Ser36
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Home > Phosphorylation Site Page: > Ser36  -  IkB-alpha (mouse)

Site Information
RHDsGLDsMKDEEYE   SwissProt Entrez-Gene
Blast this site against: NCBI  SwissProt  PDB 
Site Group ID: 448204

In vivo Characterization
Methods used to characterize site in vivo:
2D analysis ( 2 ) , mutation of modification site ( 2 , 3 , 19 , 21 ) , phospho-antibody ( 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 ) , western blotting ( 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 )
Disease tissue studied:
lymphoma ( 20 ) , B cell lymphoma ( 20 )
Relevant cell line - cell type - tissue:
B lymphocyte-spleen ( 5 , 18 ) , bone marrow ( 17 ) , BV2 (microglia) ( 2 ) , C2C12 (myoblast) ( 11 ) , colon ( 8 , 15 ) , HEK293T (epithelial) ( 21 ) , liver ( 10 ) , macrophage-bone marrow ( 13 ) , macrophage-peritoneum ( 4 ) , MEF (fibroblast) ( 3 , 5 , 6 , 7 , 12 , 19 ) , MEF (fibroblast) [NFkB-p65 (mouse), homozygous knockout] ( 9 ) , microglia ( 2 ) , myeloid ( 13 ) , natural killer cell-spleen ( 14 ) , osteoclast ( 13 ) , skin ( 16 ) , spleen ( 17 ) , WEHI-231 (B lymphocyte) ( 20 )

Upstream Regulation
Regulatory protein:
GSK3B (human) ( 7 ) , IKKG (mouse) ( 3 ) , IL-1RA (mouse) ( 3 ) , SH3BP2 (mouse) ( 13 )
Putative in vivo kinases:
IKKB (mouse) ( 3 )
Kinases, in vitro:
IKKA (mouse) ( 12 ) , IKKB (mouse) ( 3 ) , IKKE (mouse) ( 22 ) , MST1 (mouse) ( 2 )
Treatments:
[6]-gingerol ( 16 ) , anti-CD40 ( 17 ) , anti-IgM ( 18 ) , anti-LT(beta)R ( 12 ) , Aplidin ( 19 ) , bacterial infection ( 15 ) , bortezomib ( 15 ) , cycloheximide ( 9 ) , EDA-A1 ( 20 ) , GM-CSF ( 14 ) , Go_6976 ( 18 ) , IL-10 ( 17 ) , IL-1b ( 9 ) , ionomycin ( 14 ) , LPS ( 4 , 9 , 14 , 17 ) , LY294002 ( 11 ) , M-CSF ( 13 ) , partial_hepatectomy ( 10 ) , phorbol_ester ( 14 , 16 ) , PIF ( 11 ) , SB203580 ( 16 ) , SC-514 ( 3 ) , TNF ( 6 , 9 , 12 ) , virus infection ( 7 )

Downstream Regulation
Effects of modification on IkB-alpha:
protein degradation ( 3 , 21 )

References 

1

Huang X, et al. (2021) Caspase inhibition prolongs inflammation by promoting a signaling complex with activated RIPK1. J Cell Biol 220
33914027   Curated Info

2

Zhao S, et al. (2016) Hippo/MST1 signaling mediates microglial activation following acute cerebral ischemia-reperfusion injury. Brain Behav Immun 55, 236-48
26721416   Curated Info

3

Schröfelbauer B, et al. (2012) NEMO Ensures Signaling Specificity of the Pleiotropic IKKβ by Directing Its Kinase Activity toward IκBα. Mol Cell 47, 111-21
22633953   Curated Info

4

Liu X, et al. (2011) Intracellular MHC class II molecules promote TLR-triggered innate immune responses by maintaining activation of the kinase Btk. Nat Immunol 12, 416-24
21441935   Curated Info

5

Tokunaga F, et al. (2011) SHARPIN is a component of the NF-κB-activating linear ubiquitin chain assembly complex. Nature 471, 633-6
21455180   Curated Info

