Thr383
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Home > Phosphorylation Site Page: > Thr383  -  Chk2 (human)

Site Information
GEtsLMRtLCGtPty   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 447545
Available spectra:  1 CST

In vivo Characterization
Methods used to characterize site in vivo:
[32P] bio-synthetic labeling ( 9 , 13 , 14 ) , electrophoretic mobility shift ( 9 , 12 ) , immunoprecipitation ( 10 ) , mass spectrometry ( 2 , 3 , 4 , 5 , 6 , 7 , 8 , 10 ) , mutation of modification site ( 6 , 9 , 10 , 13 , 14 ) , phospho-antibody ( 6 , 9 , 12 , 13 , 14 ) , phosphopeptide mapping ( 9 , 13 ) , western blotting ( 6 , 9 , 10 , 12 )
Disease tissue studied:
ataxia-telangiectasia ( 12 ) , bone cancer ( 9 ) , brain cancer ( 12 ) , glioblastoma ( 12 ) , glioma ( 12 ) , lung cancer ( 2 , 3 , 4 , 5 ) , non-small cell lung cancer ( 4 , 5 ) , non-small cell lung adenocarcinoma ( 3 , 4 , 5 ) , non-small cell squamous cell lung carcinoma ( 3 ) , small-cell lung cancer ( 2 ) , Nijmegen Breakage Syndrome ( 12 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
Tax (retrovirus) ( 10 )
Putative in vivo kinases:
Chk2 (human) ( 9 , 13 , 14 )
Kinases, in vitro:
Chk2 (human) ( 9 , 13 ) , DNAPK (human) ( 12 )
Treatments:
camptothecin ( 12 ) , ionizing_radiation ( 6 , 10 , 12 , 13 , 14 )

Downstream Regulation
Effects of modification on Chk2:
enzymatic activity, induced ( 6 , 11 , 13 , 14 ) , intracellular localization ( 6 , 10 ) , protein conformation ( 11 ) , ubiquitination ( 6 )

References 

1

Petsalaki E, Zachos G (2014) Chk2 prevents mitotic exit when the majority of kinetochores are unattached. J Cell Biol 205, 339-56
24798733   Curated Info

2

Rikova K, Hall B (2013) CST Curation Set: 20735, 21162, 30155, 30156, 30157; Year: 2013; Biosample/Treatment: cell line, DMS53, H526, H69, H82, H446; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY, p[ST], RXXp[ST], pSQ, p[ST]QG, LXRXXp[ST], p[ST]P
Curated Info

3

Rikova K, Hall B (2013) CST Curation Set: 20739, 21166, 30167, 30168, 30169; Year: 2013; Biosample/Treatment: cell line, H2342, H2073, Lou-NH91, HCC15, H1703; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY, p[ST], RXXp[ST], pSQ, p[ST]QG, LXRXXp[ST], p[ST]P
Curated Info

4

Rikova K, Hall B (2013) CST Curation Set: 20742, 21169, 30176, 30177, 30178; Year: 2013; Biosample/Treatment: cell line, H838, DMS153, H2073, H209, H1437; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY, p[ST], RXXp[ST], pSQ, p[ST]QG, LXRXXp[ST], p[ST]P
Curated Info

5

Rikova K, Hall B (2013) CST Curation Set: 20741, 21168, 30173, 30174, 30175; Year: 2013; Biosample/Treatment: cell line, H1666, CAL-12T, H2405, HCC44, H1437; Disease: -; TMT: Y; Specificities of Antibodies Used to Purify Peptides prior to LCMS: pY, p[ST], RXXp[ST], pSQ, p[ST]QG, LXRXXp[ST], p[ST]P
Curated Info

6

Guo X, et al. (2010) Interdependent phosphorylation within the kinase domain T-loop Regulates CHK2 activity. J Biol Chem 285, 33348-57
20713355   Curated Info

7

Zhou J (2009) CST Curation Set: 7593; Year: 2009; Biosample/Treatment: cell line, HeLa/untreated; Disease: cervical adenocarcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
Curated Info

8

Zhou J (2009) CST Curation Set: 7594; Year: 2009; Biosample/Treatment: cell line, HeLa/untreated; Disease: cervical adenocarcinoma; SILAC: -; Specificities of Antibodies Used to Purify Peptides prior to LCMS: p[ST]P
Curated Info

9

Lovly CM, et al. (2008) Regulation of Chk2 ubiquitination and signaling through autophosphorylation of serine 379. Mol Cell Biol 28, 5874-85
18644861   Curated Info

10

Gupta SK, et al. (2007) Human T-cell leukemia virus type 1 Tax oncoprotein prevents DNA damage-induced chromatin egress of hyperphosphorylated Chk2. J Biol Chem 282, 29431-40
17698850   Curated Info

11

Oliver AW, et al. (2006) Trans-activation of the DNA-damage signalling protein kinase Chk2 by T-loop exchange. EMBO J 25, 3179-90
16794575   Curated Info

12

Li J, Stern DF (2005) Regulation of CHK2 by DNA-dependent protein kinase. J Biol Chem 280, 12041-50
15668230   Curated Info

13

Schwarz JK, Lovly CM, Piwnica-Worms H (2003) Regulation of the Chk2 protein kinase by oligomerization-mediated cis- and trans-phosphorylation. Mol Cancer Res 1, 598-609
12805407   Curated Info

14

Lee CH, Chung JH (2001) The hCds1 (Chk2)-FHA domain is essential for a chain of phosphorylation events on hCds1 that is induced by ionizing radiation. J Biol Chem 276, 30537-41
11390408   Curated Info