Ser194
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Home > Phosphorylation Site Page: > Ser194  -  IGFBP1 (human)

Site Information
LAKAQETsGEEIsKF   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 451660

In vivo Characterization
Methods used to characterize site in vivo:
2D analysis ( 15 ) , [32P] bio-synthetic labeling ( 21 , 22 ) , electrophoretic mobility shift ( 20 ) , immunoprecipitation ( 2 , 3 , 17 , 22 ) , mass spectrometry ( 1 , 2 , 3 , 6 , 7 , 8 , 9 , 12 , 13 , 14 , 16 , 17 , 18 , 19 , 20 ) , multiple reaction monitoring (MRM) ( 4 ) , mutation of modification site ( 15 ) , peptide sequencing ( 21 , 22 ) , phospho-antibody ( 1 , 3 , 4 , 5 , 6 , 8 , 9 , 10 , 11 , 15 ) , phosphoamino acid analysis ( 21 ) , western blotting ( 1 , 3 , 4 , 6 , 8 , 9 , 10 , 11 , 15 )
Disease tissue studied:
HER2 positive breast cancer ( 7 ) , luminal A breast cancer ( 7 ) , luminal B breast cancer ( 7 ) , breast cancer, triple negative ( 7 ) , liver cancer ( 1 , 3 , 8 , 9 , 10 , 11 , 20 ) , lung cancer ( 14 ) , non-small cell lung adenocarcinoma ( 14 ) , pancreatic ductal adenocarcinoma ( 13 ) , Fetal growth restriction ( 16 , 19 )
Relevant cell line - cell type - tissue:
'pancreatic, ductal'-pancreas ( 13 ) , amniotic fluid ( 16 , 17 , 18 , 19 ) , blood ( 11 ) , breast ( 7 ) , CHO (fibroblast) [EphB1 (human), transfection] ( 21 , 22 ) , CHO (fibroblast) ( 15 ) , endometrial stromal ( 2 , 4 ) , HepG2 (hepatic) ( 1 , 3 , 8 , 9 , 10 , 11 , 20 ) , liver ( 12 ) , lung ( 14 ) , stromal ( 6 )

Upstream Regulation
Regulatory protein:
DEPTOR (human) ( 10 , 11 ) , ERK1 (human) ( 10 ) , GCN2 (human) ( 10 ) , Raptor (human) ( 9 , 10 , 11 ) , RICTOR (human) ( 9 , 10 , 11 ) , TSC2 (human) ( 9 )
Putative in vivo kinases:
CK2A1 (human) ( 8 , 10 , 11 ) , PKACA (human) ( 2 ) , PKCA (human) ( 3 , 8 )
Kinases, in vitro:
CK2A1 (human) ( 8 )
Treatments:
8-Rp-cAMP ( 6 ) , amino_acid_starvation ( 20 ) , bisindolylmaleimide_II ( 3 ) , conditioned medium ( 1 ) , fetal growth restriction ( 11 ) , hypoxia ( 2 , 4 , 9 , 20 ) , leucine ( 10 ) , leucine_deprivation ( 3 , 6 , 8 , 10 ) , MPA ( 6 ) , phorbol_ester ( 3 ) , rapamycin ( 4 , 9 , 10 , 11 ) , siRNA ( 3 ) , TBB ( 3 , 10 ) , U0126 ( 10 )

Downstream Regulation
Effects of modification on IGFBP1:
activity, induced ( 20 ) , activity, inhibited ( 4 , 10 ) , molecular association, regulation ( 4 , 16 , 20 ) , phosphorylation ( 10 )
Effects of modification on biological processes:
signaling pathway regulation ( 4 )
Induce interaction with:
CK2B (human) ( 4 ) , IGF1 (human) ( 16 , 20 )

Disease / Diagnostics Relevance
Relevant diseases:
Fetal growth restriction ( 10 , 16 )

References 

1

Rosario FJ, et al. (2023) Placental Remote Control of Fetal Metabolism: Trophoblast mTOR Signaling Regulates Liver IGFBP-1 Phosphorylation and IGF-1 Bioavailability. Int J Mol Sci 24
37108437   Curated Info

2

Gupta MB, et al. (2022) Increased Colocalization and Interaction Between Decidual Protein Kinase A and Insulin-like Growth Factor-Binding Protein-1 in Intrauterine Growth Restriction. J Histochem Cytochem 70, 515-530
35801847   Curated Info

3

Chen AW, et al. (2021) IGFBP-1 hyperphosphorylation in response to nutrient deprivation is mediated by activation of protein kinase Cα (PKCα). Mol Cell Endocrinol 536, 111400
34314739   Curated Info