6

Ju J, et al. (2010) Phosphorylation of p50 NF-kappaB at a single serine residue by DNA-dependent protein kinase is critical for VCAM-1 expression upon TNF treatment. J Biol Chem 285, 41152-60
20966071   Curated Info

7

Lei CQ, et al. (2010) Glycogen synthase kinase 3β regulates IRF3 transcription factor-mediated antiviral response via activation of the kinase TBK1. Immunity 33, 878-89
21145761   Curated Info

8

Chandrakesan P, et al. (2010) Novel changes in NF-{kappa}B activity during progression and regression phases of hyperplasia: role of MEK, ERK, and p38. J Biol Chem 285, 33485-98
20710027   Curated Info

9

Geng H, et al. (2009) Phosphorylation of NF-kappaB p65 at Ser468 controls its COMMD1-dependent ubiquitination and target gene-specific proteasomal elimination. EMBO Rep 10, 381-6
19270718   Curated Info

10

Malato Y, et al. (2008) Hepatocyte-specific inhibitor-of-kappaB-kinase deletion triggers the innate immune response and promotes earlier cell proliferation during liver regeneration. Hepatology 47, 2036-50
18393321   Curated Info

11

Russell ST, Eley HL, Wyke SM, Tisdale MJ (2008) Involvement of phosphoinositide 3-kinase and Akt in the induction of muscle protein degradation by proteolysis-inducing factor. Biochem J 409, 751-9
17961125   Curated Info

12

Basak S, et al. (2007) A fourth IkappaB protein within the NF-kappaB signaling module. Cell 128, 369-81
17254973   Curated Info

13

Ueki Y, et al. (2007) Increased myeloid cell responses to M-CSF and RANKL cause bone loss and inflammation in SH3BP2 "cherubism" mice. Cell 128, 71-83
17218256   Curated Info

14

Bezbradica JS, et al. (2006) Granulocyte-macrophage colony-stimulating factor regulates effector differentiation of invariant natural killer T cells during thymic ontogeny. Immunity 25, 487-97
16949316   Curated Info

15

Wang Y, Xiang GS, Kourouma F, Umar S (2006) Citrobacter rodentium-induced NF-kappaB activation in hyperproliferating colonic epithelia: role of p65 (Ser536) phosphorylation. Br J Pharmacol 148, 814-24
16751795   Curated Info

16

Kim SO, et al. (2005) [6]-Gingerol inhibits COX-2 expression by blocking the activation of p38 MAP kinase and NF-kappaB in phorbol ester-stimulated mouse skin. Oncogene 24, 2558-67
15735738   Curated Info

17

Bhattacharyya S, et al. (2004) Immunoregulation of dendritic cells by IL-10 is mediated through suppression of the PI3K/Akt pathway and of IkappaB kinase activity. Blood 104, 1100-9
15113757   Curated Info

18

Blois JT, et al. (2004) B cell receptor-induced cAMP-response element-binding protein activation in B lymphocytes requires novel protein kinase Cdelta. J Biol Chem 279, 30123-32
15138267   Curated Info

19

Cuadrado A, et al. (2004) JNK activation is critical for Aplidin-induced apoptosis. Oncogene 23, 4673-80
15122339   Curated Info

20

Kayagaki N, et al. (2002) BAFF/BLyS receptor 3 binds the B cell survival factor BAFF ligand through a discrete surface loop and promotes processing of NF-kappaB2. Immunity 17, 515-24
12387744   Curated Info

21

Shirane M, et al. (1999) Common pathway for the ubiquitination of IkappaBalpha, IkappaBbeta, and IkappaBepsilon mediated by the F-box protein FWD1. J Biol Chem 274, 28169-74
10497169   Curated Info

22

Shimada T, et al. (1999) IKK-i, a novel lipopolysaccharide-inducible kinase that is related to IkappaB kinases. Int Immunol 11, 1357-62
10421793   Curated Info

23

Van Antwerp DJ, Verma IM (1996) Signal-induced degradation of I(kappa)B(alpha): association with NF-kappaB and the PEST sequence in I(kappa)B(alpha) are not required. Mol Cell Biol 16, 6037-45
8887633   Curated Info