and 328 with aspartates disrupts the SH3 domain-mediated intramolecular interaction in p47(phox), thereby activating the oxidase. J Biol Chem 274, 33644-53
10559253   Curated Info

9

El Benna J, Faust RP, Johnson JL, Babior BM (1996) Phosphorylation of the respiratory burst oxidase subunit p47phox as determined by two-dimensional phosphopeptide mapping. Phosphorylation by protein kinase C, protein kinase A, and a mitogen-activated protein kinase. J Biol Chem 271, 6374-8
8626435   Curated Info