4

Abu Shehab M, et al. (2020) Inhibition of decidual IGF-1 signaling in response to hypoxia and leucine deprivation is mediated by mTOR and AAR pathways and increased IGFBP-1 phosphorylation. Mol Cell Endocrinol 512, 110865
32502935   Curated Info

5

Singal SS, et al. (2018) Increased Insulin-like Growth Factor Binding Protein-1 Phosphorylation in Decidualized Stromal Mesenchymal Cells in Human Intrauterine Growth Restriction Placentas. J Histochem Cytochem, 22155418772574
29718759   Curated Info

6

Shehab MA, et al. (2017) Exposure of decidualized HIESC to low oxygen tension and leucine deprivation results in increased IGFBP-1 phosphorylation and reduced IGF-I bioactivity. Mol Cell Endocrinol 452, 1-14
28435049   Curated Info

7

Mertins P, et al. (2016) Proteogenomics connects somatic mutations to signalling in breast cancer. Nature 534, 55-62
27251275   Curated Info

8

Malkani N, et al. (2016) Increased IGFBP-1 phosphorylation in response to leucine deprivation is mediated by CK2 and PKC. Mol Cell Endocrinol 425, 48-60
26733150   Curated Info

9

Damerill I, et al. (2016) Hypoxia Increases IGFBP-1 Phosphorylation Mediated by mTOR Inhibition. Mol Endocrinol 30, 201-16
26714229   Curated Info

10

Malkani N, Jansson T, Gupta MB (2015) IGFBP-1 hyperphosphorylation in response to leucine deprivation is mediated by the AAR pathway. Mol Cell Endocrinol 412, 182-95
25957086   Curated Info

11

Abu Shehab M, et al. (2014) Liver mTOR controls IGF-I bioavailability by regulation of protein kinase CK2 and IGFBP-1 phosphorylation in fetal growth restriction. Endocrinology 155, 1327-39
24437487   Curated Info

12

Bian Y, et al. (2014) An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. J Proteomics 96, 253-62
24275569   Curated Info

13

Britton D, et al. (2014) Quantification of pancreatic cancer proteome and phosphorylome: indicates molecular events likely contributing to cancer and activity of drug targets. PLoS One 9, e90948
24670416   Curated Info

14

Schweppe DK, Rigas JR, Gerber SA (2013) Quantitative phosphoproteomic profiling of human non-small cell lung cancer tumors. J Proteomics 91, 286-96
23911959   Curated Info

15

Abu Shehab M, et al. (2013) Phosphorylation of IGFBP-1 at Discrete Sites Elicits Variable Effects on IGF-I Receptor Autophosphorylation. Endocrinology 154, 1130-43
23354097   Curated Info

16

Abu Shehab M, et al. (2010) Site-specific IGFBP-1 hyper-phosphorylation in fetal growth restriction: clinical and functional relevance. J Proteome Res 9, 1873-81
20143870   Curated Info

17

Abu Shehab M, Inoue S, Han VK, Gupta MB (2009) Site specific phosphorylation of insulin-like growth factor binding protein-1 (IGFBP-1) for evaluating clinical relevancy in fetal growth restriction. J Proteome Res 8, 5325-35
19731965   Curated Info

18

Dolcini L, et al. (2009) Identification of the amniotic fluid insulin-like growth factor binding protein-1 phosphorylation sites and propensity to proteolysis of the isoforms. FEBS J 276, 6033-46
19765076   Curated Info

19

Nissum M, et al. (2009) Functional and complementary phosphorylation state attributes of human insulin-like growth factor-binding protein-1 (IGFBP-1) isoforms resolved by free flow electrophoresis. Mol Cell Proteomics 8, 1424-35
19193607   Curated Info

20

Seferovic MD, et al. (2009) Hypoxia and leucine deprivation induce human insulin-like growth factor binding protein-1 hyperphosphorylation and increase its biological activity. Endocrinology 150, 220-31
18772238   Curated Info

21

Jones JI, Busby WH, Wright G, Clemmons DR (1993) Human IGFBP-1 is phosphorylated on 3 serine residues: effects of site-directed mutagenesis of the major phosphoserine. Growth Regul 3, 37-40
7683525   Curated Info

22

Jones JI, et al. (1993) Identification of the sites of phosphorylation in insulin-like growth factor binding protein-1. Regulation of its affinity by phosphorylation of serine 101. J Biol Chem 268, 1125-31
7678248   Curated Info