Ser349
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Home > Phosphorylation Site Page: > Ser349  -  PRLR (human)

Site Information
YLDPDTDsGRGSCDs   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 451641

In vivo Characterization
Methods used to characterize site in vivo:
immunoprecipitation ( 2 , 3 ) , mutation of modification site ( 1 , 2 , 3 , 4 ) , phospho-antibody ( 2 , 3 ) , western blotting ( 1 , 2 , 3 )
Disease tissue studied:
breast cancer ( 1 , 3 ) , breast adenocarcinoma ( 3 ) , breast cancer, triple negative ( 3 )
Relevant cell line - cell type - tissue:

Upstream Regulation
Regulatory protein:
prolactin (human) ( 3 )
Putative in vivo kinases:
JAK2 (human) ( 2 )
Treatments:
AG490 ( 2 ) , anandamide ( 1 ) , mutation ( 2 ) , PRL ( 2 )

Downstream Regulation
Effects of modification on PRLR:
molecular association, regulation ( 3 , 4 ) , phosphorylation ( 4 ) , protein degradation ( 1 , 2 , 3 , 4 ) , receptor internalization, induced ( 2 ) , ubiquitination ( 2 )
Effects of modification on biological processes:
cell growth, altered ( 1 , 4 ) , cell motility, altered ( 1 ) , transcription, altered ( 1 , 4 )
Induce interaction with:
BTRC (human) ( 3 ) , FBW1B (human) ( 4 )

Disease / Diagnostics Relevance
Relevant diseases:
breast ductal carcinoma ( 3 )

References 

1

Plotnikov A, et al. (2009) Impaired turnover of prolactin receptor contributes to transformation of human breast cells. Cancer Res 69, 3165-72
19276348   Curated Info

2

Swaminathan G, et al. (2008) Prolactin stimulates ubiquitination, initial internalization, and degradation of its receptor via catalytic activation of Janus kinase 2. J Endocrinol 196, R1-7
18252943   Curated Info

3

Li Y, et al. (2006) Stabilization of prolactin receptor in breast cancer cells. Oncogene 25, 1896-902
16278670   Curated Info

4

Li Y, et al. (2004) Negative regulation of prolactin receptor stability and signaling mediated by SCF(beta-TrCP) E3 ubiquitin ligase. Mol Cell Biol 24, 4038-48
15082796   Curated Info